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产品细节图:
| 产品编号 | bs-0086R |
| 英文名称 | TGF beta 1 |
| 中文名称 | 转化生长因子β1/TGF β1/TGF-β1抗体 |
| 别 名 | CED; DPD1; TGF beta 1; TGF beta; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor, beta 1. |
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Specific References (84) | bs-0086R has been referenced in 84 publications.
111 [IF=1.96] Kou, Wei, et al. ʺTransforming growth factor-β1 promotes Treg commitment in nasal polyposis after intranasal steroid treatment.ʺ Inflammation Research (2013): 1-7. ELISA ; Human. 222
111 [IF=4.52] Abdelmagid, Samir M., et al. "Mutation in Osteoactivin Reduces Bone Formation in Vivo and Osteoblast Differentiation in Vitro." The American Journal of Pathology (2014). WB ; Rat. 222
111 [IF=1.06] Erbas, O., et al. "Nephro-protective effect of granulocyte colony-stimulating factor in streptozotocin induced diabetic rats." Biotechnic & Histochemistry 0 (2014): 1-9. IHC-P ; Rat. 222
111 [IF=.94] Tang, Jin, et al. "Increased adenosine levels contribute to ischemic kidney fibrosis in the unilateral ureteral obstruction model." Experimental and Therapeutic Medicine. IHC-P ; Mouse. 222
111 [IF=3.23] Xue X, Chen Q, Zhao G, Zhao J-Y, Duan Z, Zheng P-S (2015) The Overexpression of TGF-β and CCN2 in Intrauterine Adhesions Involves the NF-κB Signaling Pathway. PLoS ONE 10(12): e0146159. IHC-P ; Human. 222
111 [IF=7.39] Ganguly, Rituparna, et al. "Anti-atherogenic Effect of Trivalent Chromium-loaded CPMV Nanoparticles in Human Aortic Smooth Muscle Cells under Hyperglycemic Conditions in vitro." Nanoscale (2016). WB ; Human. 222
111 [IF=1.88] Venter, Neil Grant, et al. "Use of platelet-rich plasma in deep second-and third-degree burns." Burns (2016). other ;
111 [IF=1.64] Eren, Kenan, et al. "The Suppression of Wound Healing Response with Sirolimus and Sunitinib Following Experimental Trabeculectomy in a Rabbit Model." Current Eye Research (2016): 1-10. IHC-P ; Rabbit. 222
111 [IF=1.89] Hu, Jianguo, Biao Zeng, and Xingwei Jiang. "The expression of marker for endometrial stem cell and fibrosis was increased in intrauterine adhesious."International journal of clinical and experimental pathology 8.2 (2015): 1525. IHC-P ; Mouse. 222
111 [IF=1.27] Sun, Zhenqiang, et al. "Immunological effect induced by mesenchymal stem cells in a rat liver transplantation model." Experimental and therapeutic medicine 10.2 (2015): 401-406. IHC-P ; Rat. 222
111 [IF=1.4] Zhu, Yi, et al. "High Molecular Weight Hyaluronic Acid Inhibits Fibrosis of Endometrium." Medical Science Monitor 22 (2016): 3438-3445. IHC-P ; Mouse. 222
111 [IF=5.29] Shang, Peijin, et al. "Acetyl-11-Keto-β-Boswellic Acid Attenuates Prooxidant and Profibrotic Mechanisms Involving Transforming Growth Factor-β1, and Improves Vascular Remodeling in Spontaneously Hypertensive Rats." Scientific Reports 6 (2016): 39809. IHC-P ; Rat. 222
111 [IF=1.56] Du, Jinghua, et al. "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." Molecular medicine reports 13.3 (2016): 2159-2166. WB ; Mouse. 222
111 [IF=2.55] Shen, Haitao, et al. "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." International Immunopharmacology 46 (2017): 16-22. IHC-P ; Mouse. 222
111 [IF=2.13] Mao, Rui, et al. "Efficacy of radiotherapy for the treatment of cystic echinococcosis in naturally infected sheep." Infectious Diseases of Poverty 6.1 (2017): 88. IHC-P ; Sheep. 222
111 [IF=2.45] Xiu, Zhi-Ming, et al. "1-Acetyl-5-phenyl-1H-pyrrol-3-ylacetate: an aldose reductase inhibitor for the treatment of diabetic nephropathy." Bioorganic & Medicinal Chemistry Letters (2017). WB ; Rat. 222
111 [IF=1.51] Erbas et al. Oxytocin alleviates cisplatin-induced renal damage in rats. (2014) Iran.J.Basic.Med.Sci. 17:747-52 IHC ; Rat. 222
111 [IF=1.922] Chi et al. Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. (2018) Mol.Med.Rep. 17:6337-6344 IHC ; Human. 222
111 [IF=1.92] Wang et al. Extracellular matrix promotes proliferation, migration and adhesion of airway smooth muscle cells in a rat model of chronic obstructive pulmonary disease via upregulation of the PI3K/AKT signaling pathway. (2018) Mol.Med.Rep. 18:3143-3152 WB ; Rat. 222
111 [IF=2.884] Li HT et al. CpG-ODNs and Budesonide Act Synergistically to Improve Allergic Responses in Combined Allergic Rhinitis and Asthma Syndrome Induced by Chronic Exposure to Ovalbumin by Modulating the TSLP-DC-OX40L Axis.Inflammation. 2018 Aug;41(4):1304-1320. IHC ; Mouse. 222
111 [IF=5.5] Zhang et al. Cardiac Contractility Modulation Attenuate Myocardial Fibrosis by Inhibiting TGF-β1/Smad3 Signaling Pathway in a Rabbit Model of Chronic Heart Failure. (2016) Cell.Physiol.Biochem. 39:294-306 WB ; Rabbit. 222
111 [IF=7.901] Lin S et al. Strategy for hypertrophic scar therapy: Improved delivery of triamcinolone acetonide using mechanically robust tip-concentrated dissolving microneedle array. J Control Release. 2019 May 27;306:69-82. WB ; Rabbit. 222
111 [IF=3.367] Liu M et al. The effects of CRISPR-Cas9 knockout of the TGF-β1 gene on antler cartilage cells in vitro. Cell Mol Biol Lett. 2019 Jun 22;24:44. WB ; Deer. 222
111 [IF=0] Afdal A et al. The Expression of Transforming Growth Factor Beta-1 and Interleukin-6 on Human Prostate: Prostate Hyperplasia and Prostate Cancer. Open Access Maced J Med Sci. 2019 Jun 30;7(12):1905-1910. IHC-P ; Human. 222
111 [IF=3.571] Zheng HX et al. Cyanidin-3-glucoside from Black Rice Ameliorates Diabetic Nephropathy via Reducing Blood Glucose, Suppressing Oxidative Stress and Inflammation, and Regulating Transforming Growth Factor β1/Smad Expression. J Agric Food Chem. 2020 Apr 15;6 IHC-P ; Rat. 222
111 [IF=3.701] S Q et al. Cyanidin-3-glucoside from black rice prevents renal dysfunction and renal fibrosis in streptozotocin-diabetic rats. Journal of Functional Foods,(2020)72, 104062. IHC-P ; Rat. 222
111 [IF=4.679] Le G et al. Ochratoxin A induces glomerular injury through activating the ERK/NF-κB signaling pathway. Food Chem Toxicol. 2020 Sep;143:111516. WB&ICF ; Human. 222
111 [IF=1.813] Li Y et al. Study on the Anticancer Effect of an Astragaloside- and Chlorogenic Acid-Containing Herbal Medicine (RLT-03) in Breast Cancer. Evid Based Complement Alternat Med. 2020 Jun 10;2020:1515081. IHC-P ; Human. 222
111 [IF=4.679] Le G et al. Ochratoxin A Induces Glomerular Injury Through Activating the ERK/NF-κB Signaling Pathway. Food Chem Toxicol. 2020 Jun 29;111516. WB,IF ; human. 222
111 [IF=4.679] Guannan Leet al. Ochratoxin A induces glomerular injury through activating the ERK/NF-κB signaling pathway. Food Chem Toxicol . 2020 Sep;143:111516. WB ; Human. 222
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| 研究领域 | 肿瘤 心血管 信号转导 干细胞 生长因子和激素 转录调节因子 细胞因子 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, ) |
| 产品应用 |
WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12.8/44kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TGF-Beta 1: 351-390/390 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016] Function: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Subunit: Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the'small latent TGF-beta1 complex'. Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3 (By similarity). May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition (By similarity). Interacts with CD109, DPT and ASPN. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. Post-translational modifications: Glycosylated. The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. DISEASE: Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. Similarity: Belongs to the TGF-beta family. SWISS: P01137 Gene ID: 7040 Database links: Entrez Gene: 7040 Human Entrez Gene: 21803 Mouse Omim: 190180 Human SwissProt: P01137 Human SwissProt: P04202 Mouse Unigene: 645227 Human 生长因子和激素（ Growth Factor and Hormones） TGF是一种多效生长因子，对上皮细胞增值有潜在抑制作用，可抑制肿瘤生长用于许多恶性肿瘤如:胃癌、肺癌、膀胱癌、肾癌、前列腺癌、结肠癌等多种恶性肿瘤的研究。 TGFβ超级家族由为数众多的生长的分化因子组成，包括转移生长因子β1，2和3(TGF β1，TGF β2，TGF β3)；胎盘生长因子(PTGF-β)；生长/分化因子(GDFs)；缪氏抑制物(MIS)；骨形态形成蛋白(BMPs)；交织细胞元神经生长因子(GDNF)；抑制素和活化素(α, β-A,和β-C)，Lefty和Nodal。 TGF超级家族成员参与胚胎发育和成体组织体内平衡。TGF-β1抗体与TGF-β2和TGF-β3没有交叉反应。与豚鼠有大部分交叉。 |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug Liver (Rat) Lysate at 40 ug Primary: Anti- TGF beta 1 (bs-0086R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/44 kD Observed band size: 55 kD 
Sample:
Ovary (Mouse) Lysate at 40 ug Primary: Anti-TGF beta 1 (bs-0086R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/44 kD Observed band size: 59 kD 
Sample:
Embryo (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti-TGF beta 1 (bs-0086R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/44 kD Observed band size: 59 kD 
Sample:TGF beta1 recombinant protein (human) Lysate at 100ng
Primary: Anti- TGF beta1 (bs-0086R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/44 kD Observed band size: 12.8 kD 
Sample:
Lane 1: Stomach (Mouse) Lysate at 40 ug Lane 2: Liver (Mouse) Lysate at 40 ug Lane 3: Placenta (Mouse) Lysate at 40 ug Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 5: Liver (Rat) Lysate at 40 ug Primary: Anti-TGF beta 1 (bs-0086R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50/25/12.5 kD Observed band size: 50 kD 
Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TGF beta 1) Polyclonal Antibody, Unconjugated (bs-0086R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Blank control:MG63.
Primary Antibody (green line): Rabbit Anti-TGF beta 1 antibody (bs-0086R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
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