产品简介:SDS-PAGE 上样缓冲液(SDS-PAGE Loading Buffer, 5X),是一种经过改良的以溴酚蓝为染料,5 倍浓缩的蛋白上样缓冲液,用于 SDS-PAGE 时蛋白样品的上样操作。不含传统的 β-巯基乙醇或 DTT,Genefist 上样缓冲液采用 TCEP 作为还原剂,稳定性更好、还原效果更优,并且无难闻气味!保存条件:-20℃保存,至少一年有效。使用说明:1、在室温或不超过 37℃的水浴中溶解蛋白上样缓冲液。水浴溶解后立即室温存放,尽量避免长时间置于水浴中。使用完毕后置于-20℃保存。2、按照每 4ul 蛋白样品加入 1ul 上样缓冲液的比例,混合蛋白样品和上样缓冲液。3、100℃或沸水浴加热 5-10 分钟,以充分变性蛋白。4、冷却到室温后,上样到 SDS-PAGE 胶加样孔内,开始电泳。注意事项: 由于含有变性剂,本品不适用于非变性胶的电泳 SDS-PAGE 蛋白上样缓冲液(5X)必须完全溶解后再使用。 如果起始时细胞或组织的用量较大,基因组 DNA 含量较高,“混合液”可适当延长加热时间。 本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗。
产品简介在常规的 Western blot 和 ELISA 实验中,需要用封闭试剂将膜或酶标板孔中的未被抗原或抗体占据的位点进行封闭, 通常的封闭试剂有 BSA,脱脂奶粉等,但是 BSA 中有少量的抗体残留,导致抗原/抗体之间的交叉反应,从而产生高背景,杂带多,或本底水平增高等影响。同样脱脂奶粉中含有少量的生物素和碱性磷酸酶残留,在使用生物素-亲和素系统和碱性磷酸酶标记的二抗时,也会使得背景高或本底水平增高,脱脂奶粉中含有的酪蛋白和碱性磷酸酶残留也使得其不适用于磷酸化蛋白的检测。本产品采用化学合成的高分子聚合物作为核心封闭试剂,能够避免 BSA,脱脂奶粉作为封闭试剂的缺陷,提高实验结果的精确性,增加条带清晰度;产品不含磷酸盐、叠氮钠或硫柳汞类防腐剂,但采用安全性更高的医疗防腐处理,可以长时间保存,减少了 western 或 ELISA实验中蛋白封闭液不耐保存现用现配的麻烦;本产品可提高免疫印迹反应的灵敏度,封闭时间仅需十分钟,大大缩短了实验过程,节省了宝贵实验时间。产品特色: 即用型封闭液,无需现用现配,4℃保质两年 封闭时间短,仅需 10 分钟 减少高背景和非特异性免疫反应,增强条带清晰度 适用所有抗体的封闭(ELISA、WB) 不含 BSA、脱脂奶粉等动物源蛋白 不含磷酸盐、叠氮钠或硫柳汞类防腐剂运输和保存条件:常温存储,有效期两年。操作步骤 : 取适量的无蛋白封闭液加入到合适的含有膜的容器中,将膜完全浸没,在室温或 37℃条件下,震荡约 10 分钟。 对于 ELISA 实验中,将无蛋白封闭液加入酶标板的每个孔中在室温或 37℃条件下,震荡约 10 分钟。 按照 Western blot 和 ELISA 实验的要求,继续进行下一步实验操作。注意事项1、使用后请旋紧瓶盖并放回 4℃存储。2、在进行封闭后可以使用 Genefist 的通用型抗体稀释液(GF1600)进行一抗和二抗的稀释,能进一步提高条带的清晰度,减少显色后的高背景和杂带的产生 。3、本品不含叠氮钠或硫柳汞类防腐剂,但还需小心操作避免皮肤直接接触。4、本试剂只能用于科研实验,请勿用于临床诊断及其它用途。
产品简介通用型抗体稀释液是一种即用型的,适合于稀释所有免疫抗体测定(Immunofluorescence, IHC, ELISA, WB)实验中的抗体。该试剂可用于一抗或二抗的稀释和配制,在 4℃保存和使用不少于六个月。稀释液中加入了多种抗体结合反应增强剂及稳定剂,能够增加抗体的溶解度和稳定性,增强免疫反应,降低非特异的背景颜色,提高实验的特异性和灵敏度,可反复使用,节约宝贵的抗体。试剂中不含有动物源的蛋白,适合于所有类型抗体稀释,包括 HRP,AP 标记的抗体。不含磷酸盐、叠氮钠或硫柳汞类防腐剂,安全性更高,。产品特色 即用型稀释液,无需配制 稀释后的抗体可以使用长达 6 个月 适用所有实验的抗体稀释(IF、IHC、ELISA、WB) 含抗体结合反应增强剂、稳定剂,效果更佳 不含 BSA、脱脂奶粉等动物源蛋白 不含磷酸盐、叠氮钠或硫柳汞类防腐剂运输和保存条件常温运输,4℃存储,有效期两年。操作步骤 : 根据抗体使用说明书,直接用本品对一抗或二抗进行适当倍数的稀释。 保存在抗体稀释液中的抗体可回收于 4℃保存,可反复使用多次,长达 6 个月以上,直至不能达到理想结果后丢弃。注意事项1、使用后请旋紧瓶盖,防止溶液挥发或与空气的物质发生化学反应,并放回 4℃保存。2、在进行抗体免疫反应前可以使用 Genefist 的高效 Western 封闭液(货号GF1815)进行封闭,能进一步提高条带的清晰度,减少显色后的高背景和杂带的产生 。3、本品不含叠氮钠或硫柳汞类防腐剂,但还需小心操作避免皮肤直接接触。4、本试剂只能用于科研实验,请勿用于临床诊断和其它用途。
产品简介通用型抗体稀释液是一种即用型的,适合于稀释所有免疫抗体测定(Immunofluorescence, IHC, ELISA, WB)实验中的抗体。该试剂可用于一抗或二抗的稀释和配制,在 4℃保存和使用不少于六个月。稀释液中加入了多种抗体结合反应增强剂及稳定剂,能够增加抗体的溶解度和稳定性,增强免疫反应,降低非特异的背景颜色,提高实验的特异性和灵敏度,可反复使用,节约宝贵的抗体。试剂中不含有动物源的蛋白,适合于所有类型抗体稀释,包括 HRP,AP 标记的抗体。不含磷酸盐、叠氮钠或硫柳汞类防腐剂,安全性更高,。产品特色 即用型稀释液,无需配制 稀释后的抗体可以使用长达 6 个月 适用所有实验的抗体稀释(IF、IHC、ELISA、WB) 含抗体结合反应增强剂、稳定剂,效果更佳 不含 BSA、脱脂奶粉等动物源蛋白 不含磷酸盐、叠氮钠或硫柳汞类防腐剂运输和保存条件常温运输,4℃存储,有效期两年。操作步骤 : 根据抗体使用说明书,直接用本品对一抗或二抗进行适当倍数的稀释。 保存在抗体稀释液中的抗体可回收于 4℃保存,可反复使用多次,长达 6 个月以上,直至不能达到理想结果后丢弃。注意事项1、使用后请旋紧瓶盖,防止溶液挥发或与空气的物质发生化学反应,并放回 4℃保存。2、在进行抗体免疫反应前可以使用 Genefist 的高效 Western 封闭液(货号GF1815)进行封闭,能进一步提高条带的清晰度,减少显色后的高背景和杂带的产生 。3、本品不含叠氮钠或硫柳汞类防腐剂,但还需小心操作避免皮肤直接接触。4、本试剂只能用于科研实验,请勿用于临床诊断和其它用途。
半干转膜系统快速转膜液(5X)Cat #. GF1816 / Size. 500 ml产品简介半干转膜系统快速转膜液 Western blot Rapid Transfer Buffer for semi-dry(5X)是 Genefist推出的一种 5 倍浓度的高效转膜液,能高效快速地将蛋白转移到印迹膜(PVDF 或硝酸纤维素膜)上。本转膜液使用过程中,无需再使用甲醇或乙醇等任何醇类试剂,且能在 5-15 分钟内完成转膜过程,快速、高效、安全!产品特色: 高效安全:不使用甲醇或乙醇等任何醇类试剂,减少有毒试剂使用; ˗ 快速稳定:能在 5-15 分钟完成转膜过程,产热量小,减少蛋白损失; 兼容性好:兼容 Laemmli 胶,预制胶,FastPAGE,Bis-Tris 胶等多种凝胶。运输和保存条件:常温运输,室温存储,有效期一年。操作步骤 :1、1X 快速转膜液的配制(100ml):取 20ml 快速转膜液(5×)+ 80ml 去离子水;2、做好转印三明治结构,然后转移至电泳槽中,设定恒压 20V,一般时间 5-15 分钟完成蛋白转膜。备注: PVDF 膜使用前要用无水甲醇润湿 30 秒以上。 转印电流建议设定恒压 20V,1mm 厚度的凝胶,一般 5 分钟可以将 150kD 以下蛋白完全转印;大于 150kD 蛋白 10-15 分钟即可完全转印。 凝胶的浓度影响转印的效率,对于胶浓度 7.5%,5-10 分钟即可完成转印;对于胶浓度 10%,,5-15 分钟即可完成转印;对于胶浓度大于 10%,建议延长至 8-20 分钟。注意事项1、使用本试剂前请仔细阅读说明书。2、本产品只能用于科研实验,请勿用于临床诊断及其它用途。
产品简介Genefist 快速转膜液(10X)是 Genefist 研发的一款 10 倍浓缩的高效转膜液,转膜过程无需使用甲醇,且仅需 20-30 分钟,就能快速、高效地将蛋白转移到印迹膜(PVDF 或硝纤膜)上。Genefist 快速转膜液(10X),快速、安全、高效!产品特色: 安全便捷:不使用甲醇,减少有毒试剂使用; 快速高效:能在 20-30 分钟完成转膜过程,产热量小,减少蛋白损失; 兼容性好:兼容 Laemmli 胶,预制胶,FastPAGE,Bis-Tris 胶等多种凝胶。运输和保存条件:常温运输,室温存储,有效期两年。操作步骤 :1、1X 快速转膜液的配制(1000 ml):取 100ml 快速转膜液(10x)+ 700ml 去离子水 + 200ml无水乙醇。2、做好转印三明治结构,然后转移至电泳槽中,设定恒流 400mA,转印 20-30 分钟完成蛋白转膜。注意:(1)PVDF 膜使用前要用甲醇润湿 30 秒以上。(2)转印电流建议设定恒流 400mA,一般 20 分钟即可将 150kD 以下蛋白完全转印,对于更大分子量蛋白,可适当延长 5-15 分钟转膜时间。注意事项1、为了您的安全和健康,请穿实验服并戴一次性手套操作。2、本产品只能用于科研实验,请勿用于临床诊断及其它用途。
Product Information GeneFist ™series3-color High Range Protein Marker(9-245 kDa)GF6619Size:250 µl × 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6619 GeneFist™ 3-color High Range ProteinMarker is a ready to use three-color protein standardwith 12 pre-stained proteins covering a wide range ofmolecular weights from 10 to 245 kDa in Tris-Glycinebuffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins arecovalently coupled with different chromophores foreasy identification of bands, with two referenceproteins carrying enhanced intensity correspondingto a green at 25 kDa and red at 75 kDa, respectively,as separated on SDS-PAGE (Tris-Glycine buffer).The GF6619 GeneFist™ 3-color High Range ProteinMarker is designed for monitoring protein separationduring SDS-polyacrylamide gel electrophoresis,verification of Western transfer efficiency onmembranes (PVDF, nylon, or nitrocellulose) and forapproximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6619 GeneFist™ 3-color High Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue2452352352Blue1801701703Blue1401301304Blue10093935Red7570726Blue6053537Blue4541428Blue3530309Green25222310Blue20181811Blue15141412Blue109103-color High Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6619 GeneFist™ 3-color High Range Protein Marker resolves 12 majorbands in SDS-PAGE (Tris-Glycine, MOPS, and MESbuffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist 3-color Regular Range ProteinMarker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only
Product Information GeneFist ™series3-color High Range Protein Marker(9-245 kDa)GF6619Size:250 µl × 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6619 GeneFist™ 3-color High Range ProteinMarker is a ready to use three-color protein standardwith 12 pre-stained proteins covering a wide range ofmolecular weights from 10 to 245 kDa in Tris-Glycinebuffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins arecovalently coupled with different chromophores foreasy identification of bands, with two referenceproteins carrying enhanced intensity correspondingto a green at 25 kDa and red at 75 kDa, respectively,as separated on SDS-PAGE (Tris-Glycine buffer).The GF6619 GeneFist™ 3-color High Range ProteinMarker is designed for monitoring protein separationduring SDS-polyacrylamide gel electrophoresis,verification of Western transfer efficiency onmembranes (PVDF, nylon, or nitrocellulose) and forapproximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6619 GeneFist™ 3-color High Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue2452352352Blue1801701703Blue1401301304Blue10093935Red7570726Blue6053537Blue4541428Blue3530309Green25222310Blue20181811Blue15141412Blue109103-color High Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6619 GeneFist™ 3-color High Range Protein Marker resolves 12 majorbands in SDS-PAGE (Tris-Glycine, MOPS, and MESbuffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist 3-color Regular Range ProteinMarker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only