Product Information GeneFist ™ series3-color Regular Range Protein Marker(9-180 kDa)GF6616Size:250 µl x 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6616 GeneFist™ 3-color Regular RangeProtein Marker is a ready to use three-color proteinstandard with 10 pre-stained proteins covering a widerange of molecular weights from 10 to 180 kDa (9 to170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDain Bis-Tris (MES) buffer). Proteins are covalentlycoupled with a blue chromophore except for tworeference bands (one green and one red band at 25kDa and 75 kDa respectively) when separated on SDSPAGE (Tris-Glycine buffer).The GF6616 GeneFist™ 3-color Regular RangeProtein Marker is designed for monitoring proteinseparation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiencyon membranes, (PVDF, nylon, or nitrocellulose) andfor approximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6616 GeneFist™ 3-color Regular Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue1801701702Blue1401301303Blue10093934Red7570725Blue6053536Blue4541427Blue3530308Green2522239Blue15141410Blue109103-color Regular Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6616 GeneFist™3-color Regular Range Protein Marker resolves 10major bands in SDS-PAGE (Tris-Glycine, MOPS, andMES buffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist Enhanced 3-color Regular RangeProtein Marker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only
产品信息 PAGE预混胶试剂盒 CAT.# GF1800 分离胶A (200ml) 分离胶B (200ml) 浓缩胶A (75ml) 浓缩胶B (75ml) 产品描述 Ø 此试剂盒用于制备PAGE凝胶,快速、便捷;Ø 所制备的凝胶可用于变性和非变形蛋白质电泳分析;Ø 有6%、8%、10%、12%、15%五个不同浓度可供选择;Ø 预混配方,无需计算所需溶液量,无需稀释,只需两种液体等比例混合,简化传统制胶流程,缩短制胶时间;Ø 可耐受最高260V分离电压,将传统的凝胶电泳实验从1-2小时缩短至30分钟,且条带清晰、锐利;Ø 高效的蛋白转移能力,保证Western blot 数据的高度可靠性;Ø 每盒最多可制胶100块,超高性价比;Ø 不用冷藏或冷冻存贮,室温下保质期1年。使用说明: 1、 将分离胶I和分离胶II等比例混合(参照附表1),然后将所需的TEMED和10% APS加到混合溶液中(参照附表1),并混合均匀,灌入制胶板内。2、 在制胶板加入适量的醇或水压平分离胶,待分离胶凝固后,倒去上层的水或醇。3、 将浓缩胶I和浓缩胶II等比例混合(参照附表1),然后将所需的TEMED和10% APS加到混合溶液中(参照附表1),并混合均匀。4、 将配置好的浓缩胶预混液加入制作好分离胶的胶板内,缓慢插入干净的梳齿。 5、 待浓缩胶凝固后,拔去梳齿即可用于电泳。备注: 1、 此试剂盒常温存放即可;2、 TEMED和10%APS已包含在试剂盒中,请尽量使用新鲜配制的APS溶液; 3、 在制备分离胶和浓缩胶溶液时,注意避免气泡的产生;4、 制备好的凝胶可以用一张用去离子水浸湿的纸巾盖住,并将其储存在一个密封的塑料袋中,4 ℃可保存四个星期;5、 每个试剂盒可制备的凝胶数量:玻璃板大(9*7cm) 0.75 mm 玻璃板 1.0 mm 玻璃板 1.5 mm 玻璃板 制胶数 100块胶 65块胶 45块胶 关于凝胶速度说明: Ø 实验证明,凝胶速度与温度有显著的正相关性。同等条件下,温度越高,凝胶速度越快。比如25℃时凝胶显著快于15℃。同理,在37℃时,凝胶会进一步加快。如果要加速凝胶,可考虑提高凝胶温度(把胶放进37°培养箱)。Ø APS容易分解失效,APS的效力直接影响凝胶快慢,请尽量使用新配制的APS溶液;另外,如果制作的板子漏胶,也会影响分离胶的凝胶。Ø APS和TEMED在整个凝胶过程起催化加速作用,但添加过多,胶的柔性会降低。“附表1”APS和TEMED的添加量,正常情况会使凝胶在半小时内完成,并确保胶的柔韧性。关于电泳电压的说明: Ø 本试剂盒最适电压为160-200V,可承受的最高分离电压为260V,建议客户根据自己的实验条件和实验目选择电泳电压。附表1:制备胶所需体积列表 0.75mm 玻璃板 (n=制备胶个数) 1.0mm 玻璃板 (n=制备胶个数) 1.5mm 玻璃板 (n=制备胶个数) 浓缩胶 分离胶 浓缩胶 分离胶 浓缩胶 分离胶 分离胶I / 2 ml x n / 3 ml x n / 4 ml x n 分离胶II / 2 ml x n / 3 ml x n / 4 ml x n 浓缩胶I 1 ml x n / 1 ml x n / 1.5 ml x n / 浓缩胶II 1 ml x n / 1 ml x n / 1.5ml x n / TEMED 2 µl x n 2 µl x n 2 µl x n 3 µl x n 3 µl x n 4 µl x n 10% APS 20 µl x n 40 µl x n 20 µl x n 60 µl x n 30 µl x n 80 µl x n
产品描述 Ø 此试剂盒用于制备PAGE凝胶,快速、便捷;Ø 所制备的凝胶可用于变性和非变形蛋白质电泳分析;Ø 有6%、8%、10%、12%、15%五个不同浓度可供选择;Ø 预混配方,无需计算所需溶液量,无需稀释,只需两种液体等比例混合,简化传统制胶流程,缩短制胶时间;Ø 可耐受最高260V分离电压,将传统的凝胶电泳实验从1-2小时缩短至30分钟,且条带清晰、锐利;Ø 高效的蛋白转移能力,保证Western blot 数据的高度可靠性;Ø 每盒最多可制胶100块,超高性价比;Ø 不用冷藏或冷冻存贮,室温下保质期1年。使用说明: 1、 将分离胶I和分离胶II等比例混合(参照附表1),然后将所需的TEMED和10% APS加到混合溶液中(参照附表1),并混合均匀,灌入制胶板内。2、 在制胶板加入适量的醇或水压平分离胶,待分离胶凝固后,倒去上层的水或醇。3、 将浓缩胶I和浓缩胶II等比例混合(参照附表1),然后将所需的TEMED和10% APS加到混合溶液中(参照附表1),并混合均匀。4、 将配置好的浓缩胶预混液加入制作好分离胶的胶板内,缓慢插入干净的梳齿。 5、 待浓缩胶凝固后,拔去梳齿即可用于电泳。备注: 1、 此试剂盒常温存放即可;2、 TEMED和10%APS已包含在试剂盒中,请尽量使用新鲜配制的APS溶液; 3、 在制备分离胶和浓缩胶溶液时,注意避免气泡的产生;4、 制备好的凝胶可以用一张用去离子水浸湿的纸巾盖住,并将其储存在一个密封的塑料袋中,4 ℃可保存四个星期;5、 每个试剂盒可制备的凝胶数量:玻璃板大(9*7cm) 0.75 mm 玻璃板 1.0 mm 玻璃板 1.5 mm 玻璃板 制胶数 100块胶 65块胶 45块胶 关于凝胶速度说明: Ø 实验证明,凝胶速度与温度有显著的正相关性。同等条件下,温度越高,凝胶速度越快。比如25℃时凝胶显著快于15℃。同理,在37℃时,凝胶会进一步加快。如果要加速凝胶,可考虑提高凝胶温度(把胶放进37°培养箱)。Ø APS容易分解失效,APS的效力直接影响凝胶快慢,请尽量使用新配制的APS溶液;另外,如果制作的板子漏胶,也会影响分离胶的凝胶。Ø APS和TEMED在整个凝胶过程起催化加速作用,但添加过多,胶的柔性会降低。“附表1”APS和TEMED的添加量,正常情况会使凝胶在半小时内完成,并确保胶的柔韧性。关于电泳电压的说明: Ø 本试剂盒最适电压为160-200V,可承受的最高分离电压为260V,建议客户根据自己的实验条件和实验目选择电泳电压。附表1:制备胶所需体积列表 0.75mm 玻璃板 (n=制备胶个数) 1.0mm 玻璃板 (n=制备胶个数) 1.5mm 玻璃板 (n=制备胶个数) 浓缩胶 分离胶 浓缩胶 分离胶 浓缩胶 分离胶 分离胶I / 2 ml x n / 3 ml x n / 4 ml x n 分离胶II / 2 ml x n / 3 ml x n / 4 ml x n 浓缩胶I 1 ml x n / 1 ml x n / 1.5 ml x n / 浓缩胶II 1 ml x n / 1 ml x n / 1.5ml x n / TEMED 2 µl x n 2 µl x n 2 µl x n 3 µl x n 3 µl x n 4 µl x n 10% APS 20 µl x n 40 µl x n 20 µl x n 60 µl x n 30 µl x n 80 µl x n