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Product Information GeneFist ™ series
3-color Regular Range Protein Marker
(9-180 kDa)
GF6616
Size:
250 µl x 1
5 µl per well for 100 applications
Storage
4°C for 3 months
-20°C for 24 months
Loading Volume
• 3 μl or 5 μl per loading for clear visualization
during electrophoresis on a 15-well or 10-well
mini-gel.
• 1.5~2.5 μl per well for general Western blotting
transfer.
• Apply more for a thicker (> 1.5 mm) or larger gel.Description
The GF6616 GeneFist™ 3-color Regular Range
Protein Marker is a ready to use three-color protein
standard with 10 pre-stained proteins covering a wide
range of molecular weights from 10 to 180 kDa (9 to
170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa
in Bis-Tris (MES) buffer). Proteins are covalently
coupled with a blue chromophore except for two
reference bands (one green and one red band at 25
kDa and 75 kDa respectively) when separated on SDS
PAGE (Tris-Glycine buffer).
The GF6616 GeneFist™ 3-color Regular Range
Protein Marker is designed for monitoring protein
separation during SDS-polyacrylamide gel electro
phoresis, verification of Western transfer efficiency
on membranes, (PVDF, nylon, or nitrocellulose) and
for approximating the size of proteins.
The marker is supplied in gel loading buffer and is
ready to use. Do NOT heat, dilute, or add reducing
agents before loading.Contents
Approximately 0.1~0.4 mg/ml of each protein in the
buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %
SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)
Glycerol).
Guide for Molecular Weight Estimation
Migration patterns and approximate MWs (kDa) of
GF6616 GeneFist™ 3-color Regular Range Protein
Marker in different electrophoresis conditions are
listed below:
Band
Color
TRIS
GLYCINE
BIS-TRIS
(MOPS)
BIS-TRIS
(MES)
1
Blue
180
170
170
2
Blue
140
130
130
3
Blue
100
93
93
4
Red
75
70
72
5
Blue
60
53
53
6
Blue
45
41
42
7
Blue
35
30
30
8
Green
25
22
23
9
Blue
15
14
14
10
Blue
10
9
103-color Regular Range Protein Marker
Note. The apparent molecular weight (kDa) of each protein has been
determined by calibration against an unstained protein standard;
supplemental data should be considered for more accurate
adjustments in different electrophoresis conditions.Quality Control
Under suggested conditions, GF6616 GeneFist™
3-color Regular Range Protein Marker resolves 10
major bands in SDS-PAGE (Tris-Glycine, MOPS, and
MES buffer) and after Western blotting transfer to a
nitrocellulose membrane.
Other information
GENEFIST Technology, Inc. claims all warranties with
respect to this document, expressed or implied,
including but not limited to those of merchantability
or fitness for a particular purpose. In no event shall
GENEFIST Technology, Inc. be liable, whether in
contract, tort, warranty, or under any statute or any
other basis for special, incidental, indirect, punitive,
multiple or consequential damages in connection with
or arising from this document, including but not
limited to the use thereof.
Caution: Not intended for human or animal diagnostic or therapeutic
uses.Related Products
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GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2
GF6616 GeneFist Enhanced 3-color Regular Range
Protein Marker, 250 µl × 2
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Marker, 250 µl × 2
GF6261 GeneFist Enhanced 3-color High Range
Protein Marker, 250 µl × 2
GF6270 GeneFist 3-color Broad Range Protein
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Marker, 250 µl × 2
GF6500 GeneFist 3-color Pre-stained Protein Ladder,
Regular Range, 250 µl × 2
GF6510 GeneFist 3-color Pre-stained Protein
Ladder, High Range, 250 µl × 2
GF6520 GeneFist 3-color Pre-stained Protein
Ladder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye
(Red, 1,000X), 1 ml
GF5110 GeneFist 50 bp DNA Ladder, 500 µl
GF5220 GeneFist 100 bp DNA Ladder II, 500 µl
GF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,
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GF3010 GeneFist Blue Light LED epi-illuminator,
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For research use only
1.《Multifunction Sr, Co and F co-doped microporous coating on titanium of antibacterial, angiogenic and osteogenic activities》
作者：Jianhong Zhou ,Lingzhou Zhao 影响因子：4.259
期刊：《Scientific Reports 6, Article number: 29069 (2016)》 PMID：27353337
作者：Jianhong Zhou ,Lingzhou Zhao 影响因子：4.259
期刊：《Scientific Reports 6, Article number: 29069 (2016)》 PMID：27353337
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