中文 
产品细节图:
| 产品编号 | bs-0578R |
| 英文名称 | Collagen I |
| 中文名称 | I型胶原蛋白抗体 |
| 别 名 | Collagen type I; Alpha 1 type I collagen; Alpha 2 type I collagen; COL1A1; COL1A2; Collagen I alpha 1 polypeptide; Collagen I alpha 2 polypeptide; Collagen Of Skin Tendon And Bone; Collagen Type 1; Collagen type I alpha 1; Collagen type I alpha 2; OI4; Osteogenesis Imperfecta Type IV; Pro alpha 1(I) collagen; Type I procollagen; CO1A1_HUMAN; Collagen alpha-1(II) chain; Alpha-1 type II collagen; Collagen alpha-1(II) chain; Chondrocalcin; collagen alpha-1(I) chain preproprotein; Collagen Ⅰ. 胶原蛋白1; 1型胶原蛋白; I型胶原; |
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Specific References (118) | bs-0578R has been referenced in 118 publications.
111 [IF=7.7] Yang, Fan, et al. "Strontium enhances osteogenic differentiation of mesenchymal stem cells and in vivo bone formation by activating Wnt/catenin signaling." Stem Cells 29.6 (2011): 981-991. Human, Rat. 222
111 [IF=3.82] Luo, Yang, et al. "Alendronate Retards the Progression of Lumbar Intervertebral Disc Degeneration in Ovariectomized Rats." Bone (2013). IHC-P ; Rat. 222
111 [IF=3.61] Xu, Zhen E., et al. "Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice." American Journal of Physiology-Renal Physiology 301.4 (2011): F713-F722. IHC-P ; Mouse. 222
111 [IF=3.55] Guo, Hong-Feng, et al. "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." Colloids and Surfaces B: Biointerfaces (2012). WB ; Mouse. 222
111 [IF=1.97] Zhang, Qiang-Ye, et al. "Administration of a novel penicillamine-bound membrane: a preventive and therapeutic treatment for abdominal adhesions." BMC surgery 11.1 (2011): 5. Rat. 222
111 [IF=2.16] Zhou, Nan, et al. "Involvement of Imperatorin Derivative‐OW1 in the antihypertensive effect and vascular remodeling of renovascular hypertension rats." Clinical and Experimental Pharmacology and Physiology (2014). IHC-P ; Rat. 222
111 [IF=4.45] Wang, Chen-Chie, et al. "Expandable Scaffold Improves Integration of Tissue Engineered Cartilage: An In Vivo Study in A Rabbit Model." Tissue Engineering ja (2016). IHC-P ; Rabbit. 222
111 [IF=2.07] Chen, Cheng, et al. "Effects of Cytoskeleton Modulation on the Phenotype of Articular Chondrocytes in Monolayer and Pellet Culture." Journal of Biomaterials and Tissue Engineering 5.12 (2015): 942-950. IHC-P ; Rabbit. 222
111 [IF=1.93] Gao, Yuanyuan, et al. "TGF‐β1 promotes bovine mammary fibroblast proliferation through the ERK 1/2 signalling pathway." Cell biology international (2016). IHC-P ; Bovine. 222
111 [IF=.68] Çakir-Özkan, Nilüfer, et al. "Therapeutic Effects of Sildenafil on Experimental Mandibular Fractures." Journal of Craniofacial Surgery 27.3 (2016): 615-620. IHC-P ; Rat. 222
111 [IF=3.42] Cecen, Berivan, et al. "Biocompatibility and biomechanical characteristics of loofah based scaffolds combined with hydroxyapatite, cellulose, poly-l-lactic acid with chondrocyte-like cells." Materials Science and Engineering: C(2016). IHC ; Human. 222
111 [IF=1.68] Ren, Xiang, et al. "Engineering zonal cartilage through bioprinting collagen type II hydrogel constructs with biomimetic chondrocyte density gradient."BMC Musculoskeletal Disorders 17.1 (2016): 1. IHC-P ; Rabbit. 222
111 [IF=1.28] Tian, Fa‑Ming, et al. "Orally administered simvastatin partially preserves lumbar vertebral bone mass but not integrity of intervertebral discs in ovariectomized rats." Experimental and Therapeutic Medicine. IHC-P ; Rat. 222
111 [IF=3.365] Yang, Ning, et al. "Caffeic acid phenethyl ester up-regulates antioxidant levels in hepatic stellate cell line T6 via an Nrf2-mediated mitogen activated protein kinases pathway." World J Gastroenterol 23.7 (2017): 1203-1214. WB ; Rat. 222
111 [IF=5.168] Yu, Chengyuan, et al. "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." Oncotarget 8.23 (2017): 37705. WB ; Dog. 222
111 [IF=2.134] Wang et al. The influence of hypoxia during different pregnancy stages on cardiac collagen accumulation in the adult offspring. (2014) Biomed.Res.In. 2014:419805 WB ; Rat. 222
111 [IF=.971] Yang et al. Isolation and biological characterization of tendon-derived stem cells from fetal bovine. (2016) In.Vitro.Cell.Dev.Biol.Ani. 52:846-56 IF(ICC) ; Rat. 222
111 [IF=1.922] Chi et al. Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. (2018) Mol.Med.Rep. 17:6337-6344 IHC ; Human. 222
111 [IF=3.952] Yang KC et al. Low-adhesive ethylene vinyl alcohol-based packaging to xenogeneic islet encapsulation for type 1 diabetes treatment.Biotechnol Bioeng. 2018 Sep;115(9):2341-2355. IHC-P ; Rat. 222
111 [IF=1.792] Huo YT et al. Methacholine induces extracellular matrix production by human airway smooth muscle cells through -catenin signaling.Respiratory Physiology & Neurobiology, 254, 55–63. IF ; Human. 222
111 [IF=3.234] Fan S et al. Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway.Life Sci. 2018 Aug 15;207:205-211. WB ; Rat. 222
111 [IF=2.25] Zhang X et al. Quercetin ameliorates pulmonary fibrosis by inhibiting SphK1/S1P signaling.Biochem Cell Biol. 2018 Dec;96(6):742-751. WB ; Mouse&Human. 222
111 [IF=2.634] Ma C et al. Identification and Multilineage Potential Research of a Novel Type of Adipose-Derived Mesenchymal Stem Cells from Goose Inguinal Groove. DNA Cell Biol. 2018 Sep;37(9):731-741. ICF ; Goose. 222
111 [IF=1.664] Gao S et al. Abnormal changes in the quantity and function of osteoblasts cultured in patients with myelodysplastic syndrome. Oncol Lett. 2018 Oct;16(4):4384-4390. ICC ; Human. 222
111 [IF=3.166] Wang Y et al. ASIC1a promotes high glucose and PDGF-induced hepatic stellate cell activation by inducing autophagy through CaMKKβ/ERK signaling pathway. (2018) Toxicol Lett. (18)31963-5 WB ; Rat. 222
111 [IF=4.427] Wu M et al. Synergistic effects of sulfur dioxide and polycyclic aromatic hydrocarbons on pulmonary pro-fibrosis via mir-30c-1-3p/transforming growth factor β type II receptor axis.(2018) Chemosphere 219 WB ; Mouse&Human. 222
111 [IF=3.373] Niu L et al. PLGA/PLCA casting and PLGA/PDPA electrospinning bilayer film for prevention of postoperative adhesion. (2018) J. Biomed. Mater. Res. Part B Appl. Biomater. IHC-P ; Rabbit. 222
111 [IF=1.564] Liu P et al. Matrine Suppresses Pancreatic Fibrosis by Regulating TGF-β/Smad Signaling in Rats. (2019) Yonsei Med. J. 60(1) IHC-P ; Rat. 222
111 [IF=3.909] Kara A et al. Bioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineering. Int J Biol Macromol. 2019 Feb 20;130:266-279. SEM, fluorescence microscopy&IHC ; Fish(Sparus aurata). 222
111 [IF=4.938] Li Y et al. Klotho recovery by genistein via promoter histone acetylation and DNA demethylation mitigates renal fibrosis in mice.J Mol Med (Berl). 2019 Apr;97(4):541-552. WB ; Mouse&Human. 222
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Rabbit, (predicted: Cow, Horse, Sheep, Monkey, ) |
| 产品应用 |
WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 130kDa |
| 细胞定位 | 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Collagen I C-terminal propeptide: 1321-1400/1464 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]. Function: Type I collagen is a member of group I collagen (fibrillar forming collagen). Subunit: Trimers of one alpha 2(I) and two alpha 1(I) chains. Interacts with MRC2. Interacts with TRAM2. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. Post-translational modifications: Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. DISEASE: Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age. Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations. Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta. Defects in COL1A1 are a cause of osteogenesis imperfecta type 2 (OI2) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency. Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta. Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta. Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture. Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF. Similarity: Belongs to the fibrillar collagen family. Contains 1 fibrillar collagen NC1 domain. Contains 1 VWFC domain. SWISS: P02452 Gene ID: 1277 Database links: · Entrez Gene: 1277 Human · Entrez Gene: 12842 Mouse · Entrez Gene: 100008952 Rabbit · Entrez Gene: 29393 Rat · Omim: 120150 Human · SwissProt: P02453 Cow · SwissProt: O46392 Dog · SwissProt: P02452 Human · SwissProt: P11087 Mouse · SwissProt: P02454 Rat · Unigene: 172928 Human · Unigene: 277735 Mouse · Unigene: 107239 Rat I型胶原是软骨基质中的一种结构蛋白 。 主要用于Ⅰ型胶原分布及变态反应方面的研究。 |
| 产品图片 |
Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-Collagen I (bs-0578R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130,110 kD Observed band size: 130 kD 
Sample:
Lane 1: Placenta (Mouse) Lysate at 40 ug Lane 2: U-2OS (Human) Cell Lysate at 30 ug Primary: Anti-Collagen I (bs-0578R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130,110 kD Observed band size: 130 kD 
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Collagen I Polyclonal Antibody, Unconjugated(bs-0578R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Collagen I Polyclonal Antibody, Unconjugated(bs-0578R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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1.《Multifunction Sr, Co and F co-doped microporous coating on titanium of antibacterial, angiogenic and osteogenic activities》
作者：Jianhong Zhou ,Lingzhou Zhao 影响因子：4.259
期刊：《Scientific Reports 6, Article number: 29069 (2016)》 PMID：27353337
作者：Jianhong Zhou ,Lingzhou Zhao 影响因子：4.259
期刊：《Scientific Reports 6, Article number: 29069 (2016)》 PMID：27353337
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