溶葡萄球菌酶 (≥90%, ≥3000 unit/mg) Lysostaphin from Staphylococcus simulans

溶葡萄球菌酶 (≥90%, ≥3000 unit/mg)  Lysostaphin from Staphylococcus simulans

别名：

CAS号：9011-93-2
分子量：27kDa
MDL：MFCD00131556
级别：BR
活性：≥3000 unit/mg solid
纯度：≥90% (HPLC)
外观/性状：lyophilized powder
酶活性定义：One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.
等电点：10.5-11.0。
酶活性最适pH范围：10～11。
影响因素：羟汞苯甲酸（PCMB）、 苯甲磺酰氯（PMSF）、二硫苏糖醇（DTT） 等在1mmol/L的条件下，对酶活力无明显影响，Zn离子表现出某种程度的活化效应；Ba离子、Mn离子、Ca离子、Mg离子和EDTA在1mmol/L的条件下，对溶葡球菌酶表现出一定程度的抑制作用
溶解性：可溶于50mmol/L Tris 缓冲液
来源：Recombinant, Expressed in E. coli.
用途：生化研究。专一降解金黄色葡萄球菌等细胞壁具有甘氨酸肽键结构的细菌，通过裂解其细胞壁达到杀菌的目的。在pH值为4和5℃时高度稳定。
产品描述：Lysostaphin from Staphylococcus simulans has been used in a study to assess molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus. It has also been used in a study to investigate the sequence analysis of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity.
A protein complex with highly specific lytic activity against Staphylococcus species, including Staphylococcus aureus.
Lysostaphin possesses a lytic action against Staphylococcus aureus, which is used to treat antibiotic-resistant staphylococcal infections. It has the properties of enzymes, such as glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acetyl muramyl-L-alanine amidase. Lysostaphin is also used as a preservative in food industry and in clinical labs for rapid screening. The antimicrobial activity of lysostaphin, a metalloendopeptidase expressed by Staphylococcus simulans, is enhanced by binding to the C′ terminus of the membrane-associated Trap protein of Staphylococcus aureus.