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Product Information GeneFist ™series
3-color High Range Protein Marker
(9-245 kDa)
GF6619
Size:
250 µl × 1
5 µl per well for 100 applications
Storage
4°C for 3 months
-20°C for 24 months
Loading Volume
• 3 μl or 5 μl per loading for clear visualization
during electrophoresis on a 15-well or 10-well
mini-gel.
• 1.5~2.5 μl per well for general Western blotting
transfer.
• Apply more for a thicker (> 1.5 mm) or larger gel.Description
The GF6619 GeneFist™ 3-color High Range Protein
Marker is a ready to use three-color protein standard
with 12 pre-stained proteins covering a wide range of
molecular weights from 10 to 245 kDa in Tris-Glycine
buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-
235 kDa in Bis-Tris (MES) buffer). Proteins are
covalently coupled with different chromophores for
easy identification of bands, with two reference
proteins carrying enhanced intensity corresponding
to a green at 25 kDa and red at 75 kDa, respectively,
as separated on SDS-PAGE (Tris-Glycine buffer).
The GF6619 GeneFist™ 3-color High Range Protein
Marker is designed for monitoring protein separation
during SDS-polyacrylamide gel electrophoresis,
verification of Western transfer efficiency on
membranes (PVDF, nylon, or nitrocellulose) and for
approximating the size of proteins.
The marker is supplied in gel loading buffer and is
ready to use. Do NOT heat, dilute, or add reducing
agents before loading.Contents
Approximately 0.1~0.4 mg/ml of each protein in the
buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %
SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)
Glycerol).
Guide for Molecular Weight Estimation
Migration patterns and approximate MWs (kDa) of
GF6619 GeneFist™ 3-color High Range Protein
Marker in different electrophoresis conditions are
listed below:
Band
Color
TRIS
GLYCINE
BIS-TRIS
(MOPS)
BIS-TRIS
(MES)
1
Blue
245
235
235
2
Blue
180
170
170
3
Blue
140
130
130
4
Blue
100
93
93
5
Red
75
70
72
6
Blue
60
53
53
7
Blue
45
41
42
8
Blue
35
30
30
9
Green
25
22
23
10
Blue
20
18
18
11
Blue
15
14
14
12
Blue
10
9
103-color High Range Protein Marker
Note. The apparent molecular weight (kDa) of each protein has been
determined by calibration against an unstained protein standard;
supplemental data should be considered for more accurate
adjustments in different electrophoresis conditions.Quality Control
Under suggested conditions, GF6619 GeneFist™ 3-
color High Range Protein Marker resolves 12 major
bands in SDS-PAGE (Tris-Glycine, MOPS, and MES
buffer) and after Western blotting transfer to a
nitrocellulose membrane.
Other information
GENEFIST Technology, Inc. claims all warranties with
respect to this document, expressed or implied,
including but not limited to those of merchantability
or fitness for a particular purpose. In no event shall
GENEFIST Technology, Inc. be liable, whether in
contract, tort, warranty, or under any statute or any
other basis for special, incidental, indirect, punitive,
multiple or consequential damages in connection with
or arising from this document, including but not
limited to the use thereof.
Caution: Not intended for human or animal diagnostic or therapeutic
uses.Related Products
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For research use only
1.《Multifunction Sr, Co and F co-doped microporous coating on titanium of antibacterial, angiogenic and osteogenic activities》
作者：Jianhong Zhou ,Lingzhou Zhao 影响因子：4.259
期刊：《Scientific Reports 6, Article number: 29069 (2016)》 PMID：27353337
作者：Jianhong Zhou ,Lingzhou Zhao 影响因子：4.259
期刊：《Scientific Reports 6, Article number: 29069 (2016)》 PMID：27353337
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