genefist，GF0100，琼脂糖

200.00元

genefist，GF0100，琼脂糖，100g

genefist，GF1811，5X SDS-PAGE蛋白上样缓冲液

250.00元

产品简介：SDS-PAGE 上样缓冲液(SDS-PAGE Loading Buffer, 5X)，是一种经过改良的以溴酚蓝为染料，5 倍浓缩的蛋白上样缓冲液，用于 SDS-PAGE 时蛋白样品的上样操作。不含传统的 β-巯基乙醇或 DTT，Genefist 上样缓冲液采用 TCEP 作为还原剂，稳定性更好、还原效果更优，并且无难闻气味！保存条件：-20℃保存，至少一年有效。使用说明：1、在室温或不超过 37℃的水浴中溶解蛋白上样缓冲液。水浴溶解后立即室温存放，尽量避免长时间置于水浴中。使用完毕后置于-20℃保存。2、按照每 4ul 蛋白样品加入 1ul 上样缓冲液的比例，混合蛋白样品和上样缓冲液。3、100℃或沸水浴加热 5-10 分钟，以充分变性蛋白。4、冷却到室温后，上样到 SDS-PAGE 胶加样孔内，开始电泳。注意事项： 由于含有变性剂，本品不适用于非变性胶的电泳 SDS-PAGE 蛋白上样缓冲液(5X)必须完全溶解后再使用。 如果起始时细胞或组织的用量较大，基因组 DNA 含量较高，“混合液”可适当延长加热时间。 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗。

genefist，GF1600-5，通用型抗体稀释液

789.00元

产品简介通用型抗体稀释液是一种即用型的，适合于稀释所有免疫抗体测定（Immunofluorescence, IHC, ELISA, WB）实验中的抗体。该试剂可用于一抗或二抗的稀释和配制，在 4℃保存和使用不少于六个月。稀释液中加入了多种抗体结合反应增强剂及稳定剂，能够增加抗体的溶解度和稳定性，增强免疫反应，降低非特异的背景颜色，提高实验的特异性和灵敏度，可反复使用，节约宝贵的抗体。试剂中不含有动物源的蛋白，适合于所有类型抗体稀释，包括 HRP，AP 标记的抗体。不含磷酸盐、叠氮钠或硫柳汞类防腐剂，安全性更高，。产品特色 即用型稀释液，无需配制 稀释后的抗体可以使用长达 6 个月 适用所有实验的抗体稀释（IF、IHC、ELISA、WB） 含抗体结合反应增强剂、稳定剂，效果更佳 不含 BSA、脱脂奶粉等动物源蛋白 不含磷酸盐、叠氮钠或硫柳汞类防腐剂运输和保存条件常温运输，4℃存储，有效期两年。操作步骤 ： 根据抗体使用说明书，直接用本品对一抗或二抗进行适当倍数的稀释。 保存在抗体稀释液中的抗体可回收于 4℃保存，可反复使用多次，长达 6 个月以上，直至不能达到理想结果后丢弃。注意事项1、使用后请旋紧瓶盖，防止溶液挥发或与空气的物质发生化学反应，并放回 4℃保存。2、在进行抗体免疫反应前可以使用 Genefist 的高效 Western 封闭液（货号GF1815）进行封闭，能进一步提高条带的清晰度，减少显色后的高背景和杂带的产生 。3、本品不含叠氮钠或硫柳汞类防腐剂，但还需小心操作避免皮肤直接接触。4、本试剂只能用于科研实验，请勿用于临床诊断和其它用途。

genefist，GF1600-1，通用型抗体稀释液

189.00元

产品简介通用型抗体稀释液是一种即用型的，适合于稀释所有免疫抗体测定（Immunofluorescence, IHC, ELISA, WB）实验中的抗体。该试剂可用于一抗或二抗的稀释和配制，在 4℃保存和使用不少于六个月。稀释液中加入了多种抗体结合反应增强剂及稳定剂，能够增加抗体的溶解度和稳定性，增强免疫反应，降低非特异的背景颜色，提高实验的特异性和灵敏度，可反复使用，节约宝贵的抗体。试剂中不含有动物源的蛋白，适合于所有类型抗体稀释，包括 HRP，AP 标记的抗体。不含磷酸盐、叠氮钠或硫柳汞类防腐剂，安全性更高，。产品特色 即用型稀释液，无需配制 稀释后的抗体可以使用长达 6 个月 适用所有实验的抗体稀释（IF、IHC、ELISA、WB） 含抗体结合反应增强剂、稳定剂，效果更佳 不含 BSA、脱脂奶粉等动物源蛋白 不含磷酸盐、叠氮钠或硫柳汞类防腐剂运输和保存条件常温运输，4℃存储，有效期两年。操作步骤 ： 根据抗体使用说明书，直接用本品对一抗或二抗进行适当倍数的稀释。 保存在抗体稀释液中的抗体可回收于 4℃保存，可反复使用多次，长达 6 个月以上，直至不能达到理想结果后丢弃。注意事项1、使用后请旋紧瓶盖，防止溶液挥发或与空气的物质发生化学反应，并放回 4℃保存。2、在进行抗体免疫反应前可以使用 Genefist 的高效 Western 封闭液（货号GF1815）进行封闭，能进一步提高条带的清晰度，减少显色后的高背景和杂带的产生 。3、本品不含叠氮钠或硫柳汞类防腐剂，但还需小心操作避免皮肤直接接触。4、本试剂只能用于科研实验，请勿用于临床诊断和其它用途。

genefist，GF1817，5X半干转快速转膜液

318.00元

半干转膜系统快速转膜液（5X）Cat #. GF1816 / Size. 500 ml产品简介半干转膜系统快速转膜液 Western blot Rapid Transfer Buffer for semi-dry（5X）是 Genefist推出的一种 5 倍浓度的高效转膜液，能高效快速地将蛋白转移到印迹膜（PVDF 或硝酸纤维素膜）上。本转膜液使用过程中，无需再使用甲醇或乙醇等任何醇类试剂，且能在 5-15 分钟内完成转膜过程，快速、高效、安全！产品特色： 高效安全：不使用甲醇或乙醇等任何醇类试剂，减少有毒试剂使用； ˗ 快速稳定：能在 5-15 分钟完成转膜过程，产热量小，减少蛋白损失； 兼容性好：兼容 Laemmli 胶，预制胶，FastPAGE，Bis-Tris 胶等多种凝胶。运输和保存条件：常温运输，室温存储，有效期一年。操作步骤 ：1、1X 快速转膜液的配制（100ml）：取 20ml 快速转膜液（5×）+ 80ml 去离子水；2、做好转印三明治结构，然后转移至电泳槽中，设定恒压 20V，一般时间 5-15 分钟完成蛋白转膜。备注： PVDF 膜使用前要用无水甲醇润湿 30 秒以上。 转印电流建议设定恒压 20V，1mm 厚度的凝胶，一般 5 分钟可以将 150kD 以下蛋白完全转印；大于 150kD 蛋白 10-15 分钟即可完全转印。 凝胶的浓度影响转印的效率，对于胶浓度 7.5%，5-10 分钟即可完成转印；对于胶浓度 10%,，5-15 分钟即可完成转印；对于胶浓度大于 10%，建议延长至 8-20 分钟。注意事项1、使用本试剂前请仔细阅读说明书。2、本产品只能用于科研实验，请勿用于临床诊断及其它用途。

genefist，GF6619-10，三色预染蛋白marker 10-245KD (protein marker)

2650.00元

Product Information GeneFist ™series3-color High Range Protein Marker(9-245 kDa)GF6619Size:250 µl × 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6619 GeneFist™ 3-color High Range ProteinMarker is a ready to use three-color protein standardwith 12 pre-stained proteins covering a wide range ofmolecular weights from 10 to 245 kDa in Tris-Glycinebuffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins arecovalently coupled with different chromophores foreasy identification of bands, with two referenceproteins carrying enhanced intensity correspondingto a green at 25 kDa and red at 75 kDa, respectively,as separated on SDS-PAGE (Tris-Glycine buffer).The GF6619 GeneFist™ 3-color High Range ProteinMarker is designed for monitoring protein separationduring SDS-polyacrylamide gel electrophoresis,verification of Western transfer efficiency onmembranes (PVDF, nylon, or nitrocellulose) and forapproximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6619 GeneFist™ 3-color High Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue2452352352Blue1801701703Blue1401301304Blue10093935Red7570726Blue6053537Blue4541428Blue3530309Green25222310Blue20181811Blue15141412Blue109103-color High Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6619 GeneFist™ 3-color High Range Protein Marker resolves 12 majorbands in SDS-PAGE (Tris-Glycine, MOPS, and MESbuffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist 3-color Regular Range ProteinMarker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only

genefist，GF6619-01，三色预染蛋白marker 10-245KD (protein marker)

300.00元

Product Information GeneFist ™series3-color High Range Protein Marker(9-245 kDa)GF6619Size:250 µl × 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6619 GeneFist™ 3-color High Range ProteinMarker is a ready to use three-color protein standardwith 12 pre-stained proteins covering a wide range ofmolecular weights from 10 to 245 kDa in Tris-Glycinebuffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins arecovalently coupled with different chromophores foreasy identification of bands, with two referenceproteins carrying enhanced intensity correspondingto a green at 25 kDa and red at 75 kDa, respectively,as separated on SDS-PAGE (Tris-Glycine buffer).The GF6619 GeneFist™ 3-color High Range ProteinMarker is designed for monitoring protein separationduring SDS-polyacrylamide gel electrophoresis,verification of Western transfer efficiency onmembranes (PVDF, nylon, or nitrocellulose) and forapproximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6619 GeneFist™ 3-color High Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue2452352352Blue1801701703Blue1401301304Blue10093935Red7570726Blue6053537Blue4541428Blue3530309Green25222310Blue20181811Blue15141412Blue109103-color High Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6619 GeneFist™ 3-color High Range Protein Marker resolves 12 majorbands in SDS-PAGE (Tris-Glycine, MOPS, and MESbuffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist 3-color Regular Range ProteinMarker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only

genefist，GF6616-10，三色预染蛋白marker 10-180KD (protein marker)

2350.00元

Product Information GeneFist ™ series3-color Regular Range Protein Marker(9-180 kDa)GF6616Size:250 µl x 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6616 GeneFist™ 3-color Regular RangeProtein Marker is a ready to use three-color proteinstandard with 10 pre-stained proteins covering a widerange of molecular weights from 10 to 180 kDa (9 to170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDain Bis-Tris (MES) buffer). Proteins are covalentlycoupled with a blue chromophore except for tworeference bands (one green and one red band at 25kDa and 75 kDa respectively) when separated on SDSPAGE (Tris-Glycine buffer).The GF6616 GeneFist™ 3-color Regular RangeProtein Marker is designed for monitoring proteinseparation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiencyon membranes, (PVDF, nylon, or nitrocellulose) andfor approximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6616 GeneFist™ 3-color Regular Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue1801701702Blue1401301303Blue10093934Red7570725Blue6053536Blue4541427Blue3530308Green2522239Blue15141410Blue109103-color Regular Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6616 GeneFist™3-color Regular Range Protein Marker resolves 10major bands in SDS-PAGE (Tris-Glycine, MOPS, andMES buffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist Enhanced 3-color Regular RangeProtein Marker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only

genefist，GF6616-01，三色预染蛋白marker 10-180KD (protein marker)

265.00元

Product Information GeneFist ™ series3-color Regular Range Protein Marker(9-180 kDa)GF6616Size:250 µl x 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6616 GeneFist™ 3-color Regular RangeProtein Marker is a ready to use three-color proteinstandard with 10 pre-stained proteins covering a widerange of molecular weights from 10 to 180 kDa (9 to170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDain Bis-Tris (MES) buffer). Proteins are covalentlycoupled with a blue chromophore except for tworeference bands (one green and one red band at 25kDa and 75 kDa respectively) when separated on SDSPAGE (Tris-Glycine buffer).The GF6616 GeneFist™ 3-color Regular RangeProtein Marker is designed for monitoring proteinseparation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiencyon membranes, (PVDF, nylon, or nitrocellulose) andfor approximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6616 GeneFist™ 3-color Regular Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue1801701702Blue1401301303Blue10093934Red7570725Blue6053536Blue4541427Blue3530308Green2522239Blue15141410Blue109103-color Regular Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6616 GeneFist™3-color Regular Range Protein Marker resolves 10major bands in SDS-PAGE (Tris-Glycine, MOPS, andMES buffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist Enhanced 3-color Regular RangeProtein Marker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only

genefist，GF1800-08，8% PAGE预混胶试剂盒

261.00元

产品信息 PAGE预混胶试剂盒 CAT.# GF1800 分离胶A (200ml) 分离胶B (200ml) 浓缩胶A (75ml) 浓缩胶B (75ml) 产品描述 Ø 此试剂盒用于制备PAGE凝胶，快速、便捷；Ø 所制备的凝胶可用于变性和非变形蛋白质电泳分析；Ø 有6%、8%、10%、12%、15%五个不同浓度可供选择；Ø 预混配方，无需计算所需溶液量，无需稀释，只需两种液体等比例混合，简化传统制胶流程，缩短制胶时间；Ø 可耐受最高260V分离电压，将传统的凝胶电泳实验从1-2小时缩短至30分钟，且条带清晰、锐利；Ø 高效的蛋白转移能力，保证Western blot 数据的高度可靠性；Ø 每盒最多可制胶100块，超高性价比；Ø 不用冷藏或冷冻存贮，室温下保质期1年。使用说明： 1、 将分离胶I和分离胶II等比例混合（参照附表1），然后将所需的TEMED和10% APS加到混合溶液中（参照附表1），并混合均匀，灌入制胶板内。2、 在制胶板加入适量的醇或水压平分离胶，待分离胶凝固后，倒去上层的水或醇。3、 将浓缩胶I和浓缩胶II等比例混合（参照附表1），然后将所需的TEMED和10% APS加到混合溶液中（参照附表1），并混合均匀。4、 将配置好的浓缩胶预混液加入制作好分离胶的胶板内，缓慢插入干净的梳齿。 5、 待浓缩胶凝固后，拔去梳齿即可用于电泳。备注： 1、 此试剂盒常温存放即可；2、 TEMED和10%APS已包含在试剂盒中，请尽量使用新鲜配制的APS溶液； 3、 在制备分离胶和浓缩胶溶液时，注意避免气泡的产生；4、 制备好的凝胶可以用一张用去离子水浸湿的纸巾盖住，并将其储存在一个密封的塑料袋中，4 ℃可保存四个星期；5、 每个试剂盒可制备的凝胶数量：玻璃板大（9*7cm） 0.75 mm 玻璃板 1.0 mm 玻璃板 1.5 mm 玻璃板 制胶数 100块胶 65块胶 45块胶 关于凝胶速度说明： Ø 实验证明，凝胶速度与温度有显著的正相关性。同等条件下，温度越高，凝胶速度越快。比如25℃时凝胶显著快于15℃。同理，在37℃时，凝胶会进一步加快。如果要加速凝胶，可考虑提高凝胶温度（把胶放进37°培养箱）。Ø APS容易分解失效，APS的效力直接影响凝胶快慢，请尽量使用新配制的APS溶液；另外，如果制作的板子漏胶，也会影响分离胶的凝胶。Ø APS和TEMED在整个凝胶过程起催化加速作用，但添加过多，胶的柔性会降低。“附表1”APS和TEMED的添加量，正常情况会使凝胶在半小时内完成，并确保胶的柔韧性。关于电泳电压的说明： Ø 本试剂盒最适电压为160-200V，可承受的最高分离电压为260V，建议客户根据自己的实验条件和实验目选择电泳电压。附表1：制备胶所需体积列表 0.75mm 玻璃板 (n=制备胶个数) 1.0mm 玻璃板 (n=制备胶个数) 1.5mm 玻璃板 (n=制备胶个数) 浓缩胶 分离胶 浓缩胶 分离胶 浓缩胶 分离胶 分离胶I / 2 ml x n / 3 ml x n / 4 ml x n 分离胶II / 2 ml x n / 3 ml x n / 4 ml x n 浓缩胶I 1 ml x n / 1 ml x n / 1.5 ml x n / 浓缩胶II 1 ml x n / 1 ml x n / 1.5ml x n / TEMED 2 µl x n 2 µl x n 2 µl x n 3 µl x n 3 µl x n 4 µl x n 10% APS 20 µl x n 40 µl x n 20 µl x n 60 µl x n 30 µl x n 80 µl x n

genefist，GF1800-06，6% PAGE预混胶试剂盒

279.00元

产品描述 Ø 此试剂盒用于制备PAGE凝胶，快速、便捷；Ø 所制备的凝胶可用于变性和非变形蛋白质电泳分析；Ø 有6%、8%、10%、12%、15%五个不同浓度可供选择；Ø 预混配方，无需计算所需溶液量，无需稀释，只需两种液体等比例混合，简化传统制胶流程，缩短制胶时间；Ø 可耐受最高260V分离电压，将传统的凝胶电泳实验从1-2小时缩短至30分钟，且条带清晰、锐利；Ø 高效的蛋白转移能力，保证Western blot 数据的高度可靠性；Ø 每盒最多可制胶100块，超高性价比；Ø 不用冷藏或冷冻存贮，室温下保质期1年。使用说明： 1、 将分离胶I和分离胶II等比例混合（参照附表1），然后将所需的TEMED和10% APS加到混合溶液中（参照附表1），并混合均匀，灌入制胶板内。2、 在制胶板加入适量的醇或水压平分离胶，待分离胶凝固后，倒去上层的水或醇。3、 将浓缩胶I和浓缩胶II等比例混合（参照附表1），然后将所需的TEMED和10% APS加到混合溶液中（参照附表1），并混合均匀。4、 将配置好的浓缩胶预混液加入制作好分离胶的胶板内，缓慢插入干净的梳齿。 5、 待浓缩胶凝固后，拔去梳齿即可用于电泳。备注： 1、 此试剂盒常温存放即可；2、 TEMED和10%APS已包含在试剂盒中，请尽量使用新鲜配制的APS溶液； 3、 在制备分离胶和浓缩胶溶液时，注意避免气泡的产生；4、 制备好的凝胶可以用一张用去离子水浸湿的纸巾盖住，并将其储存在一个密封的塑料袋中，4 ℃可保存四个星期；5、 每个试剂盒可制备的凝胶数量：玻璃板大（9*7cm） 0.75 mm 玻璃板 1.0 mm 玻璃板 1.5 mm 玻璃板 制胶数 100块胶 65块胶 45块胶 关于凝胶速度说明： Ø 实验证明，凝胶速度与温度有显著的正相关性。同等条件下，温度越高，凝胶速度越快。比如25℃时凝胶显著快于15℃。同理，在37℃时，凝胶会进一步加快。如果要加速凝胶，可考虑提高凝胶温度（把胶放进37°培养箱）。Ø APS容易分解失效，APS的效力直接影响凝胶快慢，请尽量使用新配制的APS溶液；另外，如果制作的板子漏胶，也会影响分离胶的凝胶。Ø APS和TEMED在整个凝胶过程起催化加速作用，但添加过多，胶的柔性会降低。“附表1”APS和TEMED的添加量，正常情况会使凝胶在半小时内完成，并确保胶的柔韧性。关于电泳电压的说明： Ø 本试剂盒最适电压为160-200V，可承受的最高分离电压为260V，建议客户根据自己的实验条件和实验目选择电泳电压。附表1：制备胶所需体积列表 0.75mm 玻璃板 (n=制备胶个数) 1.0mm 玻璃板 (n=制备胶个数) 1.5mm 玻璃板 (n=制备胶个数) 浓缩胶 分离胶 浓缩胶 分离胶 浓缩胶 分离胶 分离胶I / 2 ml x n / 3 ml x n / 4 ml x n 分离胶II / 2 ml x n / 3 ml x n / 4 ml x n 浓缩胶I 1 ml x n / 1 ml x n / 1.5 ml x n / 浓缩胶II 1 ml x n / 1 ml x n / 1.5ml x n / TEMED 2 µl x n 2 µl x n 2 µl x n 3 µl x n 3 µl x n 4 µl x n 10% APS 20 µl x n 40 µl x n 20 µl x n 60 µl x n 30 µl x n 80 µl x n

ExCell 胎牛血清（标准级）500ml 适用于增殖旺盛的常规细胞系的培养。

3200.00元

本血源来自依科赛公司精心挑选的国内优质血源地，经过与国际知名品牌同样的3级100 nm过滤，符合《中华人民共和国药典》标准，具有极佳的促细胞生长效果，满足部分科研和工业生产的需要。适用于增殖旺盛的常规细胞系的培养 产品特点：精选国内血源国际一流GMP生产线产品符合《中华人民共和国药典》标准内毒素含量≤10EU/ml; 总蛋白含量≤5.0%(w/v)适用于增殖旺盛的常规细胞系的培养

ExCell(SERUM), FSS500, Fetal Bovine Serum ( Standard) 500ml

3000.00元

EXCELL(SERUM)，FSD500，Fetal Bovine Serum(Defined)，500ml，胎牛血清

4800.00元

Fetal Bovine Serum (Defined ) 特级 胎牛血清

8600.00元

本产品血源地源自澳洲，并在澳洲加工生产，产品经3级100nm过滤，符合国际知名胎牛血清检测标准。适用于原代细胞、部分干细胞的培养。产品特点：精选澳洲血源正宗原装进口来自非疫区，国家检疫检验合格内毒素含量≤5EU/ml; 血红蛋白含量≤0.02%(w/v)适用于原代细胞、部分干细胞的培养

BioFroxx ，8211KG001， 琼脂粉 Agar

504.00元

中文品名：琼脂粉英文品名：Agar货号：8211CAS号：9002-18-0外观：乳白色粉末强度(1.5%)：800 - 1200 g/cm2储存条件：室温保质期：五年概述：琼脂粉是由琼脂糖和琼脂果胶组成的，溶解性是琼脂粉不溶于冷水，易溶于沸水，缓溶于热水。在生物学实验中，微生物培养基、植物组培培养基常规添加琼脂粉的用量1.5-2% （工作浓度6-25g/L）。当实验中遇到琼脂粉不凝固时，常规的检测方法时pH值，浓度，温度，充分混匀等情况。未凝结原因分析：培养基PH值过低 ，偏酸，水解，琼脂粉在PH值小于4则无法凝结；所加琼脂粉质量不够 ，增加用量。搅拌不均匀，倒板前没有充分摇匀，凝固不均匀；灭菌温度过高，加热时间过长。PH改变或破坏了琼脂的凝固度；培养基没有加热溶解充分，就没有凝固。（注：配置方法仅供参考，请根据具体实验要求结合文献进行使用）

BioFroxx， 1219GR100 ，L-脯氨酸L-Proline

204.00元

中文品名：L-脯氨酸英文品名：L-Proline货号：1219CAS号：147-85-3外观：无色结晶纯度：>99%储存条件：RT保质期：两年概述：L-脯氨酸是合成人体蛋白质的重要氨基酸之一，是氨基酸输液的重要原料，也是合成卡托普利、依那普利等一线降压药物的主要中间体，已被广泛应用于食品与医药等工业。配置方法：请根据具体实验要求结合文献进行使用

BioFroxx ，1219GR500， L-脯氨酸L-Proline

720.00元

中文品名：L-脯氨酸英文品名：L-Proline货号：1219CAS号：147-85-3外观：无色结晶纯度：>99%储存条件：RT保质期：两年概述：L-脯氨酸是合成人体蛋白质的重要氨基酸之一，是氨基酸输液的重要原料，也是合成卡托普利、依那普利等一线降压药物的主要中间体，已被广泛应用于食品与医药等工业。配置方法：请根据具体实验要求结合文献进行使用

BioFroxx ，1210GR025， L-谷氨酰胺L-Glutamine

60.00元

中文品名：L-谷氨酰胺英文品名：L-Glutamine货号：1210CAS号：56-85-9外观：无色针状结晶纯度：>99%储存条件：RT保质期：三年概述：L-谷氨酰胺在食品加工中作营养增补剂，调香增补剂。L-谷氨酰胺可作为培养细胞的能量来源、参与蛋白质的合成和核酸代谢。L-谷氨酰胺在溶液中经过一段时间后会降解，但是确切的降解率一直没有确定。L-谷氨酰胺的降解导致氨的形成，而氨对于一些细胞具有毒性。L-谷氨酰胺是细胞合成核酸和蛋白质的重要原材料，在细胞培养中，谷氨酰胺缺乏会导致细胞生长不良甚至死亡。在配制多数细胞培养液时都需补加一定量的谷氨酰胺。由于谷氨酰胺在溶液中很不稳定，故应单独配制，置于-20℃ 冰箱中保存，用前加入培养液中。加有谷氨酰胺的培养液在4℃冰箱中储存2周以上时，应重新补加谷氨酰胺。配置方法：谷氨酰胺2.922g溶于三蒸水，加至100ml，即配成200mmol/l的溶液。使用时可向100ml培养液中加入0.5-2ml谷氨酰胺浓缩液，使其终浓度为1-4mmol/l。（注：配置方法仅供参考，请根据具体实验要求结合文献进行使用）

BioFroxx ，1210GR100， L-谷氨酰胺L-Glutamine

187.00元

中文品名：L-谷氨酰胺英文品名：L-Glutamine货号：1210CAS号：56-85-9外观：无色针状结晶纯度：>99%储存条件：RT保质期：三年概述：L-谷氨酰胺在食品加工中作营养增补剂，调香增补剂。L-谷氨酰胺可作为培养细胞的能量来源、参与蛋白质的合成和核酸代谢。L-谷氨酰胺在溶液中经过一段时间后会降解，但是确切的降解率一直没有确定。L-谷氨酰胺的降解导致氨的形成，而氨对于一些细胞具有毒性。L-谷氨酰胺是细胞合成核酸和蛋白质的重要原材料，在细胞培养中，谷氨酰胺缺乏会导致细胞生长不良甚至死亡。在配制多数细胞培养液时都需补加一定量的谷氨酰胺。由于谷氨酰胺在溶液中很不稳定，故应单独配制，置于-20℃ 冰箱中保存，用前加入培养液中。加有谷氨酰胺的培养液在4℃冰箱中储存2周以上时，应重新补加谷氨酰胺。配置方法：谷氨酰胺2.922g溶于三蒸水，加至100ml，即配成200mmol/l的溶液。使用时可向100ml培养液中加入0.5-2ml谷氨酰胺浓缩液，使其终浓度为1-4mmol/l。（注：配置方法仅供参考，请根据具体实验要求结合文献进行使用）