
安全信息符号信号词危险声明警示性声明危害码(欧洲)风险声明(欧洲)安全声明(欧洲)RIDADRNONH for all modes of transportWGK德国RTECS闪点(华氏)闪点(摄氏)

产品特性级别low dust描述anionic detergent含量≥99.5%摩尔质量288.38 g/mol应用HPLC: suitable熔点204-207 °C (lit.)应用阴离子去垢剂十二烷基硫酸钠作为一种测试化合物,用于研究解淀粉芽孢杆菌KPS46是否可诱导大豆(Glycine max L.)反抗几种疾病的启动、信号传导、蛋白质生产以及系统保护。生物化学/生理学行为十二烷基硫酸钠(SDS)是通过疏水作用与蛋白质形成复合物的阴离子去垢剂。 研究表明蛋白质 - SDS复合物的流体动力学功能取决于他们多肽链的长度。 因此,基于SDS的电泳技术可以基于其分子量分离蛋白质分子。此外,SDS可以溶解细胞膜,并且可以提取膜结合蛋白。分析注意事项针对用于变性聚丙烯酰胺凝胶电泳进行了测试。

产品特性级别low dust描述anionic detergent含量≥99.5%摩尔质量288.38 g/mol应用HPLC: suitable熔点204-207 °C (lit.)应用阴离子去垢剂十二烷基硫酸钠作为一种测试化合物,用于研究解淀粉芽孢杆菌KPS46是否可诱导大豆(Glycine max L.)反抗几种疾病的启动、信号传导、蛋白质生产以及系统保护。生物化学/生理学行为十二烷基硫酸钠(SDS)是通过疏水作用与蛋白质形成复合物的阴离子去垢剂。 研究表明蛋白质 - SDS复合物的流体动力学功能取决于他们多肽链的长度。 因此,基于SDS的电泳技术可以基于其分子量分离蛋白质分子。此外,SDS可以溶解细胞膜,并且可以提取膜结合蛋白。分析注意事项针对用于变性聚丙烯酰胺凝胶电泳进行了测试。

产品特性pH值5 - 7特色行业Agriculture灰分<=0.1 %级别for molecular biology痕量阳离子Fe: <=1 ppm熔点118 - 121 °C(lit.)含量>=99.5%杂质<=0.3% water外源活性位点DNase, RNase, none detected储存温度常温应用离液剂和强变性剂;溶解细胞。

产品特性可溶性H2O: soluble6 M, clear (100 Hazen)熔点180-185°C(lit.)含量>=99.5%应用强离液剂,可用于蛋白质的变性和随后的复性。 这种强变性剂可以溶解不溶性或变性的蛋白质,例如包涵体。 这可以用作将蛋白质或酶重折叠成其活性形式的第一步。 尿素和二硫苏糖醇(DTT)也可能是必需的。

产品特性可溶性H2O: soluble6 M, clear (100 Hazen)熔点180-185°C(lit.)含量>=99.5%应用强离液剂,可用于蛋白质的变性和随后的复性。 这种强变性剂可以溶解不溶性或变性的蛋白质,例如包涵体。 这可以用作将蛋白质或酶重折叠成其活性形式的第一步。 尿素和二硫苏糖醇(DTT)也可能是必需的。
0.2 mL PCR八联管(高管),自然色(含平盖),可以做荧光定量340元是403002+406012的各一包的价格

产品特性HLB15转变温度cloud point 65°C摩尔质量average mol wt 1310摩尔质量micellar avg mol wt 79,000CMC0.012 mM(20-25C)形态viscous liquid组成Oleic acid, >=58.0% (balance primarily linoleic, palmitic, and stearic acids)描述non-ionic聚集数60应用非离子型洗涤剂,用于从哺乳动物细胞系的细胞核中选择性提取和分离蛋白质。

产品特性HLB15转变温度cloud point 65°C摩尔质量average mol wt 1310摩尔质量micellar avg mol wt 79,000CMC0.012 mM(20-25C)形态viscous liquid组成Oleic acid, >=58.0% (balance primarily linoleic, palmitic, and stearic acids)描述non-ionic聚集数60应用非离子型洗涤剂,用于从哺乳动物细胞系的细胞核中选择性提取和分离蛋白质。

产品特性HLB15转变温度cloud point 65°C摩尔质量average mol wt 1310摩尔质量micellar avg mol wt 79,000CMC0.012 mM(20-25C)形态viscous liquid组成Oleic acid, >=58.0% (balance primarily linoleic, palmitic, and stearic acids)描述non-ionic聚集数60应用非离子型洗涤剂,用于从哺乳动物细胞系的细胞核中选择性提取和分离蛋白质。

产品简介:SDS-PAGE 上样缓冲液(SDS-PAGE Loading Buffer, 5X),是一种经过改良的以溴酚蓝为染料,5 倍浓缩的蛋白上样缓冲液,用于 SDS-PAGE 时蛋白样品的上样操作。不含传统的 β-巯基乙醇或 DTT,Genefist 上样缓冲液采用 TCEP 作为还原剂,稳定性更好、还原效果更优,并且无难闻气味!保存条件:-20℃保存,至少一年有效。使用说明:1、在室温或不超过 37℃的水浴中溶解蛋白上样缓冲液。水浴溶解后立即室温存放,尽量避免长时间置于水浴中。使用完毕后置于-20℃保存。2、按照每 4ul 蛋白样品加入 1ul 上样缓冲液的比例,混合蛋白样品和上样缓冲液。3、100℃或沸水浴加热 5-10 分钟,以充分变性蛋白。4、冷却到室温后,上样到 SDS-PAGE 胶加样孔内,开始电泳。注意事项: 由于含有变性剂,本品不适用于非变性胶的电泳 SDS-PAGE 蛋白上样缓冲液(5X)必须完全溶解后再使用。 如果起始时细胞或组织的用量较大,基因组 DNA 含量较高,“混合液”可适当延长加热时间。 本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗。

产品简介通用型抗体稀释液是一种即用型的,适合于稀释所有免疫抗体测定(Immunofluorescence, IHC, ELISA, WB)实验中的抗体。该试剂可用于一抗或二抗的稀释和配制,在 4℃保存和使用不少于六个月。稀释液中加入了多种抗体结合反应增强剂及稳定剂,能够增加抗体的溶解度和稳定性,增强免疫反应,降低非特异的背景颜色,提高实验的特异性和灵敏度,可反复使用,节约宝贵的抗体。试剂中不含有动物源的蛋白,适合于所有类型抗体稀释,包括 HRP,AP 标记的抗体。不含磷酸盐、叠氮钠或硫柳汞类防腐剂,安全性更高,。产品特色 即用型稀释液,无需配制 稀释后的抗体可以使用长达 6 个月 适用所有实验的抗体稀释(IF、IHC、ELISA、WB) 含抗体结合反应增强剂、稳定剂,效果更佳 不含 BSA、脱脂奶粉等动物源蛋白 不含磷酸盐、叠氮钠或硫柳汞类防腐剂运输和保存条件常温运输,4℃存储,有效期两年。操作步骤 : 根据抗体使用说明书,直接用本品对一抗或二抗进行适当倍数的稀释。 保存在抗体稀释液中的抗体可回收于 4℃保存,可反复使用多次,长达 6 个月以上,直至不能达到理想结果后丢弃。注意事项1、使用后请旋紧瓶盖,防止溶液挥发或与空气的物质发生化学反应,并放回 4℃保存。2、在进行抗体免疫反应前可以使用 Genefist 的高效 Western 封闭液(货号GF1815)进行封闭,能进一步提高条带的清晰度,减少显色后的高背景和杂带的产生 。3、本品不含叠氮钠或硫柳汞类防腐剂,但还需小心操作避免皮肤直接接触。4、本试剂只能用于科研实验,请勿用于临床诊断和其它用途。

产品简介通用型抗体稀释液是一种即用型的,适合于稀释所有免疫抗体测定(Immunofluorescence, IHC, ELISA, WB)实验中的抗体。该试剂可用于一抗或二抗的稀释和配制,在 4℃保存和使用不少于六个月。稀释液中加入了多种抗体结合反应增强剂及稳定剂,能够增加抗体的溶解度和稳定性,增强免疫反应,降低非特异的背景颜色,提高实验的特异性和灵敏度,可反复使用,节约宝贵的抗体。试剂中不含有动物源的蛋白,适合于所有类型抗体稀释,包括 HRP,AP 标记的抗体。不含磷酸盐、叠氮钠或硫柳汞类防腐剂,安全性更高,。产品特色 即用型稀释液,无需配制 稀释后的抗体可以使用长达 6 个月 适用所有实验的抗体稀释(IF、IHC、ELISA、WB) 含抗体结合反应增强剂、稳定剂,效果更佳 不含 BSA、脱脂奶粉等动物源蛋白 不含磷酸盐、叠氮钠或硫柳汞类防腐剂运输和保存条件常温运输,4℃存储,有效期两年。操作步骤 : 根据抗体使用说明书,直接用本品对一抗或二抗进行适当倍数的稀释。 保存在抗体稀释液中的抗体可回收于 4℃保存,可反复使用多次,长达 6 个月以上,直至不能达到理想结果后丢弃。注意事项1、使用后请旋紧瓶盖,防止溶液挥发或与空气的物质发生化学反应,并放回 4℃保存。2、在进行抗体免疫反应前可以使用 Genefist 的高效 Western 封闭液(货号GF1815)进行封闭,能进一步提高条带的清晰度,减少显色后的高背景和杂带的产生 。3、本品不含叠氮钠或硫柳汞类防腐剂,但还需小心操作避免皮肤直接接触。4、本试剂只能用于科研实验,请勿用于临床诊断和其它用途。

半干转膜系统快速转膜液(5X)Cat #. GF1816 / Size. 500 ml产品简介半干转膜系统快速转膜液 Western blot Rapid Transfer Buffer for semi-dry(5X)是 Genefist推出的一种 5 倍浓度的高效转膜液,能高效快速地将蛋白转移到印迹膜(PVDF 或硝酸纤维素膜)上。本转膜液使用过程中,无需再使用甲醇或乙醇等任何醇类试剂,且能在 5-15 分钟内完成转膜过程,快速、高效、安全!产品特色: 高效安全:不使用甲醇或乙醇等任何醇类试剂,减少有毒试剂使用; ˗ 快速稳定:能在 5-15 分钟完成转膜过程,产热量小,减少蛋白损失; 兼容性好:兼容 Laemmli 胶,预制胶,FastPAGE,Bis-Tris 胶等多种凝胶。运输和保存条件:常温运输,室温存储,有效期一年。操作步骤 :1、1X 快速转膜液的配制(100ml):取 20ml 快速转膜液(5×)+ 80ml 去离子水;2、做好转印三明治结构,然后转移至电泳槽中,设定恒压 20V,一般时间 5-15 分钟完成蛋白转膜。备注: PVDF 膜使用前要用无水甲醇润湿 30 秒以上。 转印电流建议设定恒压 20V,1mm 厚度的凝胶,一般 5 分钟可以将 150kD 以下蛋白完全转印;大于 150kD 蛋白 10-15 分钟即可完全转印。 凝胶的浓度影响转印的效率,对于胶浓度 7.5%,5-10 分钟即可完成转印;对于胶浓度 10%,,5-15 分钟即可完成转印;对于胶浓度大于 10%,建议延长至 8-20 分钟。注意事项1、使用本试剂前请仔细阅读说明书。2、本产品只能用于科研实验,请勿用于临床诊断及其它用途。

Product Information GeneFist ™series3-color High Range Protein Marker(9-245 kDa)GF6619Size:250 µl × 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6619 GeneFist™ 3-color High Range ProteinMarker is a ready to use three-color protein standardwith 12 pre-stained proteins covering a wide range ofmolecular weights from 10 to 245 kDa in Tris-Glycinebuffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins arecovalently coupled with different chromophores foreasy identification of bands, with two referenceproteins carrying enhanced intensity correspondingto a green at 25 kDa and red at 75 kDa, respectively,as separated on SDS-PAGE (Tris-Glycine buffer).The GF6619 GeneFist™ 3-color High Range ProteinMarker is designed for monitoring protein separationduring SDS-polyacrylamide gel electrophoresis,verification of Western transfer efficiency onmembranes (PVDF, nylon, or nitrocellulose) and forapproximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6619 GeneFist™ 3-color High Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue2452352352Blue1801701703Blue1401301304Blue10093935Red7570726Blue6053537Blue4541428Blue3530309Green25222310Blue20181811Blue15141412Blue109103-color High Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6619 GeneFist™ 3-color High Range Protein Marker resolves 12 majorbands in SDS-PAGE (Tris-Glycine, MOPS, and MESbuffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist 3-color Regular Range ProteinMarker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only

Product Information GeneFist ™series3-color High Range Protein Marker(9-245 kDa)GF6619Size:250 µl × 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6619 GeneFist™ 3-color High Range ProteinMarker is a ready to use three-color protein standardwith 12 pre-stained proteins covering a wide range ofmolecular weights from 10 to 245 kDa in Tris-Glycinebuffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins arecovalently coupled with different chromophores foreasy identification of bands, with two referenceproteins carrying enhanced intensity correspondingto a green at 25 kDa and red at 75 kDa, respectively,as separated on SDS-PAGE (Tris-Glycine buffer).The GF6619 GeneFist™ 3-color High Range ProteinMarker is designed for monitoring protein separationduring SDS-polyacrylamide gel electrophoresis,verification of Western transfer efficiency onmembranes (PVDF, nylon, or nitrocellulose) and forapproximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6619 GeneFist™ 3-color High Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue2452352352Blue1801701703Blue1401301304Blue10093935Red7570726Blue6053537Blue4541428Blue3530309Green25222310Blue20181811Blue15141412Blue109103-color High Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6619 GeneFist™ 3-color High Range Protein Marker resolves 12 majorbands in SDS-PAGE (Tris-Glycine, MOPS, and MESbuffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist 3-color Regular Range ProteinMarker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only

Product Information GeneFist ™ series3-color Regular Range Protein Marker(9-180 kDa)GF6616Size:250 µl x 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6616 GeneFist™ 3-color Regular RangeProtein Marker is a ready to use three-color proteinstandard with 10 pre-stained proteins covering a widerange of molecular weights from 10 to 180 kDa (9 to170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDain Bis-Tris (MES) buffer). Proteins are covalentlycoupled with a blue chromophore except for tworeference bands (one green and one red band at 25kDa and 75 kDa respectively) when separated on SDSPAGE (Tris-Glycine buffer).The GF6616 GeneFist™ 3-color Regular RangeProtein Marker is designed for monitoring proteinseparation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiencyon membranes, (PVDF, nylon, or nitrocellulose) andfor approximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6616 GeneFist™ 3-color Regular Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue1801701702Blue1401301303Blue10093934Red7570725Blue6053536Blue4541427Blue3530308Green2522239Blue15141410Blue109103-color Regular Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6616 GeneFist™3-color Regular Range Protein Marker resolves 10major bands in SDS-PAGE (Tris-Glycine, MOPS, andMES buffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist Enhanced 3-color Regular RangeProtein Marker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only

Product Information GeneFist ™ series3-color Regular Range Protein Marker(9-180 kDa)GF6616Size:250 µl x 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6616 GeneFist™ 3-color Regular RangeProtein Marker is a ready to use three-color proteinstandard with 10 pre-stained proteins covering a widerange of molecular weights from 10 to 180 kDa (9 to170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDain Bis-Tris (MES) buffer). Proteins are covalentlycoupled with a blue chromophore except for tworeference bands (one green and one red band at 25kDa and 75 kDa respectively) when separated on SDSPAGE (Tris-Glycine buffer).The GF6616 GeneFist™ 3-color Regular RangeProtein Marker is designed for monitoring proteinseparation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiencyon membranes, (PVDF, nylon, or nitrocellulose) andfor approximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6616 GeneFist™ 3-color Regular Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue1801701702Blue1401301303Blue10093934Red7570725Blue6053536Blue4541427Blue3530308Green2522239Blue15141410Blue109103-color Regular Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6616 GeneFist™3-color Regular Range Protein Marker resolves 10major bands in SDS-PAGE (Tris-Glycine, MOPS, andMES buffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist Enhanced 3-color Regular RangeProtein Marker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only