中文 
bs-1353R，bioss，Bcl-2同源结构域蛋白抗体 Beclin 1
基本售价：980.00元
产品编号bs-1353R英文名称Beclin 1中文名称Bcl-2同源结构域蛋白抗体别 名Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like protein 1; 4921513J16Rik; 5430417M23Rik; ATG6; BECLIN 1; BECN1; MGC6843; BECN1_HUMAN. Specific References (14) | bs-1353R has been referenced in 14 publications.[IF=5.23] Chen, Cheng-Hsien, et al. "Far-infrared protects vascular endothelial cells from advanced glycation end products-induced injury via PLZF-mediated autophagy in diabetic mice." Scientific Reports 7 (2017): 40442. IHC-P ; Mouse. PubMed:28071754 [IF=5.17] Zhang et al. P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis. (2015) Oncotarge. 6:789-801 WB ; Human. PubMed:25596736 [IF=5.17] Zhang et al. P62 regulates resveratrol-mediated Fas/Cav-1 complex formation and transition from autophagy to apoptosis. (2015) Oncotarge. 6:789-801 WB ; Human. PubMed:25596736 [IF=4.3] Wang, Jinheng, et al. "Bcl-3, induced by Tax and HTLV-1, inhibits NF-κB activation and promotes autophagy." Cellular Signalling (2013). WB ; Human. PubMed:24044922 [IF=4.2] Yang, Huan, et al. "Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats." Stem Cell Research & Therapy 8.1 (2017): 267. WB ; Human. PubMed:29157289 [IF=3.23] Sun, Qianqian, et al. "Factors that Affect Pancreatic Islet Cell Autophagy in Adult Rats: Evaluation of a Calorie-Restricted Diet and a High-Fat Diet."PLOS ONE 11.3 (2016): e0151104. IHC-P ; Rat. PubMed:26963814 [IF=3.04] Shi C et al. Acetaminophen aggravates fat accumulation in NAFLD by inhibiting autophagy via the AMPK/mTOR pathway. European Journal of Pharmacology.2019. WB ; Mouse&Human. PubMed:10.1016/j.ejphar.2019.02.005 [IF=2.78] Chen, Yunbo, et al. "β-Asarone prevents autophagy and synaptic loss by reducing ROCK expression in asenescence-accelerated prone 8 mice." Brain research 1552 (2014): 41-54. WB ; Mouse. PubMed:24457043 [IF=2.33] Tang, Qishan, et al. "Resveratrol-induced apoptosis is enhanced by inhibition of autophagy in esophageal squamous cell carcinoma." Cancer letters 336.2 (2013): 325-337. WB ; Human. PubMed:23541682 [IF=1.5] Wang, Fang-Li Ma, Ning Tao, and Zhi-Hai Qin. "Polysaccharide from Polygonatum Inhibits the Proliferation of Prostate Cancer-Associated Fibroblasts Cells." Asian Pacific Journal of Cancer Prevention 17.8 (2016): 3827-3831. WB ; Human. PubMed:27644624 [IF=1.39] Tian et al. Role of endoplasmic reticulum stress on cisplatin resistance in ovarian carcinoma. (2017) Oncol.Let. 13:1437-1443 WB ; Human. PubMed:28454274 [IF=.18] Liu, B., et al. "Autophagy activation aggravates neuronal injury in the hippocampus of vascular dementia rats." Neural Regeneration Research 9.13 (2014): 1288. IHC-P ; Rat. PubMed:25221581 [IF=0] Tian, Jing, Rong Liu, and Quanxin Qu. "Role of endoplasmic reticulum stress on cisplatin resistance in ovarian carcinoma." Oncology Letters. WB ; Human. PubMed:28454274 [IF=3.067] Kang Z et al. Copper-induced apoptosis and autophagy through oxidative stress-mediated mitochondrial dysfunction in male germ cells. Toxicol In Vitro. 2019 Sep 3;61:104639. WB ; Mouse. PubMed:31491480 研究领域肿瘤 免疫学 神经生物学 细胞凋亡 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, 产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量50kDa细胞定位细胞浆 细胞膜 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human BECN1:201-330/450 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 52kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons.Function:Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.Subunit:Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB. Interacts with DAPK1. Interacts with human cytomegalovirus/HHV-5 protein TRS1.Subcellular Location:Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.Tissue Specificity:Ubiquitous.Post-translational modifications:Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.Ubiquitinated. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes.Similarity:Belongs to the beclin family.SWISS:Q14457Gene ID:8678Database links:Entrez Gene: 8678 HumanEntrez Gene: 56208 MouseEntrez Gene: 114558 RatOmim: 604378 HumanSwissProt: Q14457 HumanSwissProt: O88597 MouseSwissProt: Q91XJ1 RatUnigene: 716464 HumanUnigene: 178947 MouseUnigene: 2776 RatImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.BECN1是一种分子质量为60KDa的蛋白质, 它能与细胞凋亡抑制剂Bcl-2相互作用, 减少中枢神经系统的凋亡，参与PI3K复合物的组成和自噬体的形成。主要表达在胞质。抑癌基因beclin 1通过自噬作用来调节细胞生长。产品图片Sample: Small intestine(Mouse) lysate at 30ug;Primary: Anti-Beclin-1 (bs-1353R) at 1:200 dilution;Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;Predicted band size : 50kDObserved band size : 50kDParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (bs-1353R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩� for 20 min;Incubation: Anti-Beclin-1 Polyclonal Antibody, Unconjugated(bs-1353R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Beclin 1) polyclonal Antibody, Unconjugated (bs-1353R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Beclin 1) polyclonal Antibody, Unconjugated (bs-1353R ) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Tissue/cell: rat stomach tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Beclin-1 Polyclonal Antibody, Unconjugated(bs-1353R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nucleiTissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Beclin-1 Polyclonal Antibody, Unconjugated(bs-1353R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
bs-4511R，bioss，微管蛋白β tubulin/Tubulin β（内参）抗体 Beta tubulin(Loading Control)
基本售价：680.00元
产品编号 bs-4511R英文名称 Beta tubulin中文名称 微管蛋白β tubulin/Tubulin β抗体别 名 Beta 4 tubulin; Tubulin-beta; Tubulin beta; Beta 5 tubulin; BetaTubulin; Beta-Tubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; Tubulin beta-5 chain; TUBB4A; TUBB4; Tubulin 5 beta; Tubulin beta-4 chain; TBB4A_HUMAN; Tubulin beta-4A chain.
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