中文 
Caspase 1活性检测试剂盒(分光光度法)
基本售价：500.00元
Caspase 1 Activity Assay Kit(Colorimetric Method)中文名称:Caspase 1活性检测试剂盒(分光光度法)货号:E-CK-A381检测原理 Caspase 1也称interkeukin 1β conberting enzyme (ICE)，是caspase家族中唯一可以剪切IL-1β前体蛋白或IL-18前体产生相应成熟的细胞因子的caspase。Caspase 11可以剪切45 kD的caspase 1前体蛋白，产生20 kD和10 kD的片段，这两个片段可以形成异源二聚体 (heterodimer)，并进一步由两个异源二聚体形成四聚体。Caspase 1可以通过剪切Bcl-XL来调节细胞凋亡，并通过剪切细胞因子的前体来调节相关的免疫反应，也可以通过水解Gastermin D (GSDMD)来调节细胞焦亡。本caspase 1活性检测试剂盒是基于caspase 1可以催化底物Ac-YVAD-pNA产生黄色的pNA (p-nitroaniline)，pNA在405 nm附近有强吸收，从而可以通过测定吸光度来检测caspase 1的活性。基本信息 应用类型细胞凋亡-Caspase检测类型定性分析或定量分析检测方法比色法样本类型细胞，组织检测时间4-6 hours检测仪器分光光度计自备试剂PBS(E-BC-R187)，Bradford蛋白测定试剂盒(E-BC-K168-S或E-BC-K168-M)保存温度-20℃，避光运输条件冰袋保质期6 months
人二胺氧化酶(DAO)酶联免疫吸附测定试剂盒
基本售价：3200.00元
产品货号:E-EL-H1241c 中文名:人二胺氧化酶(DAO)酶联免疫吸附测定试剂盒别称:AOC1, ABP1, Amiloride Binding Protein 1, Amine Oxidase(Copper-Containing)用途 该试剂盒用于体外定量检测Human 血清，血浆或其他生物体液中DAO浓度检测原理 本试剂盒采用双抗体夹心ELISA法。用抗人DAO抗体包被于酶标板上，实验时样品或标准品中的人DAO会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗人DAO抗体和辣根过氧化物酶标记的亲和素。抗人DAO抗体与结合在包被抗体上的人DAO结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，DAO浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中DAO的浓度。反应类型 夹心法规格 96T反应时间 3.5h反应性 Human检测方法 Colormetric检测范围 3.13—200 ng/mL灵敏度 1.88 ng/mL样本体积 100μL样本类型 血清，血浆或其他生物体液特异性 可检测样本中的人DAO，且与其它相关蛋白无明显交叉反应。重复性 板内，板间变异系数均<10%。典型数据 由于实验操作条件的不同（如操作者、移液技术、洗板技术和稳定条件等），标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。(ng/mL) O.D Average Corrected 2002.4072.4412.4242.3351001.5471.6051.5761.487500.9180.8960.9070.817250.4660.4840.4750.38612.50.280.2780.2790.196.250.1980.1680.1830.0943.130.1290.1470.1380.04900.0870.090.089--精密度板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(ng/mL) 10.7428.2690.3810.9527.5989.37Standarddeviation 0.681.293.070.641.433.93C V (%) 6.334.563.405.845.184.40回收率 分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 92-10698EDTA plasma (n=8) 85-10092Cell culture media (n=8) 89-10295线性 分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)94-10789-10195-109Average (%)99941011:4Range (%)87-10385-10088-103Average (%)9491951:8Range (%)88-10586-10088-104Average (%)9692951:16Range (%)89-10182-9682-96Average (%)948888试剂盒组成及保存 未拆封的试剂盒可在2-8℃保存一周；如果一周以后才使用试剂盒，请拆开试剂盒按照下表中的条件分别保存各组分。中文名称 英文名称 规格 保存条件 ELISA酶标板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 条-20℃，可存放6个月冻干标准品Reference Standard2 支浓缩生物素化抗体(100×)Concentrated Biotinylated Detection Ab1支 120 μL浓缩HRP酶结合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6个月标准品&样品稀释液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6个月生物素化抗体稀释液Biotinylated Detection Ab Diluent1瓶 14 mL酶结合物稀释液HRP Conjugate Diluent1瓶 14 mL浓缩洗涤液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反应终止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 张产品说明书Product Description1 份质检报告Certificate of Analysis1 份说明：所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些，请在使用时量取而非直接倒出。试验所需自备物品 1. 酶标仪(450nm波长滤光片)2. 高精度移液器，EP管及一次性吸头：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒温箱，双蒸水或去离子水4. 吸水纸
即用型正常山羊血清
基本售价：40.00元
产品货号:E-IR-R110 中文名:即用型正常山羊血清别称:山羊血清,Normal Goat,封闭液，IHC封闭液，IF封闭液产品简介 本品为正常山羊血清加入稳定剂，作为封闭液可效减少IHC/IF实验的非特异染色从而降低背景。 产品组成 产品编号 产品名称 5 mL 10 mL 50 mL Storage E-IR-R110 Normal Goat Blocking Buffer (Ready-to-Use) 5 mL 10 mL 50 mL 2~8°C 产品使用说明 本产品为即用型山羊血清，可直接使用。 作为对照IgG使用时，用量和其它IgG的用量应相同。对照IgG用量过多可能会导致背景过高。 作为免疫组化封闭液，请直接滴加到组织切片上进行封闭。 保存条件 2~8°C保存，有效期12个月。 注意事项 1.本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。2.为了您的安全和健康，请穿实验服并戴一次性手套操作。
Recombinant Human FGF-10(Cat. No.:CR11)
基本售价：14400.00元
Recombinant Human FGF-10(Cat. No.:CR11) 该产品共有2篇文献引用 产品描述Recombinant Human Fibroblast Growth Factor 10 is produced by our E.coli expression system and the target gene encoding Gln38-Ser208 is expressed.蛋白编号O15520 产品别称Fibroblast growth factor 10;FGF-10;Keratinocyte growth factor 2;FGF10;KGF-2;KGF2分子量19.5 KDa表观分子量19-22 KDa, reducing conditions剂型描述Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 8.0.内毒素Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.纯度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. 纯度-SEC-HPLCGreater than 95% as determined by SEC-HPLC. (Regularly tested) 生物活性-Cell Based AssayMouse Gastric organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , R-spondin 1 (Cat#CX83) and FGF-10 (Cat#CR11). The organoids showed good morphology. Mouse bile duct organoids were cultured with EGF (Cat#C029) , R-spondin 1 (Cat#CX83) ,FGF-10 (Cat#CR11) and HGF (Cat#CJ72). The organoids showed good morphology. 复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.运输条件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.产品背景Fibroblast growth factor 10 (FGF-10, KGF-2), is a member of the fibroblast growth factor (FGF) family that includes FGF-3, -7, and -22. KGF-2 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGFR2 and interacts more weakly with FGFR1. It plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. It exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 is required for normal branching morphogenesis. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.仅供科研或生产使用，不可直接应用于人体。
Recombinant Human RSPO1 (C-6His)(Cat. No.:CX83)
基本售价：17600.00元
Recombinant Human RSPO1 (C-6His)(Cat. No.:CX83) 该产品共有5篇文献引用 产品描述Recombinant Human R-spondin-1 is produced by our Mammalian expression system and the target gene encoding Ser21-Ala263 is expressed with a 6His tag at the C-terminus.蛋白编号Q2MKA7 产品别称RSPO1; R-spondin1; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO Rspo1; R-spondin; Rspondin; RP23-325M14.2; Roof plate-specific spondin-1分子量27.8 KDa表观分子量40 KDa, reducing conditions剂型描述Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.内毒素Less than 0.01 EU/µg as determined by LAL test.纯度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. 生物活性-Cell Based AssayMeasured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 4.06 ng/ml. Mouse intestinal organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) and R-spondin 1 (Cat#CX83). After five days of culture, the organoids showed good morphology and germination rate. Mouse Colon organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , and R-spondin 1 (Cat#CX83). The organoids showed good morphology. Mouse Gastric organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , R-spondin 1 (Cat#CX83) and FGF-10 (Cat#CR11). The organoids showed good morphology. Mouse bile duct organoids were cultured with EGF (Cat#C029) , R-spondin 1 (Cat#CX83) ,FGF-10 (Cat#CR11) and HGF (Cat#CJ72). The organoids showed good morphology. Human intestinal organoids (ipsc source) were cultured with Activin A(Cat#C687) , BMP-4(sample) , EGF (Cat#C029) , Wnt3a (Cat#C22R), Noggin (Cat#C028), R-spondin 1 (Cat#CX83) and FGF-4 (Cat#CR08) . The organoids showed good morphology. Human endometrial epithelial organoids were cultured with EGF (Cat#CH28) and R-spondin 1 (Cat#CX83). The organoids showed good morphology. Data were kindly provided by Novoprotein's customer. Thanks for her contribution. 复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.运输条件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.产品背景RSPO1 is a secreted protein,containing 2 FU(furin-like) repeats and 1 TSP type-1 domain and belonging to the R-spondin family. RSPO1 is required for the early development of gonads, regardless of sex. It has been found in mice only eleven days after fertilization. To induce cell proliferation, it acts synergistically with WNT4. They help stabilize β catenin, which activates downstream targets. RSPO1 is necessary in female sex development. It augments the WNT/β catenin pathway to oppose male sex development. In critical gonadal stages, between six and nine weeks after fertilization, the ovaries upregulate it while the testes downregulate it. RSPO1 can potentially aid in the treatment of mucositis, which is characterized by inflammation of the oral cavity. This unfortunate condition often accompanies chemotherapy and radiation in cancer patients with head and neck tumors.仅供科研或生产使用，不可直接应用于人体。
Recombinant Human KGF(Cat. No.:CH73)
基本售价：19200.00元
Recombinant Human KGF(Cat. No.:CH73) 该产品共有1篇文献引用 产品描述Recombinant Human Fibroblast Growth Factor 7/Keratinocyte Growth Factor is produced by our E.coli expression system and the target gene encoding Cys32-Thr194 is expressed.蛋白编号P21781 产品别称Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF分子量18.9 KDa表观分子量17 KDa, reducing conditions剂型描述Lyophilized from a 0.2 μm filtered solution of 20mM Tris，1mM EDTA，5% Trehalose, 0.02% Tween 80, pH 8.0.内毒素Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.纯度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. (QC verified) 纯度-SEC-HPLCGreater than 95% as determined by SEC-HPLC. (Regularly tested) 复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.运输条件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.产品背景Fibroblast growth factor 7 (FGF7) is a secreted protein which is mainly located in epithelial cells and belongs to the heparin-binding growth factors family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. It is possible major paracrine effector of normal epithelial cell proliferation.仅供科研或生产使用，不可直接应用于人体。
Recombinant Human PECAM-1 (C-6His)(Cat. No.:CS74)
基本售价：400.00元
Recombinant Human PECAM-1 (C-6His)(Cat. No.:CS74) 产品描述Recombinant Human Platelet Endothelial Cell Adhesion Molecule is produced by our Mammalian expression system and the target gene encoding Gln28Lys601 is expressed with a 6His tag at the C-terminus.蛋白编号AAH51822.1 产品别称Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA; PECA1; CD31; PECAM1分子量65.3 KDa表观分子量75-110 KDa, reducing conditions剂型描述Lyophilized from a 0.2 μm filtered solution of PBS, 1mM EDTA, pH 7.4.内毒素 Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.运输条件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.产品背景Platelet Endothelial Cell Adhesion Molecule (PECAM1, CD31), is a heavily glycosylated transmembrane protein belonging to the immunoglobulin (Ig) superfamily of cell adhesion molecules. CD31 is composed of an extracellular domain (ECD) of 574 amino acids (aa) containing six Ig-like domains, a transmembrane domain, and a 118 aa cytoplasmic domain. CD31 is highly expressed on endothelial cells and at a lower level on platelets, granulocytes, macrophages, dendritic cells, T and B cells, and natural killer (NK) cells. It is involved in cell adhesion and is required for transepithelial migration of leukocytes (TEM). CD31 acts as a homophilic receptor through its extracellular domain and is involved in downstream signaling via its cytoplasmic domain. This domain contains highly conserved ITIM motifs which, once tyrosine phosphorylated, recruit and activate the signaling molecules Src and SHP2. The resulting inhibition of TCR signaling increases the activation threshold of T cells, thus reinforcing peripheral tolerance and preventing development of autoimmunity. CD31 additionally regulates immune responses by acting as a key inhibitory receptor in dendritic cell development. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells.仅供科研或生产使用，不可直接应用于人体。
Recombinant Mouse IL-4 (C-6His)(Cat. No.:CK74)
基本售价：17600.00元
Recombinant Mouse IL-4 (C-6His)(Cat. No.:CK74) 该产品共有24篇文献引用 产品描述Recombinant Mouse Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His21-Ser140 is expressed with a 6His tag at the C-terminus.蛋白编号P07750 产品别称Interleukin-4; IL-4; IL4; B-cell IgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1; BSF-1; IGG1 induction factor; Lymphocyte stimulatory factor 1分子量14.6 KDa表观分子量15-19 KDa, reducing conditions剂型描述Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.内毒素 Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.运输条件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.产品背景Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.仅供科研或生产使用，不可直接应用于人体。
NovoStart SYBR qPCR SuperMix Plus试剂
基本售价：1280.00元
NovoStart SYBR qPCR SuperMix Plus试剂 该产品共有552篇文献引用 产品描述本制品是采用SYBR Green I嵌合荧光法进行Real Time PCR的专用试剂。预混液中已经将DNA聚合酶、精心优化的反应Buffer、dNTPs、SYBR Green I等试剂预混在一起，是一种2×浓度的预混型试剂，进行实验时，PCR反应液的配制十分简单方便。本制品使用新型抗体修饰的HotStart Taq DNA聚合酶，并结合精心优化的反应Buffer，从而有效抑制低温下的非特异性扩增，提高反应特异性和扩增效率，能够在更宽广的范围内进行准确定量。产品特点高特异性：采用新型工艺制备抗体修饰的HotStart Taq DNA聚合酶，无需热启动即可进行PCR反应，大大提高PCR扩增的特异性。高效：Novoprotein精心配制的RealTime PCR专用2×SuperMix，具有更高的扩增效率和扩增灵敏度。快捷：PCR反应所必需试剂集于一管之中，数分钟即可完成反应体系配制。保存条件-20℃避光储存。如需在一段时间内经常取用，可在2~8℃条件下储存3个月。避免反复多次冻融。质量控制纯度检测：所有组分经检测均无核酸内切酶残留、核酸外切酶残留、核酸残留。使用建议使用：请上下颠倒轻轻混合均匀，避免起泡，并经轻微离心后使用。反应液的配制、分装请使用新的（无污染的）枪头、Microtube等，避免污染。建议反应体积为20～50μl。引物设计：一般为了增加灵敏度及扩增效率且抑制非特异性反应，扩增目标序列越短越好。建议设计成200bp以下。仅供科研或生产使用，不可直接应用于人体。
Recombinant Mouse TNF alpha
基本售价：2520.00元
Recombinant Mouse TNF alpha 产品描述Recombinant Mouse Tumor Necrosis Factor Alpha is produced by our E.coli expression system and the target gene encoding Asp89-Leu235 is expressed.蛋白编号P06804 产品别称Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; Tumor Necrosis Factor; Membrane Form; Tumor Necrosis Factor; Soluble Form; Tnf; Tnfa; Tnfsf2分子量16.4 KDa表观分子量14 KDa, reducing conditions剂型描述Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.内毒素Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.纯度-SDS-PAGE
RNase R
基本售价：2000.00元
RNase R 产品描述核糖核酸酶R (Ribonuclease R，RNase R) 来源于大肠杆菌RNR超家族，是一种镁离子依赖性的3'—5'核糖核酸外切酶，可从3'—5'方向将RNA逐步切割成二核苷酸和三核苷酸。RNase R几乎能够消化所有线性的RNA，但不易消化环状RNA、套索结构RNA和3'端突出少于7个核苷酸的短双链RNA分子。 RNase R常用于基因表达和可变剪切研究，可消化线性RNA以富集环状RNA或套索结构RNA。保存条件-20±5℃仅供科研或生产使用，不可直接应用于人体。
Lysis dilution buffer（150 mM NaCl/5 mM EDTA）500ml
基本售价：170.00元
Lysis dilution buffer（150 mM NaCl/5 mM EDTA）500ml
TripleSelect无动物源常温胰酶
基本售价：260.00元
TripleSelect无动物源常温胰酶 【保存条件】 室温保存12个月, 2–8°C或–20°C保存24个月【概述】 TripleSelect与胰蛋白酶一样，可裂解赖氨酸和精氨酸C-末端上的肽键。但是，TripleSelect无与伦比的纯度提高了特异性，其原因在于只有一种酶发挥作用。这减少了胰蛋白酶和其他提取物中存在的多种酶的裂解作用造成的损害。【产品特点】 对细胞作用温和：纯度提高了特异性，其原因在于只有一种酶发挥作用。这减少了胰蛋白酶和其他提取物中由于多种酶切割造成的损害。在室温下保持稳定：在室温或4°C下可保持稳定12-24个月，因此易于储存和处理、方便快捷。无动物来源蛋白：与常用猪胰蛋白酶不同，本试剂不含动物来源成分。无需终止，易于使用：无需使用胰蛋白酶抑制剂（如FBS）终止消化，PBS或DMEM等稀释后自动失活。【使用建议】 1.使用前将TripleSelect和完整的生长培养基恢复至室温。2.将待消化细胞培养器皿中培养去除。3.使用DPBS（无钙镁）清洗细胞，清洗完去除DPBS液体。4.加入相应体积的TripleSelect消化液（如25cm2瓶中加入1-2ml TripleSelect消化液，75cm2瓶中加入5ml TripleSelect消化液）5.37°C孵育至细胞变圆脱落（镜下观察），可轻拍培养瓶/皿。6.加入5ml完全培养基至培养瓶/皿（体积根据不同面积培养瓶/皿），可轻轻吹打使细胞更好分离。7.将含细胞的液体移入15ml无菌离心管中。以200-500g转速离心5-10分钟使细胞沉淀。8.去除上清后，加入含血清的完全培养液使细胞沉淀重新悬浮，即可用于后续实验【注意事项】 1. 注意无菌操作，尽量避免污染。2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
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