中文 
105004，试管旋转混匀器
基本售价：1428.00元
下单前请联系客服询问货期，单位：箱试管旋转混匀器是一款设计独特的产品， 旋转速度温和，通过对试管板的调节，使样品处于任意角度旋转，广泛应用于分子生物学、化学、临床应用等各种需求混合的场合。可同时安装不同的试管板，并可调整不同的角度，以提高实验效率。• 体积小，操作简便；• 标配五种试管板，满足客户多种需求；• 开启状态下，轻按试管板，可反方向旋转；• 噪音低，运转平稳；• 高性能马达，使用寿命长。
105003，迷你漩涡混匀器
基本售价：686.00元
下单前请联系客服询问货期，单位：箱 迷你漩涡混匀器是专注于点动功能的试管振荡器，紧凑小巧的设计，触发式启动开关，安全便捷，是实验室必不可少的工具之一，可用于振荡试管、离心管，混合效果显著。• 结构小巧紧凑， 质量可靠；• 专用于振荡直径小于30mm的容器；• 工作垫片为耐磨材料制成，使用寿命长；• 精制钢材底座，运行稳定。
中科都菱-86/-150℃超低温保存箱系列 MDF-86V838E
基本售价：0.00元
高性价比自主研发的高效混合制冷剂配方,节能50%人性化设计采用新型门把手，可附加挂锁；配备测试孔设计；可调节的不锈钢搁架；内门可拆卸，便于客户清洁与除霜；报警方式箱内温度高低温报警，声光报警，可选配短信通知等报警方式；
中科都菱-86/-150℃超低温保存箱系列 MDF-86V688
基本售价：0.00元
特殊聚氨酯发泡保温材料-86系列产品内外温差可达110℃，特殊聚氨酯发泡保温层避免破裂及变形，150mm的保温材料厚度可确保内部温度的稳定性制冷系统双机复叠制冷系统，碳氢制冷剂节能50%特殊降噪结构每个可能产生噪音的部件都装有特殊材质的减震材料，机组周围装有高密度防火吸音棉
中科都菱-86/-150℃超低温保存箱系列 MDF-86V588E
基本售价：0.00元
产品特点自主研发的高效混合制冷剂配方,节能50%安全保障在恢复供电时，所有设备的同时启动会对电网造成较大冲击，从而可能导致断路器跳闸；针对这种情况特别设计的设备延时启动功能可使设备；人性化设计内门可拆卸，便于客户除霜与清洁；
中科都菱-86/-150℃超低温保存箱系列 MDF-86V408E
基本售价：0.00元
高性价比自主研发的高效混合制冷剂配方,节能50%人性化设计采用新型门把手，可附加挂锁；配备测试孔设计；可调节的不锈钢搁架；内门可拆卸，便于客户清洁与除霜；报警方式具备高低温报警、传感器故障报警、温控器故障报警、断电报警、门开报警、环温报警、冷凝器故障报警、过滤网检查报警、电压异常报警、电池电量低报警。
中科都菱-86/-150℃超低温保存箱系列 MDF-86V340E
基本售价：0.00元
高性价比自主研发的高效混合制冷剂配方,节能50%降温速度更快降温速度提升20%，均匀性提高30%单一制冷自复叠一个制冷回路，相比普通双级复叠系统减少一倍的系统部件
中科都菱-86/-150℃超低温保存箱系列 MDF-86V188E
基本售价：0.00元
高性价比自主研发的混合工质可使产品节能50%标配功能标配测试孔，USB接口以及远程报警接口报警方式箱内温度高低温报警，声光报警，可选配短信通知等报警方式；
中科都菱-86/-150℃超低温保存箱系列 MDF-86V50
基本售价：0.00元
采用优质冷轧钢板内壁为优质304不锈钢钢板，经久耐用，安全可靠高精度电脑控制系统精准保持箱体内部温度精确稳定，箱内温度-40~-86℃可调采用单级自复叠制冷系统制冷速度快，耗电量低，制冷量大
中科都菱-86/-150℃超低温保存箱系列 MDF-86H485
基本售价：0.00元
温控方式采用国际著名名牌高精度电脑温控系统，精准保持箱体内部温度精确稳定，箱内温度-40~-86℃可调；制冷系统采用优化的单级自复叠制冷系统设计，制冷速度快，耗电量低，制冷量大；安全保障具备高低温报警，传感器故障报警功能；具备声音蜂鸣和灯光闪烁报警方式；
中科都菱-86/-150℃超低温保存箱系列 MDF-86H105
基本售价：0.00元
箱体箱体外壳采用冷板喷涂；内胆为优质304不锈钢，经久耐用，安全可靠；温控方式采用全铜管蒸发器，精准的电子温度控制及显示，精度达到0.1℃；特色功能安全门锁设计；大屏幕数字显示便于观察；标配的测试孔；
METHER高端系列-86/-150℃超低温保存箱 MDF-86V588D（双系统）
基本售价：0.00元
双重保障采用两套独立的制冷系统，其中一套故障时，另一套可维持-70的柜内温度快速降温双系统同时工作能实现快速降温，降温时间缩短50%以上智能控温触摸屏，温度曲线、报警信息记录及查询，用户管理模式，自我诊断
METHER高端系列-86/-150℃超低温保存箱 MDF-86V728
基本售价：0.00元
特殊降噪音结构每个可能产生噪音的部件都装有特殊材质的减震材料，机组周围装有高密度防火吸音棉温度控制微电脑控制，箱内温度-40~-86℃可调，超温报警，断电记忆，调节精度为0.1℃；制冷系统优化的双机复叠式制冷系统，可以实现最高35%的节能效果
METHER高端系列-86/-150℃超低温保存箱 MDF-86V838
基本售价：0.00元
特殊降噪音结构每个可能产生噪音的部件都装有特殊材质的减震材料，机组周围装有高密度防火吸音棉温度控制微电脑控制，箱内温度-60~-86℃可调，超温报警，断电记忆，调节精度为0.1℃；制冷系统优化的双机复叠式制冷系统，可以实现最高35%的节能效果
METHER高端系列-86/-150℃超低温保存箱 MDF-86V588
基本售价：0.00元
制冷剂采用自主研发的无氟碳氢环保制冷剂VIPIIPLUS先进技术VIPIIPLUS第二代真空绝热板技术，使冰箱有良好的保温性能，专为进入电梯而设计产品特点专为生物样本库优化的产品结构，可以提供最大4万份样本的存储空间双机复叠技术，传统技术稳定性安全可靠，C/H制冷剂绿色无污染，更节能，第二代VIP技术，保温性能好，减小占地面积，样本储存量最优
METHER高端系列-86/-150℃超低温保存箱 MDF-86V338
基本售价：0.00元
显示面板屏幕尺寸为7英寸液晶触摸屏，可直观的进行操作及显示各种与设备相关的信息；报警系统具备高低温报警、传感器故障报警、冷凝器脏堵报警、环温异常报警、电压异常报警、断电报警、门开报警、电池电量低报警、远程报警接口，所有的报警信息以及历史记录可在液晶屏查询。USB数据存储功能具备USB数据存储功能，可存储10年以上的数据，方便查询历史记录；双机复叠技术，耐43℃环境温度，超保温
METHER高端系列-86/-150℃超低温保存箱 MDF-86V408
基本售价：0.00元
控温方式触控屏温度控制系统，控制更精确，温度数字与曲线双显示。温度-10~-86℃自由调节，满足不同物品不同温区存放，采用优质工业设备专用电阻式7英寸触控屏，防尘、防水汽双测试孔/温度打印标配双测试孔，USB接口以及远程报警接口，USB具有数据导出功能，配备热敏温度记录打印机，数据浏览功能可随时掌握运行周期状况：完善的声光报警功能高温、低温、开门、过滤网堵塞、系统故障、传感器故障、环温高报警等报警功能，确保箱内物品存放安全；密码保护功能系统，防止随意调整运行参数；双机复叠技术，传统技术稳定性安全可靠，C/H制冷剂绿色无污染，更节能，第二代VIP技术，保温性能好，减小占地面积，样本储存量最优
METHER高端系列-86/-150℃超低温保存箱 MDF-86V838D（双系统）
基本售价：0.00元
双重保障采用两套独立的制冷系统，其中一套故障时，另一套可维持-70℃的柜内温度快速降温双系统同时工作能实现快速降温，降温时间缩短50%以上智能控温触摸屏，温度曲线、报警信息记录及查询，用户管理模式，自我诊断
一氧化氮合成酶-2（诱导型）抗体 | RabbitAnti-iNOS antibody
基本售价：1098.00元
产品编号bs-0162R英文名称iNOS中文名称一氧化氮合成酶-2（诱导型）抗体别 名i NOS; Nitric Oxide Synthase, Inducible; HEP NOS; Hepatocyte NOS; HEPNOS; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; INOS; Inosl; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible macrophage; Nitric oxide synthase 2A (inducible hepatocytes); Nitric oxide synthase inducible; NOS 2; NOS 2A; NOS; Nos II; NOS type II; Nos2; NOS2A; NOS2_HUMAN. Specific References (47) | bs-0162R has been referenced in 47 publications.111 [IF=2.35] Li, Guofu, et al. "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." International Journal of Molecular Medicine 38.3 (2016): 767-775. WB ; Rat. 222111 [IF=4.56] Shanshan et al. Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury. (2017) Front.Cell.Neurosc. 11:99 WB ; Rat. 222PubMed:28424593 111 [IF=2.24] Zhang et al. High altitude increases the expression of hypoxia-inducible factor 1α and inducible nitric oxide synthase with intest-inal mucosal barrier failure in rats. (2015) Int.J.Clin.Exp.Patho. 8:5189-95 IHC ; Rat. 222PubMed:26191216 111 [IF=5.64] Wang et al. CXCL1 derived from tumor-associated macrophages promotes breast cancer metastasis via activating NF-κB/SOX4 signaling. (2018) Cell.Death.Dis. 9:880 WB ; Mouse. 222PubMed:30158589 111 [IF=2.8] Liu J et al. Alterations of antioxidant indexes and inflammatory cytokine expression aggravated hepatocellular apoptosis through mitochondrial and death receptor-dependent pathways in Gallus gallus exposed to arsenic and copper. Environ Sci Pollut Res Int WB ; Chicken. 222PubMed:29569195 111 [IF=2.332] Zhao X et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.(2018) Microb Pathog.125:431-437. WB ; Mouse. 222PubMed:30316005 111 [IF=2.655] Horváth E et al. Ischemic damage and early inflammatory infiltration are different in the core and penumbra lesions of rat brain after transient focal cerebral ischemia. J Neuroimmunol. 2018 Nov 15;324:35-42. IHC-P ; Rat. 222PubMed:30212790 111 [IF=4.183] Imoto K et al. Periostin Mediates Right Ventricular Failure through Induction of Inducible Nitric Oxide SynthaseExpression in Right Ventricular Fibroblasts from Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats. Int J Mol Sci. 2018 Dec 24;20(1). IHC-P ; Rat. 222PubMed:30586863 111 [IF=3.118] Lv R et al. Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway. Int Immunopharmacol. 2019 Feb 18;70:28-36. IHC-P ; Rat. 222PubMed:30785088 111 [IF=2.535] Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523. WB ; Human. 222PubMed:31383786 111 [IF=2.914] Guo MH et al. Elemental imbalance elicited by arsenic and copper exposure lead to oxidative stress and immunotoxicity in chicken gizzard, activating the protective effects of heat shock proteins. Environmental Science and Pollution Research. 2019. WB ; Chicken. 222111 [IF=3.87] Jiao F et al. AGK2 Alleviates Lipopolysaccharide Induced Neuroinflammation through Regulation of Mitogen-Activated Protein Kinase Phosphatase-1. J Neuroimmune Pharmacol. 2019 Nov 30. IHF ; Mouse. 222PubMed:31786712 111 [IF=3.483] Huang P et al. Theoretical basis of nitrosomyoglobin formation in a dry sausage model by coagulase-negative staphylococci: Behavior and expression of nitric oxide synthase. Meat Sci. 2019 Nov 27;161:108022. WB ; coagulase-negative staphylococci (CNS) and P. pentosaceus. 222PubMed:31838366 111 [IF=3.58] El-Lakany MA et al. Ovariectomy provokes inflammatory and cardiovascular effects of endotoxemia in rats: Dissimilar benefits of hormonal supplements. Toxicol Appl Pharmacol. 2020 Feb 21;393:114928. IHC-P ; Rat. 222PubMed:32092384 111 [IF=3.361] Wang M et al. Berberine combined with cyclosporine A alleviates acute graft-versus-host disease in murine models. Int Immunopharmacol. 2020 Feb 9;81:106205. IHC-P ; Mouse. 222PubMed:32050154 111 [IF=3.761] Toru Nishikawa et al. Therapeutic potential for insulin on type 1 diabetes‐associated periodontitis: analysis of experimental periodontitis in streptozotocin‐induced diabetic rats. J Diabetes Investig . 2020 Apr 17. IHC ; rat. 222PubMed:32302049 111 [IF=8.352] Ye Hea et al. Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation. Applied Materials Today 20 (2020) 100673 IHC/IF ; Rat/Mouse. 222111 [IF=2.5] Yang F et al. The effects of binge alcohol exposure on tooth movement and associated root resorption in rats. Alcohol . 2020 Nov;88:1-9. IHC-P ; Rat. 222PubMed:32574660 111 [IF=4.225] Jiang Q et al. Particulate Matter 2.5 Induced Developmental Cardiotoxicity in Chicken Embryo and Hatchling. Front Pharmacol. 2020 Jun 5;11:841. WB ; Chicken. 222PubMed:32581800 111 [IF=] WB ; mouse. 222111 [IF=7.919] Chen Y et al. A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activityClin Transl Med.2020 Dec;10(8):e240. WB、IHC ; Mouse. 222PubMed:33377656 111 [IF=7.727] Xue Wang. et al. Engineered liposomes targeting the gut–CNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:390 WB,IHC ; Mouse. 222111 [IF=3.909] Qian Xueshen. et al. Periodontitis Deteriorates Cognitive Function and Impairs Neurons and Glia in a Mouse Model of Alzheimer’s Disease. J Alzheimers Dis. 2021 Jan;79(4):1785-1800 WB ; Mouse. 222111 [IF=3.69] Xiaoyan Xia. et al. Ameliorative effect of white tea from 50-year-old tree of Camellia sinensis L. (Theaceae) on kidney damage in diabetic mice via SIRT1/AMPK pathway. J Ethnopharmacol. 2021 May;272:113919 WB ; Mouse. 222111 [IF=8.758] Yinying Sheng. et al. Development and In Vitro Biodegradation of Biomimetic Zwitterionic Phosphorylcholine Chitosan Coating on Zn1Mg Alloy. Acs Appl Mater Inter. 2020;12(49):54445–54458 IF ; Mouse. 222111 [IF=3.368] Liu Tang. et al. Topical Application with Conjugated Linoleic Acid Ameliorates 2, 4‐dinitrofluorobenzene‐Induced Atopic Dermatitis‐like Lesions in BALB/c Mice. Exp Dermatol. 2021 Feb;30(2):237-248 WB ; Mouse. 222111 [IF=2.965] Sawada Noritaka. et al. Glucagon-Like Peptide-1 Receptor Agonist Liraglutide Ameliorates the Development of Periodontitis. J Diabetes Res. 2020;2020:8843310 IHC ; Rat. 222111 [IF=5.88] Shuai Chen. et al. Chondroitin sulfate modified 3D porous electrospun nanofiber scaffolds promote cartilage regeneration. Mat Sci Eng C-Mater. 2021 Jan;118:111312 IHC ; Rabbit. 222111 [IF=8.724] Penghui Zhang. et al. Effect of cyclic mechanical loading on immunoinflammatory microenvironment in biofabricating hydroxyapatite scaffold for bone regeneration. Bioact Mater. 2021 Oct;6:3097 WB ; Mouse. 222111 [IF=3.943] Kai Kang. et al. FGF21 alleviates chronic inflammatory injury in the aging process through modulating polarization of macrophages. Int Immunopharmacol. 2021 Jul;96:107634 WB ; Mouse. 222研究领域细胞生物 免疫学 神经生物学 信号转导 转录调节因子 激酶和磷酸酶 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, 产品应用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.理论分子量130kDa细胞定位细胞核 细胞浆 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human NOS-2: 1051-1144/1144 亚 型IgG纯化方法affinity purified by Protein A缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.PubMedPubMed 产品介绍Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).Function:Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.Subunit:Homodimer. Binds SLC9A3R1.Tissue Specificity:Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.Similarity:Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.SWISS:P35228Gene ID:4843Database links:Entrez Gene: 4843 HumanEntrez Gene: 18126 MouseEntrez Gene: 24599 RatOmim: 163730 HumanSwissProt: P35228 HumanSwissProt: P29477 MouseSwissProt: Q06518 RatUnigene: 709191 HumanUnigene: 2893 MouseUnigene: 10400 Rat合成与降解（Synthesis and Degradation）催化生物体内一氧化氮（NO）生成的酶。分神经型一氧化氮合成的酶（nNOS or NOS-1）、诱导型一氧化氮合成的酶（iNOS or NOS-2）、内皮型一氧化氮合成的酶（eNOS or NOS-3）。产品图片Sample:U251 Cell Lysate at 40 ugPrimary: Anti-iNOS(bs-0162R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 130kDObserved band size: 130kDSample:Lane 1: Mouse Raw264.7 tissue lysatesLane 2: Human Jurkat cell lysatesPrimary: Anti-iNOS (bs-0162R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 130 kDaObserved band size: 140 kDaParaformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (inos) Polyclonal Antibody, Unconjugated (bs-0162R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (iNOS) Polyclonal Antibody, Unconjugated (bs-0162R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated(bs-0162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated(bs-0162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
缺氧诱导因子1α /低氧诱导因子-1抗体 | RabbitAnti-HIF-1 Alpha antibody
基本售价：1098.00元
产品编号bs-0737R英文名称HIF-1 Alpha中文名称缺氧诱导因子1α /低氧诱导因子-1抗体别 名ARNT interacting protein; ARNT-interacting protein; Basic helix loop helix PAS protein MOP1; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF 1alpha; HIF-1-alpha; HIF1 A; HIF1 Alpha; HIF1; HIF1-alpha; HIF1A; HIF1A_HUMAN; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor); Hypoxia inducible factor1alpha; Hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor-1a; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; MOP1; PAS domain-containing protein 8; PASD 8; PASD8. Specific References (22) | bs-0737R has been referenced in 22 publications.[IF=5.595] Liu T et al. MicroRNA-493 targets STMN-1 and promotes hypoxia-induced epithelial cell cycle arrest in G/M and renal fibrosis. (2018) FASEB J. Sep 05 WB ; Mouse. PubMed:30183377 [IF=5.08] Zhang, Huimin, et al. "Vascular Normalization Induced by Sinomenine Hydrochloride Results in Suppressed Mammary Tumor Growth and Metastasis." Scientific Reports 5 (2015). IHC-P ; Mouse. PubMed:25749075 [IF=4.91] Fan, Shengjun, et al. "Opposite angiogenic outcome of curcumin against ischemia and Lewis lung cancer models:in silico, in vitro and in vivo studies." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2014). WB ; Mouse. PubMed:24970744 [IF=4.556] Yang X et al. Synthesis and bioevaluation of novel 18FDG-conjugated 2-nitroimidazole derivatives for tumor-hypoxia imaging. Mol Pharm. 2019 May 6;16(5):2118-2128. IHC&IF ; Mouse&Human. PubMed:30964298 [IF=4.539] Talwar H et al. MEK2 Negatively Regulates Lipopolysaccharide-Mediated IL-1β Production through HIF-1α Expression. J Immunol. 2019 Mar 15;202(6):1815-1825. WB&IP ; Mouse. PubMed:30710049 [IF=4.44] Madka, Venkateshwar, et al. "Targeting mTOR and p53 signaling inhibits muscle invasive bladder cancer in vivo." Cancer Prevention Research 9.1 (2016): 53-62. IHC-P ; Mouse. PubMed:26577454 [IF=4.19] Talwar, Harvinder, et al. "MKP-1 negatively regulates LPS-mediated IL-1β production through p38 activation and HIF-1α expression." Cellular Signalling (2017). Mouse. PubMed:28238855 [IF=3.743] Wang D et al. Effects of hypoxia and ASIC3 on nucleus pulposus cells: From cell behavior to molecularmechanism. Biomed Pharmacother. 2019 Jun 12;117:109061. WB ; Rabbit. PubMed:31202172 [IF=3.53] Woolf, Eric C., et al. "The Ketogenic Diet Alters the Hypoxic Response and Affects Expression of Proteins Associated with Angiogenesis, Invasive Potential and Vascular Permeability in a Mouse Glioma Model." PLOS ONE10.6 (2015): e0130357. WB ; Mouse. PubMed:26083629 [IF=3.288] Chai D et al. β2-microglobulin has a different regulatory molecular mechanism between ER+ and ER- breast cancer with HER2.BMC Cancer. 2019 Mar 12;19(1):223. IHC-P ; Human. PubMed:30866857 [IF=2.989] Ju X et al. Catalpol Promotes the Survival and VEGF Secretion of Bone Marrow-Derived Stem Cells and Their Role in Myocardial Repair After Myocardial Infarction in Rats.Cardiovasc Toxicol. 2018 May 11. WB ; Rat. PubMed:29752623 [IF=2.91] Shou, Zhu, et al. "Expression and prognosis of FOXO3a and HIF-1?? in nasopharyngeal carcinoma."Journal of cancer research and clinical oncology 138.4 (2012): 585-593.. WB ; Human. PubMed:22209974 [IF=2.784] Yang D et al. Normobaric oxygen inhibits AQP4 and NHE1 expression in experimental focal ischemic stroke. (2018) Int. J. Mol. Med. WB ; Rat . PubMed:30592266 [IF=2.766] Dao DT et al. A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor.(2018) PLoS One. WB ; Mouse. PubMed:30566445 [IF=2.566] Yang Z et al. Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1ɑ axis in esophageal squamous cell carcinoma.Exp Mol Pathol. 2019 Mar 20. WB ; Human. PubMed:30904401 [IF=2.49] Yang, Ya, et al. "Expression of RAP1B is associated with poor prognosis and promotes an aggressive phenotype in gastric cancer." Oncology reports 34.5 (2015): 2385-2394. IHC-P ; Human. PubMed:26329876 [IF=1.64] Guo, Wei, et al. "Transplantation of endothelial progenitor cells in treating rats with IgA nephropathy." BMC Nephrology 15.1 (2014): 110. WB ; Rat. PubMed:25012471 [IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082. WB ; Rat. PubMed:27665780 [IF=1.41] Song et al. Effects of HSYA on the proliferation and apoptosis of MSCs exposed to hypoxic and serum deprivation conditions. (2018) Exp.Ther.Med. 15:5251-5260 WB ; Rat. PubMed:29904409 [IF=.5] Talwar, Harvinder, et al. "The dataset describes: HIF-1 α expression and LPS mediated cytokine production in MKP-1 deficient bone marrow derived murine macrophages." Data in Brief (2017). WB ; Mouse. PubMed:28765831 [IF=0] Talwar et al. The dataset describes: HIF-1 α expression and LPS mediated cytokine production in MKP-1 deficient bone marrow derived murine macrophages. (2017) Data.Brie. 14:56-61 WB ; Mouse. PubMed:28765831 [IF=3.414] Li ZH et al. You-Gui-Yin improved the reproductive dysfunction of male rats with chronic kidney disease via regulating the HIF1α-STAT5 pathway. J Ethnopharmacol. 2020 Jan 10;246:112240. WB ; Rat. PubMed:31526861 研究领域肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, 产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量92kDa细胞定位细胞核 细胞浆 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from middle of human HIF-1 Alpha:341-450/826 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator(ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.Function:Functions as a master transcriptional regulator of theadaptive response to hypoxia. Under hypoxic conditions activatesthe transcription of over 40 genes, including, erythropoietin,glucose transporters, glycolytic enzymes, vascular endothelialgrowth factor, and other genes whose protein products increaseoxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumorangiogenesis and pathophysiology of ischemic disease. Binds to coreDNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment oftranscriptional coactivators such as CREBPB and EP300. Activity isenhanced by interaction with both, NCOA1 or NCOA2. Interaction withredox regulatory protein APEX seems to activate CTAD andpotentiates activation by NCOA1 and CREBBP.Subunit:Interacts with COPS5 subunit of COP9 signalosome complex,leading to the regulation of its stability. Interacts with TSGA10(By similarity). Efficient DNA binding requires heterodimerizationof an alpha and a beta/ARNT subunit. Binds to the TAZ-type 1domains of CREBBP and EP300. Interacts with NCOA1, NCOA2, APEX andHSP90. Interacts with VHL which docks HFA1 to the E3 ubiquitinligase complex for subsequent destruction. Interaction, via the ODDdomain, with the beta domain of VHLL, protects HIF1A fromdestruction by competing against the destructive targetinginitiated by VHL.Subcellular Location:Cytoplasm. Nucleus.Tissue Specificity:Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.Post-translational modifications:In normoxia, is hydroxylated on Pro-402 and Pro-564 in theoxygen-dependent degradation domain (ODD) by EGLN1/PHD1 andEGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564.The hydroxylated prolines promote interaction with VHL, initiatingrapid ubiquitination and subsequent proteasomal degradation. Underhypoxia, proline hydroxylation is impaired and ubiquitination isattenuated, resulting in stabilization.In normoxia, is hydroxylated on Asn-803 by HIF1AN, thusabrogating interaction with CREBBP and EP300 and preventingtranscriptional activation.S-nitrosylation of Cys-800 may be responsible for increasedrecruitment of p300 coactivator necessary for transcriptionalactivity of HIF-1 complex.Acetylation of Lys-532 by ARD1 increases interaction with VHLand stimulates subsequent proteasomal degradation.Requires phosphorylation for DNA-binding.Similarity:Contains 1 basic helix-loop-helix (bHLH) domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 2 PAS (PER-ARNT-SIM) domains.SWISS:Q16665Gene ID:3091Database links:Entrez Gene: 3091 HumanEntrez Gene: 15251 MouseOmim: 603348 HumanSwissProt: Q16665 HumanSwissProt: Q61221 MouseUnigene: 597216 HumanUnigene: 3879 MouseUnigene: 446610 MouseImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.缺氧诱导因子1α不仅对于机体在缺氧条件下维持正常的生理功能具有特别重要的意义,并在肿瘤的生长以及神经细胞凋亡等病理过程中起重要作用. HIF1 alpha能调节许多下游基因的表达水平.哺乳动物细胞在低氧压力条件下出现HIF。HIF是一种转录因子，对细胞的缺氧起稳定作用。产品图片Sample:Lane 1: Hela (Human) Cell Lysate at 30 ugLane 2: A431 (Human) Cell Lysate at 30 ugLane 3: U251 (Human) Cell Lysate at 30 ugLane 4: HepG2 (Human) Cell Lysate at 30 ugPrimary: Anti-HIF-1 Alpha (bs-0737R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 120 kDObserved band size: 120 kDParaformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (bs-0737R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (bs-0737R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-HIF-1-Alpha Polyclonal Antibody, Unconjugated(bs-0737R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rat lung tissue(Smoking); 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-HIF-1-Alpha Polyclonal Antibody, Unconjugated(bs-0737R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingHela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, Unconjugated (bs-0737R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Blank control (blue line): Hela (blue).Primary Antibody (green line): Rabbit Anti- HIF-1 Alpha antibody (bs-0737R)Dilution: 1μg /10^6 cells;Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITCDilution: 1μg /test.ProtocolThe cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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