中文 
Amyloid Beta (25-35) Peptide β淀粉样肽25-35
基本售价：1500.00元
产品编号Y-0044英文名称Amyloid Beta (25-35) Peptide中文名称β淀粉样肽25-35别 名Amyloid beta Peptide (25-35); Beta-Amyloid (25-35); Amyloid beta-Protein Fragment 25-35; A beta 25-35; Beta Amyloid 25-35. Aβ淀粉样肽25-35; β淀粉样肽(25-35); Amyloid β (25-35); Aβ 25-35; β Amyloid (25-35); β Amyloid 25-35.理论分子量1.060kDa性 状Lyophilized powder序 列GSNKGAIIGLM纯化方法HPLC活性Yes保存条件Shipped at 4℃. Stored at -20℃ for one year. Avoid repeated freeze/thaw cycles.产品介绍Aβ是老年性痴呆发病的始动因子，并证实引起毒性主要部位在第25-35氨基酸之间(Aβ25-35) ,Aβ25-35(Aβ25-35 peptide)具有神经毒性，可使海马和隔区神经元的存活率下降，继而发生神经元凋亡的形态学改变.
Levofloxacin 左氧氟沙星
基本售价：80.00元
产品编号D21062英文名称Levofloxacin中文名称左氧氟沙星别 名LVFX; (-)9-氟-2,3-二氢-3-甲基-10-(4-甲基-1-哌嗪基)-7-氧-7H-吡啶骈[1,2,3-DE]-[1,4]苯骈噁嗪-6-羧酸; 可乐必妥; 洛氟沙星; 左旋氧氟沙星; 利复星;保存条件Store at RT.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍CAS：100986-85-4分子式：C18H20FN3O4分子量：361.37MDL：MFCD07772024级别：BR纯度：≥98%（HPLC）熔点：218℃沸点：571.5℃ at 760 mmHg外观：白色至淡黄色粉末溶解性：较易溶于氯仿或丙酮，微溶于乙醇，乙醚或甲醇，几不溶于水。微溶于水或甲醇。溶于冰醋酸或二氯甲烷。敏感性：光敏感用途：为全合成抗菌素，治疗各种细菌感染。
Recombinant human IL-6 protein, C-His
基本售价：3980.00元
Recombinant human IL-6 protein, C-His产品编号bs-41177P英文名称Recombinant human IL-6 protein, C-His中文名称重组人白介素6蛋白别 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6. ;Recombinant human IL-6 protein, C-His Specific References (1) | bs-41177P has been referenced in 1 publications.[IF=4.966] 222Xiaolong Zhang. et al. Baicalein inhibits non‐small‐cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment. Cancer Sci. 2020 Oct; 111(10): 3802–3812 other ; PubMed:32691974理论分子量15kDa检测分子量21 kDa性 状Lyophilized or Liquid浓 度＞0.5 mg/ml物 种Human序 列30-212/212纯 度>95% as determined by SDS-PAGE纯化方法AC内毒素Not analyzed表达系统E.coli活性Not tested标签C-His缓 冲 液20 mM Tris-HCl (pH=8.0) with 150 mM NaCl保存条件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacterium Streptococcus pneumoniae.SWISS:P05231Gene ID:3569产品图片
Recombinant mouse IL-6 protein, C-His
基本售价：1280.00元
Recombinant mouse IL-6 protein, C-His产品编号bs-10861P英文名称Recombinant mouse IL-6 protein, C-His中文名称重组小鼠白介素IL-6蛋白别 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6; Recombinant mouse IL-6 protein, C-His 理论分子量23kDa检测分子量26 kDa性 状Lyophilized or Liquid浓 度＞0.5 mg/ml物 种Mouse序 列25-211/211纯 度>95% as determined by SDS-PAGE纯化方法AC内毒素Not analyzed表达系统E.coli活性Not tested标签C-His缓 冲 液20 mM Tris-HCl with 20% Glycerol保存条件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011].SWISS:P08505Gene ID:16193产品图片
Recombinant human IL-22 protein
基本售价：4800.00元
Recombinant human IL-22 protein产品编号bs-43572P英文名称Recombinant human IL-22 protein中文名称重组人白介素22蛋白性 状Lyophilized or Liquid浓 度1mg/ml保存条件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍产品图片
Recombinant human IL-10 protein (Active, HEK293)
基本售价：2080.00元
Recombinant human IL-10 protein (Active, HEK293) 产品编号ck0006英文名称Recombinant human IL-10 protein (Active, HEK293)中文名称重组人白介素10 (活性的, Tag free)别 名Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. ;Recombinant human IL-10 protein (Active, HEK293) 人白介素10 重组蛋白; 人白细胞介素10 ; 重组人白介素10; 重组人IL-10;理论分子量17kDa检测分子量21 kDa性 状Lyophilized powder浓 度＞0.5 mg/ml物 种Human序 列19-178/178纯 度>95% as determined by SDS-PAGE纯化方法AC内毒素<0.5 EU/μg as determined by LAL.表达系统HEK293 cell活性Yes标签Tag free缓 冲 液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存条件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍Interleukin 10(IL-10) is a member of the IL-10 family of α-helical cytokines. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of a number of proinflammatory cytokines such as IFNgamma, IL-2, IL-3, TNF and GM-CSF. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.This recombinant human IL-10 is produced by human cells.(Tag free)Biological activity:The activity was measured by its ability to stimulate the proliferation of MC/9-2 cells (mouse master cell line).Reconstitution:Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.SWISS:P18893Gene ID:3586产品图片
Recombinant human IL-15 protein (Active, HEK293)
基本售价：2080.00元
Recombinant human IL-15 protein (Active, HEK293) 产品编号ck0013产品分类重组蛋白英文名称Recombinant human IL-15 protein (Active, HEK293)中文名称重组人白介素15 (活性的, Tag free)英文别名rhIL-15; Interleukin-15; IL 15; Interleukin 15; Interleukin15; MGC9721; IL15; IL15_HUMAN. ;Recombinant human IL-15 protein (Active, HEK293)中文别名人白介素15重组蛋白; 重组人白介素15; 人重组白介素15;纯化方法AC检测分子量14-15 kDaGeneID3600SwissP40933浓度＞0.5 mg/ml储存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存条件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationIsoform IL15-S48AA: Secreted. Isoform IL15-S21AA: Cytoplasm. Nucleus. Note=IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.Tissue SpecificityMost abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.SimilarityBelongs to the IL-15/IL-21 family.Database linksEntrez Gene: 3600 HumanOmim: 600554 HumanSwissProt: P40933 HumanUnigene: 654378 Human多肽活性Yes蛋白质与多肽-多肽序列49-162/162小分子偶联蛋白-蛋白载体标签Tag free纯度>95% as determined by SDS-PAGE内毒素<0.5 EU/μg as determined by LAL.重组蛋白-表达系统HEK293 cell复溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Recombinant human IL-2 protein (Active, HEK293)
基本售价：1080.00元
产品编号ck0017产品分类重组蛋白英文名称Recombinant human IL-2 protein (Active, HEK293)中文名称重组人白介素2 (活性的, Tag free)英文别名Interleukin-2; Interleukin 2; Interleukin2; rhIL-2; T-cell growth factor; T Cell Growth Factor; Aldesleukin; IL 2; IL2; Lymphokine; POIL2; T Cell Growth Factor; TCGF; Involved in regulation of T cell clonal expansion; IL2_HUMAN. ;Recombinant human IL-2 protein (Active, HEK293)中文别名人白介素2重组蛋白; 重组人白介素2; 人重组白介素2; 重组人白介素2（IL-2）;蛋白细胞定位分泌型蛋白纯化方法AC理论分子量15kDa检测分子量17 kDaGeneID3558SwissP60568浓度＞0.5 mg/ml储存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存条件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationSecreted.DISEASENote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.SimilarityBelongs to the IL-2 family.Database linksEntrez Gene: 3558 HumanEntrez Gene: 16183 MouseEntrez Gene: 116562 RatOmim: 147680 HumanSwissProt: P60568 HumanSwissProt: P04351 MouseSwissProt: P17108 RatUnigene: 89679 HumanUnigene: 14190 MouseUnigene: 9871 Rat背景资料The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]多肽活性Yes蛋白质与多肽-多肽序列21-153/153小分子偶联蛋白-蛋白载体标签Tag free纯度>95% as determined by SDS-PAGE内毒素<0.5 EU/μg as determined by LAL.重组蛋白-表达系统HEK293 cell复溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
一氧化氮合成酶-2（诱导型）抗体 | RabbitAnti-iNOS antibody
基本售价：1098.00元
产品编号bs-0162R英文名称iNOS中文名称一氧化氮合成酶-2（诱导型）抗体别 名i NOS; Nitric Oxide Synthase, Inducible; HEP NOS; Hepatocyte NOS; HEPNOS; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; INOS; Inosl; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible macrophage; Nitric oxide synthase 2A (inducible hepatocytes); Nitric oxide synthase inducible; NOS 2; NOS 2A; NOS; Nos II; NOS type II; Nos2; NOS2A; NOS2_HUMAN. Specific References (47) | bs-0162R has been referenced in 47 publications.111 [IF=2.35] Li, Guofu, et al. "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." International Journal of Molecular Medicine 38.3 (2016): 767-775. WB ; Rat. 222111 [IF=4.56] Shanshan et al. Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury. (2017) Front.Cell.Neurosc. 11:99 WB ; Rat. 222PubMed:28424593 111 [IF=2.24] Zhang et al. High altitude increases the expression of hypoxia-inducible factor 1α and inducible nitric oxide synthase with intest-inal mucosal barrier failure in rats. (2015) Int.J.Clin.Exp.Patho. 8:5189-95 IHC ; Rat. 222PubMed:26191216 111 [IF=5.64] Wang et al. CXCL1 derived from tumor-associated macrophages promotes breast cancer metastasis via activating NF-κB/SOX4 signaling. (2018) Cell.Death.Dis. 9:880 WB ; Mouse. 222PubMed:30158589 111 [IF=2.8] Liu J et al. Alterations of antioxidant indexes and inflammatory cytokine expression aggravated hepatocellular apoptosis through mitochondrial and death receptor-dependent pathways in Gallus gallus exposed to arsenic and copper. Environ Sci Pollut Res Int WB ; Chicken. 222PubMed:29569195 111 [IF=2.332] Zhao X et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.(2018) Microb Pathog.125:431-437. WB ; Mouse. 222PubMed:30316005 111 [IF=2.655] Horváth E et al. Ischemic damage and early inflammatory infiltration are different in the core and penumbra lesions of rat brain after transient focal cerebral ischemia. J Neuroimmunol. 2018 Nov 15;324:35-42. IHC-P ; Rat. 222PubMed:30212790 111 [IF=4.183] Imoto K et al. Periostin Mediates Right Ventricular Failure through Induction of Inducible Nitric Oxide SynthaseExpression in Right Ventricular Fibroblasts from Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats. Int J Mol Sci. 2018 Dec 24;20(1). IHC-P ; Rat. 222PubMed:30586863 111 [IF=3.118] Lv R et al. Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway. Int Immunopharmacol. 2019 Feb 18;70:28-36. IHC-P ; Rat. 222PubMed:30785088 111 [IF=2.535] Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523. WB ; Human. 222PubMed:31383786 111 [IF=2.914] Guo MH et al. Elemental imbalance elicited by arsenic and copper exposure lead to oxidative stress and immunotoxicity in chicken gizzard, activating the protective effects of heat shock proteins. Environmental Science and Pollution Research. 2019. WB ; Chicken. 222111 [IF=3.87] Jiao F et al. AGK2 Alleviates Lipopolysaccharide Induced Neuroinflammation through Regulation of Mitogen-Activated Protein Kinase Phosphatase-1. J Neuroimmune Pharmacol. 2019 Nov 30. IHF ; Mouse. 222PubMed:31786712 111 [IF=3.483] Huang P et al. Theoretical basis of nitrosomyoglobin formation in a dry sausage model by coagulase-negative staphylococci: Behavior and expression of nitric oxide synthase. Meat Sci. 2019 Nov 27;161:108022. WB ; coagulase-negative staphylococci (CNS) and P. pentosaceus. 222PubMed:31838366 111 [IF=3.58] El-Lakany MA et al. Ovariectomy provokes inflammatory and cardiovascular effects of endotoxemia in rats: Dissimilar benefits of hormonal supplements. Toxicol Appl Pharmacol. 2020 Feb 21;393:114928. IHC-P ; Rat. 222PubMed:32092384 111 [IF=3.361] Wang M et al. Berberine combined with cyclosporine A alleviates acute graft-versus-host disease in murine models. Int Immunopharmacol. 2020 Feb 9;81:106205. IHC-P ; Mouse. 222PubMed:32050154 111 [IF=3.761] Toru Nishikawa et al. Therapeutic potential for insulin on type 1 diabetes‐associated periodontitis: analysis of experimental periodontitis in streptozotocin‐induced diabetic rats. J Diabetes Investig . 2020 Apr 17. IHC ; rat. 222PubMed:32302049 111 [IF=8.352] Ye Hea et al. Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation. Applied Materials Today 20 (2020) 100673 IHC/IF ; Rat/Mouse. 222111 [IF=2.5] Yang F et al. The effects of binge alcohol exposure on tooth movement and associated root resorption in rats. Alcohol . 2020 Nov;88:1-9. IHC-P ; Rat. 222PubMed:32574660 111 [IF=4.225] Jiang Q et al. Particulate Matter 2.5 Induced Developmental Cardiotoxicity in Chicken Embryo and Hatchling. Front Pharmacol. 2020 Jun 5;11:841. WB ; Chicken. 222PubMed:32581800 111 [IF=] WB ; mouse. 222111 [IF=7.919] Chen Y et al. A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activityClin Transl Med.2020 Dec;10(8):e240. WB、IHC ; Mouse. 222PubMed:33377656 111 [IF=7.727] Xue Wang. et al. Engineered liposomes targeting the gut–CNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:390 WB,IHC ; Mouse. 222111 [IF=3.909] Qian Xueshen. et al. Periodontitis Deteriorates Cognitive Function and Impairs Neurons and Glia in a Mouse Model of Alzheimer’s Disease. J Alzheimers Dis. 2021 Jan;79(4):1785-1800 WB ; Mouse. 222111 [IF=3.69] Xiaoyan Xia. et al. Ameliorative effect of white tea from 50-year-old tree of Camellia sinensis L. (Theaceae) on kidney damage in diabetic mice via SIRT1/AMPK pathway. J Ethnopharmacol. 2021 May;272:113919 WB ; Mouse. 222111 [IF=8.758] Yinying Sheng. et al. Development and In Vitro Biodegradation of Biomimetic Zwitterionic Phosphorylcholine Chitosan Coating on Zn1Mg Alloy. Acs Appl Mater Inter. 2020;12(49):54445–54458 IF ; Mouse. 222111 [IF=3.368] Liu Tang. et al. Topical Application with Conjugated Linoleic Acid Ameliorates 2, 4‐dinitrofluorobenzene‐Induced Atopic Dermatitis‐like Lesions in BALB/c Mice. Exp Dermatol. 2021 Feb;30(2):237-248 WB ; Mouse. 222111 [IF=2.965] Sawada Noritaka. et al. Glucagon-Like Peptide-1 Receptor Agonist Liraglutide Ameliorates the Development of Periodontitis. J Diabetes Res. 2020;2020:8843310 IHC ; Rat. 222111 [IF=5.88] Shuai Chen. et al. Chondroitin sulfate modified 3D porous electrospun nanofiber scaffolds promote cartilage regeneration. Mat Sci Eng C-Mater. 2021 Jan;118:111312 IHC ; Rabbit. 222111 [IF=8.724] Penghui Zhang. et al. Effect of cyclic mechanical loading on immunoinflammatory microenvironment in biofabricating hydroxyapatite scaffold for bone regeneration. Bioact Mater. 2021 Oct;6:3097 WB ; Mouse. 222111 [IF=3.943] Kai Kang. et al. FGF21 alleviates chronic inflammatory injury in the aging process through modulating polarization of macrophages. Int Immunopharmacol. 2021 Jul;96:107634 WB ; Mouse. 222研究领域细胞生物 免疫学 神经生物学 信号转导 转录调节因子 激酶和磷酸酶 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, 产品应用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.理论分子量130kDa细胞定位细胞核 细胞浆 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human NOS-2: 1051-1144/1144 亚 型IgG纯化方法affinity purified by Protein A缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.PubMedPubMed 产品介绍Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).Function:Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.Subunit:Homodimer. Binds SLC9A3R1.Tissue Specificity:Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.Similarity:Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.SWISS:P35228Gene ID:4843Database links:Entrez Gene: 4843 HumanEntrez Gene: 18126 MouseEntrez Gene: 24599 RatOmim: 163730 HumanSwissProt: P35228 HumanSwissProt: P29477 MouseSwissProt: Q06518 RatUnigene: 709191 HumanUnigene: 2893 MouseUnigene: 10400 Rat合成与降解（Synthesis and Degradation）催化生物体内一氧化氮（NO）生成的酶。分神经型一氧化氮合成的酶（nNOS or NOS-1）、诱导型一氧化氮合成的酶（iNOS or NOS-2）、内皮型一氧化氮合成的酶（eNOS or NOS-3）。产品图片Sample:U251 Cell Lysate at 40 ugPrimary: Anti-iNOS(bs-0162R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 130kDObserved band size: 130kDSample:Lane 1: Mouse Raw264.7 tissue lysatesLane 2: Human Jurkat cell lysatesPrimary: Anti-iNOS (bs-0162R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 130 kDaObserved band size: 140 kDaParaformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (inos) Polyclonal Antibody, Unconjugated (bs-0162R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (iNOS) Polyclonal Antibody, Unconjugated (bs-0162R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated(bs-0162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated(bs-0162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
缺氧诱导因子1α /低氧诱导因子-1抗体 | RabbitAnti-HIF-1 Alpha antibody
基本售价：1098.00元
产品编号bs-0737R英文名称HIF-1 Alpha中文名称缺氧诱导因子1α /低氧诱导因子-1抗体别 名ARNT interacting protein; ARNT-interacting protein; Basic helix loop helix PAS protein MOP1; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF 1alpha; HIF-1-alpha; HIF1 A; HIF1 Alpha; HIF1; HIF1-alpha; HIF1A; HIF1A_HUMAN; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor); Hypoxia inducible factor1alpha; Hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor-1a; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; MOP1; PAS domain-containing protein 8; PASD 8; PASD8. Specific References (22) | bs-0737R has been referenced in 22 publications.[IF=5.595] Liu T et al. MicroRNA-493 targets STMN-1 and promotes hypoxia-induced epithelial cell cycle arrest in G/M and renal fibrosis. (2018) FASEB J. Sep 05 WB ; Mouse. PubMed:30183377 [IF=5.08] Zhang, Huimin, et al. "Vascular Normalization Induced by Sinomenine Hydrochloride Results in Suppressed Mammary Tumor Growth and Metastasis." Scientific Reports 5 (2015). IHC-P ; Mouse. PubMed:25749075 [IF=4.91] Fan, Shengjun, et al. "Opposite angiogenic outcome of curcumin against ischemia and Lewis lung cancer models:in silico, in vitro and in vivo studies." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2014). WB ; Mouse. PubMed:24970744 [IF=4.556] Yang X et al. Synthesis and bioevaluation of novel 18FDG-conjugated 2-nitroimidazole derivatives for tumor-hypoxia imaging. Mol Pharm. 2019 May 6;16(5):2118-2128. IHC&IF ; Mouse&Human. PubMed:30964298 [IF=4.539] Talwar H et al. MEK2 Negatively Regulates Lipopolysaccharide-Mediated IL-1β Production through HIF-1α Expression. J Immunol. 2019 Mar 15;202(6):1815-1825. WB&IP ; Mouse. PubMed:30710049 [IF=4.44] Madka, Venkateshwar, et al. "Targeting mTOR and p53 signaling inhibits muscle invasive bladder cancer in vivo." Cancer Prevention Research 9.1 (2016): 53-62. IHC-P ; Mouse. PubMed:26577454 [IF=4.19] Talwar, Harvinder, et al. "MKP-1 negatively regulates LPS-mediated IL-1β production through p38 activation and HIF-1α expression." Cellular Signalling (2017). Mouse. PubMed:28238855 [IF=3.743] Wang D et al. Effects of hypoxia and ASIC3 on nucleus pulposus cells: From cell behavior to molecularmechanism. Biomed Pharmacother. 2019 Jun 12;117:109061. WB ; Rabbit. PubMed:31202172 [IF=3.53] Woolf, Eric C., et al. "The Ketogenic Diet Alters the Hypoxic Response and Affects Expression of Proteins Associated with Angiogenesis, Invasive Potential and Vascular Permeability in a Mouse Glioma Model." PLOS ONE10.6 (2015): e0130357. WB ; Mouse. PubMed:26083629 [IF=3.288] Chai D et al. β2-microglobulin has a different regulatory molecular mechanism between ER+ and ER- breast cancer with HER2.BMC Cancer. 2019 Mar 12;19(1):223. IHC-P ; Human. PubMed:30866857 [IF=2.989] Ju X et al. Catalpol Promotes the Survival and VEGF Secretion of Bone Marrow-Derived Stem Cells and Their Role in Myocardial Repair After Myocardial Infarction in Rats.Cardiovasc Toxicol. 2018 May 11. WB ; Rat. PubMed:29752623 [IF=2.91] Shou, Zhu, et al. "Expression and prognosis of FOXO3a and HIF-1?? in nasopharyngeal carcinoma."Journal of cancer research and clinical oncology 138.4 (2012): 585-593.. WB ; Human. PubMed:22209974 [IF=2.784] Yang D et al. Normobaric oxygen inhibits AQP4 and NHE1 expression in experimental focal ischemic stroke. (2018) Int. J. Mol. Med. WB ; Rat . PubMed:30592266 [IF=2.766] Dao DT et al. A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor.(2018) PLoS One. WB ; Mouse. PubMed:30566445 [IF=2.566] Yang Z et al. Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1ɑ axis in esophageal squamous cell carcinoma.Exp Mol Pathol. 2019 Mar 20. WB ; Human. PubMed:30904401 [IF=2.49] Yang, Ya, et al. "Expression of RAP1B is associated with poor prognosis and promotes an aggressive phenotype in gastric cancer." Oncology reports 34.5 (2015): 2385-2394. IHC-P ; Human. PubMed:26329876 [IF=1.64] Guo, Wei, et al. "Transplantation of endothelial progenitor cells in treating rats with IgA nephropathy." BMC Nephrology 15.1 (2014): 110. WB ; Rat. PubMed:25012471 [IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082. WB ; Rat. PubMed:27665780 [IF=1.41] Song et al. Effects of HSYA on the proliferation and apoptosis of MSCs exposed to hypoxic and serum deprivation conditions. (2018) Exp.Ther.Med. 15:5251-5260 WB ; Rat. PubMed:29904409 [IF=.5] Talwar, Harvinder, et al. "The dataset describes: HIF-1 α expression and LPS mediated cytokine production in MKP-1 deficient bone marrow derived murine macrophages." Data in Brief (2017). WB ; Mouse. PubMed:28765831 [IF=0] Talwar et al. The dataset describes: HIF-1 α expression and LPS mediated cytokine production in MKP-1 deficient bone marrow derived murine macrophages. (2017) Data.Brie. 14:56-61 WB ; Mouse. PubMed:28765831 [IF=3.414] Li ZH et al. You-Gui-Yin improved the reproductive dysfunction of male rats with chronic kidney disease via regulating the HIF1α-STAT5 pathway. J Ethnopharmacol. 2020 Jan 10;246:112240. WB ; Rat. PubMed:31526861 研究领域肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, 产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量92kDa细胞定位细胞核 细胞浆 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from middle of human HIF-1 Alpha:341-450/826 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator(ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.Function:Functions as a master transcriptional regulator of theadaptive response to hypoxia. Under hypoxic conditions activatesthe transcription of over 40 genes, including, erythropoietin,glucose transporters, glycolytic enzymes, vascular endothelialgrowth factor, and other genes whose protein products increaseoxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumorangiogenesis and pathophysiology of ischemic disease. Binds to coreDNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment oftranscriptional coactivators such as CREBPB and EP300. Activity isenhanced by interaction with both, NCOA1 or NCOA2. Interaction withredox regulatory protein APEX seems to activate CTAD andpotentiates activation by NCOA1 and CREBBP.Subunit:Interacts with COPS5 subunit of COP9 signalosome complex,leading to the regulation of its stability. Interacts with TSGA10(By similarity). Efficient DNA binding requires heterodimerizationof an alpha and a beta/ARNT subunit. Binds to the TAZ-type 1domains of CREBBP and EP300. Interacts with NCOA1, NCOA2, APEX andHSP90. Interacts with VHL which docks HFA1 to the E3 ubiquitinligase complex for subsequent destruction. Interaction, via the ODDdomain, with the beta domain of VHLL, protects HIF1A fromdestruction by competing against the destructive targetinginitiated by VHL.Subcellular Location:Cytoplasm. Nucleus.Tissue Specificity:Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.Post-translational modifications:In normoxia, is hydroxylated on Pro-402 and Pro-564 in theoxygen-dependent degradation domain (ODD) by EGLN1/PHD1 andEGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564.The hydroxylated prolines promote interaction with VHL, initiatingrapid ubiquitination and subsequent proteasomal degradation. Underhypoxia, proline hydroxylation is impaired and ubiquitination isattenuated, resulting in stabilization.In normoxia, is hydroxylated on Asn-803 by HIF1AN, thusabrogating interaction with CREBBP and EP300 and preventingtranscriptional activation.S-nitrosylation of Cys-800 may be responsible for increasedrecruitment of p300 coactivator necessary for transcriptionalactivity of HIF-1 complex.Acetylation of Lys-532 by ARD1 increases interaction with VHLand stimulates subsequent proteasomal degradation.Requires phosphorylation for DNA-binding.Similarity:Contains 1 basic helix-loop-helix (bHLH) domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 2 PAS (PER-ARNT-SIM) domains.SWISS:Q16665Gene ID:3091Database links:Entrez Gene: 3091 HumanEntrez Gene: 15251 MouseOmim: 603348 HumanSwissProt: Q16665 HumanSwissProt: Q61221 MouseUnigene: 597216 HumanUnigene: 3879 MouseUnigene: 446610 MouseImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.缺氧诱导因子1α不仅对于机体在缺氧条件下维持正常的生理功能具有特别重要的意义,并在肿瘤的生长以及神经细胞凋亡等病理过程中起重要作用. HIF1 alpha能调节许多下游基因的表达水平.哺乳动物细胞在低氧压力条件下出现HIF。HIF是一种转录因子，对细胞的缺氧起稳定作用。产品图片Sample:Lane 1: Hela (Human) Cell Lysate at 30 ugLane 2: A431 (Human) Cell Lysate at 30 ugLane 3: U251 (Human) Cell Lysate at 30 ugLane 4: HepG2 (Human) Cell Lysate at 30 ugPrimary: Anti-HIF-1 Alpha (bs-0737R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 120 kDObserved band size: 120 kDParaformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (bs-0737R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIF-1 Alpha) Polyclonal Antibody, Unconjugated (bs-0737R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-HIF-1-Alpha Polyclonal Antibody, Unconjugated(bs-0737R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rat lung tissue(Smoking); 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-HIF-1-Alpha Polyclonal Antibody, Unconjugated(bs-0737R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingHela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, Unconjugated (bs-0737R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Blank control (blue line): Hela (blue).Primary Antibody (green line): Rabbit Anti- HIF-1 Alpha antibody (bs-0737R)Dilution: 1μg /10^6 cells;Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITCDilution: 1μg /test.ProtocolThe cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
I型胶原蛋白抗体 | RabbitAnti-Collagen I antibody
基本售价：1098.00元
产品编号bs-0578R英文名称Collagen I中文名称I型胶原蛋白抗体别 名Collagen type I; Alpha 1 type I collagen; Alpha 2 type I collagen; COL1A1; COL1A2; Collagen I alpha 1 polypeptide; Collagen I alpha 2 polypeptide; Collagen Of Skin Tendon And Bone; Collagen Type 1; Collagen type I alpha 1; Collagen type I alpha 2; OI4; Osteogenesis Imperfecta Type IV; Pro alpha 1(I) collagen; Type I procollagen; CO1A1_HUMAN; Collagen alpha-1(II) chain; Alpha-1 type II collagen; Collagen alpha-1(II) chain; Chondrocalcin; collagen alpha-1(I) chain preproprotein; Collagen Ⅰ. 胶原蛋白1; 1型胶原蛋白; I型胶原;Specific References (118) | bs-0578R has been referenced in 118 publications.111 [IF=7.7] Yang, Fan, et al. "Strontium enhances osteogenic differentiation of mesenchymal stem cells and in vivo bone formation by activating Wnt/catenin signaling." Stem Cells 29.6 (2011): 981-991. Human, Rat. 222111 [IF=3.82] Luo, Yang, et al. "Alendronate Retards the Progression of Lumbar Intervertebral Disc Degeneration in Ovariectomized Rats." Bone (2013). IHC-P ; Rat. 222111 [IF=3.61] Xu, Zhen E., et al. "Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice." American Journal of Physiology-Renal Physiology 301.4 (2011): F713-F722. IHC-P ; Mouse. 222111 [IF=3.55] Guo, Hong-Feng, et al. "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." Colloids and Surfaces B: Biointerfaces (2012). WB ; Mouse. 222111 [IF=1.97] Zhang, Qiang-Ye, et al. "Administration of a novel penicillamine-bound membrane: a preventive and therapeutic treatment for abdominal adhesions." BMC surgery 11.1 (2011): 5. Rat. 222111 [IF=2.16] Zhou, Nan, et al. "Involvement of Imperatorin Derivative‐OW1 in the antihypertensive effect and vascular remodeling of renovascular hypertension rats." Clinical and Experimental Pharmacology and Physiology (2014). IHC-P ; Rat. 222111 [IF=4.45] Wang, Chen-Chie, et al. "Expandable Scaffold Improves Integration of Tissue Engineered Cartilage: An In Vivo Study in A Rabbit Model." Tissue Engineering ja (2016). IHC-P ; Rabbit. 222111 [IF=2.07] Chen, Cheng, et al. "Effects of Cytoskeleton Modulation on the Phenotype of Articular Chondrocytes in Monolayer and Pellet Culture." Journal of Biomaterials and Tissue Engineering 5.12 (2015): 942-950. IHC-P ; Rabbit. 222111 [IF=1.93] Gao, Yuanyuan, et al. "TGF‐β1 promotes bovine mammary fibroblast proliferation through the ERK 1/2 signalling pathway." Cell biology international (2016). IHC-P ; Bovine. 222111 [IF=.68] Çakir-Özkan, Nilüfer, et al. "Therapeutic Effects of Sildenafil on Experimental Mandibular Fractures." Journal of Craniofacial Surgery 27.3 (2016): 615-620. IHC-P ; Rat. 222111 [IF=3.42] Cecen, Berivan, et al. "Biocompatibility and biomechanical characteristics of loofah based scaffolds combined with hydroxyapatite, cellulose, poly-l-lactic acid with chondrocyte-like cells." Materials Science and Engineering: C(2016). IHC ; Human. 222111 [IF=1.68] Ren, Xiang, et al. "Engineering zonal cartilage through bioprinting collagen type II hydrogel constructs with biomimetic chondrocyte density gradient."BMC Musculoskeletal Disorders 17.1 (2016): 1. IHC-P ; Rabbit. 222111 [IF=1.28] Tian, Fa‑Ming, et al. "Orally administered simvastatin partially preserves lumbar vertebral bone mass but not integrity of intervertebral discs in ovariectomized rats." Experimental and Therapeutic Medicine. IHC-P ; Rat. 222111 [IF=3.365] Yang, Ning, et al. "Caffeic acid phenethyl ester up-regulates antioxidant levels in hepatic stellate cell line T6 via an Nrf2-mediated mitogen activated protein kinases pathway." World J Gastroenterol 23.7 (2017): 1203-1214. WB ; Rat. 222111 [IF=5.168] Yu, Chengyuan, et al. "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." Oncotarget 8.23 (2017): 37705. WB ; Dog. 222111 [IF=2.134] Wang et al. The influence of hypoxia during different pregnancy stages on cardiac collagen accumulation in the adult offspring. (2014) Biomed.Res.In. 2014:419805 WB ; Rat. 222PubMed:25013780111 [IF=.971] Yang et al. Isolation and biological characterization of tendon-derived stem cells from fetal bovine. (2016) In.Vitro.Cell.Dev.Biol.Ani. 52:846-56 IF(ICC) ; Rat. 222PubMed:27130678111 [IF=1.922] Chi et al. Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. (2018) Mol.Med.Rep. 17:6337-6344 IHC ; Human. 222PubMed:29512784111 [IF=3.952] Yang KC et al. Low-adhesive ethylene vinyl alcohol-based packaging to xenogeneic islet encapsulation for type 1 diabetes treatment.Biotechnol Bioeng. 2018 Sep;115(9):2341-2355. IHC-P ; Rat. 222PubMed:29777589111 [IF=1.792] Huo YT et al. Methacholine induces extracellular matrix production by human airway smooth muscle cells through -catenin signaling.Respiratory Physiology & Neurobiology, 254, 55–63. IF ; Human. 222111 [IF=3.234] Fan S et al. Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway.Life Sci. 2018 Aug 15;207:205-211. WB ; Rat. 222PubMed:29883721111 [IF=2.25] Zhang X et al. Quercetin ameliorates pulmonary fibrosis by inhibiting SphK1/S1P signaling.Biochem Cell Biol. 2018 Dec;96(6):742-751. WB ; Mouse&Human. 222PubMed:29940125111 [IF=2.634] Ma C et al. Identification and Multilineage Potential Research of a Novel Type of Adipose-Derived Mesenchymal Stem Cells from Goose Inguinal Groove. DNA Cell Biol. 2018 Sep;37(9):731-741. ICF ; Goose. 222PubMed:30102556111 [IF=1.664] Gao S et al. Abnormal changes in the quantity and function of osteoblasts cultured in patients with myelodysplastic syndrome. Oncol Lett. 2018 Oct;16(4):4384-4390. ICC ; Human. 222PubMed:30214573111 [IF=3.166] Wang Y et al. ASIC1a promotes high glucose and PDGF-induced hepatic stellate cell activation by inducing autophagy through CaMKKβ/ERK signaling pathway. (2018) Toxicol Lett. (18)31963-5 WB ; Rat. 222PubMed:30291941111 [IF=4.427] Wu M et al. Synergistic effects of sulfur dioxide and polycyclic aromatic hydrocarbons on pulmonary pro-fibrosis via mir-30c-1-3p/transforming growth factor β type II receptor axis.(2018) Chemosphere 219 WB ; Mouse&Human. 222PubMed:30543962111 [IF=3.373] Niu L et al. PLGA/PLCA casting and PLGA/PDPA electrospinning bilayer film for prevention of postoperative adhesion. (2018) J. Biomed. Mater. Res. Part B Appl. Biomater. IHC-P ; Rabbit. 222PubMed:30548816111 [IF=1.564] Liu P et al. Matrine Suppresses Pancreatic Fibrosis by Regulating TGF-β/Smad Signaling in Rats. (2019) Yonsei Med. J. 60(1) IHC-P ; Rat. 222PubMed:30554494111 [IF=3.909] Kara A et al. Bioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineering. Int J Biol Macromol. 2019 Feb 20;130:266-279. SEM, fluorescence microscopy&IHC ; Fish(Sparus aurata). 222PubMed:30797008111 [IF=4.938] Li Y et al. Klotho recovery by genistein via promoter histone acetylation and DNA demethylation mitigates renal fibrosis in mice.J Mol Med (Berl). 2019 Apr;97(4):541-552. WB ; Mouse&Human. 222PubMed:30806715研究领域细胞生物 免疫学 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Dog, Rabbit, (predicted: Cow, Horse, Sheep, Monkey, )产品应用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.理论分子量130kDa细胞定位细胞外基质 分泌型蛋白 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human Collagen I C-terminal propeptide: 1321-1400/1464 亚 型IgG纯化方法affinity purified by Protein A缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.PubMedPubMed产品介绍This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008].Function:Type I collagen is a member of group I collagen (fibrillar forming collagen).Subunit:Trimers of one alpha 2(I) and two alpha 1(I) chains. Interacts with MRC2. Interacts with TRAM2.Subcellular Location:Secreted, extracellular space, extracellular matrix.Tissue Specificity:Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.Post-translational modifications:Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.DISEASE:Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.Defects in COL1A1 are a cause of osteogenesis imperfecta type 2 (OI2) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.Similarity:Belongs to the fibrillar collagen family.Contains 1 fibrillar collagen NC1 domain.Contains 1 VWFC domain.SWISS:P02452Gene ID:1277Database links:· Entrez Gene: 1277 Human· Entrez Gene: 12842 Mouse· Entrez Gene: 100008952 Rabbit· Entrez Gene: 29393 Rat· Omim: 120150 Human· SwissProt: P02453 Cow· SwissProt: O46392 Dog· SwissProt: P02452 Human· SwissProt: P11087 Mouse· SwissProt: P02454 Rat· Unigene: 172928 Human· Unigene: 277735 Mouse· Unigene: 107239 RatI型胶原是软骨基质中的一种结构蛋白 。主要用于Ⅰ型胶原分布及变态反应方面的研究。产品图片Sample:Lane 1: HepG2 (Human) Cell Lysate at 30 ugPrimary: Anti-Collagen I (bs-0578R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 130,110 kDObserved band size: 130 kDSample:Lane 1: Placenta (Mouse) Lysate at 40 ugLane 2: U-2OS (Human) Cell Lysate at 30 ugPrimary: Anti-Collagen I (bs-0578R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 130,110 kDObserved band size: 130 kDTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Collagen I Polyclonal Antibody, Unconjugated(bs-0578R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Collagen I Polyclonal Antibody, Unconjugated(bs-0578R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Ⅱ型胶原蛋白抗体 | RabbitAnti-Collagen II antibody
基本售价：1098.00元
产品编号bs-10589R英文名称Collagen II中文名称Ⅱ型胶原蛋白抗体别 名Collagen II alpha 1; COL2A1; COL2A1 protein; collagen, type II, alpha 1; collagen alpha-1(II); type II collagen; alpha-1 type II collagen; alpha1 type II collagen; Col2a1; AOM; Cartilage collagen; Chondrocalcin; COL11A3; Collagen alpha 1(II) chain precursor; Collagen II alpha 1 polypeptide; Collagen type II alpha 1 (primary osteoarthritis spondyloepiphyseal dysplasia congenital); MGC131516; SEDC; CO2A1_HUMAN. Specific References (7) | bs-10589R has been referenced in 7 publications.[IF=4.263] Wu X et al.The role of Ca 2+ in acid-sensing ion channel 1a-mediated chondrocyte pyroptosis in rat adjuvant arthritis.(2018) Lab Invest. WB ; Rat . PubMed:30487596 [IF=3.76] Shi, Yang, et al. "Hypoxia combined with spheroid culture improves cartilage specific function in chondrocytes." Integrative Biology 7.3 (2015): 289-297. Rat. PubMed:25614382 [IF=2.784] Ma C et al.Isolation and biological characteristic evaluation of a novel type of cartilage stem/progenitor cell derived from Small‑tailed Han sheep embryos.Int J Mol Med. 2018 Jul;42(1):525-533. ICF ; Sheep. PubMed:29693133 [IF=2.348] Ren et al. Treatment of rabbit intervertebral disc degeneration with co-transfection by adeno-associated virus-mediated SOX9 and osteogenic protein-1 double genes in vivo. (2013) Int.J.Mol.Me. 32:1063-8 WB ; Rabbit. PubMed:24045878 [IF=0] Xu et al. Treatment with SiMiaoFang, an anti-arthritis chinese herbal formula, inhibits cartilage matrix degradation in osteoarthritis rat model. (2013) Rejuvenation.Re. 16:364-76 IHC-P ; Rat. PubMed:23799821 [IF=0] Yang et al. Isolation and biological characterization of tendon-derived stem cells from fetal bovine. (2016) In.Vitro.Cell.Dev.Biol.Ani. 52:846-56 IF(ICC) ; Rat. PubMed:27130678 [IF=] Luo P et al. IL-37b alleviates inflammation in the temporomandibular joint cartilage via IL-1R8 pathway. Cell Prolif. 2019 Sep 27:e12692. IHC-P ; Rat. PubMed:31560411 研究领域肿瘤 细胞生物 免疫学 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, 产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量117kDa细胞定位细胞外基质 分泌型蛋白 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human Collagen II:231-330/1487 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.Function:Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.Subunit:Homotrimers of alpha 1(II) chains.Subcellular Location:Secreted, extracellular space, extracellular matrix.Tissue Specificity:Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.Post-translational modifications:Probably 3-hydroxylated on prolines by LEPREL1 (By similarity). Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2.DISEASE:Defects in COL2A1 are the cause of spondyloepiphyseal dysplasia congenital type (SEDC) [MIM:183900]. This disorder is characterized by disproportionate short stature and pleiotropic involvement of the skeletal and ocular systems.Defects in COL2A1 are the cause of spondyloepimetaphyseal dysplasia Strudwick type (SEMD-STR) [MIM:184250]. A bone disease characterized by disproportionate short stature from birth, with a very short trunk and shortened limbs, and skeletal abnormalities including lordosis, scoliosis, flattened vertebrae, pectus carinatum, coxa vara, clubfoot, and abnormal epiphyses or metaphyses. A distinctive radiographic feature is irregular sclerotic changes, described as dappled in the metaphyses of the long bones.Defects in COL2A1 are the cause of achondrogenesis type 2 (ACG2) [MIM:200610]; also known as achondrogenesis-hypochondrogenesis type II. ACG2 is a disease characterized by the absence of ossification in the vertebral column, sacrum and pubic bones.Defects in COL2A1 are the cause of Legg-Calve-Perthes disease (LCPD) [MIM:150600]; also known as Legg-Perthes disease or Perthes disease. LCPD is characterized by loss of circulation to the femoral head, resulting in avascular necrosis in a growing child. Clinical pictures of the disease vary, depending on the phase of disease progression through ischemia, revascularization, fracture and collapse, and repair and remodeling of the bone.Defects in COL2A1 are the cause of Kniest dysplasia (KD) [MIM:156550]; also known as Kniest syndrome or metatropic dwarfism type II. KD is a moderately severe chondrodysplasia phenotype that results from mutations in the COL2A1 gene. Characteristics of the disorder include a short trunk and extremities, mid-face hypoplasia, cleft palate, myopia, retinal detachment, and hearing loss.Defects in COL2A1 are a cause of primary avascular necrosis of femoral head (ANFH) [MIM:608805]; also known as ischemic necrosis of the femoral head or osteonecrosis of the femoral head. ANFH causes disability that often requires surgical intervention. Most cases are sporadic, but families in which there is an autosomal dominant inheritance of the disease have been identified. It has been estimated that 300,000 to 600,000 people in the United States have ANFH. Approximately 15,000 new cases of this common and disabling disorder are reported annually. The age at the onset is earlier than that for osteoarthritis. The diagnosis is typically made when patients are between the ages of 30 and 60 years. The clinical manifestations, such as pain on exertion, a limping gait, and a discrepancy in leg length, cause considerable disability. Moreover, nearly 10 percent of the 500,000 total-hip arthroplasties performed each year in the United States involve patients with ANFH. As a result, this disease creates a substantial socioeconomic cost as well as a burden for patients and their families.Defects in COL2A1 are the cause of osteoarthritis with mild chondrodysplasia (OACD) [MIM:604864]. Osteoarthritis is a common disease that produces joint pain and stiffness together with radiologic evidence of progressive degeneration of joint cartilage. Some forms of osteoarthritis are secondary to events such as trauma, infections, metabolic disorders, or congenital or heritable conditions that deform the epiphyses or related structures. In most patients, however, there is no readily identifiable cause of osteoarthritis. Inheritance in a Mendelian dominant manner has been demonstrated in some families with primary generalized osteoarthritis. Reports demonstrate coinheritance of primary generalized osteoarthritis with specific alleles of the gene COL2A1, the precursor of the major protein of cartilage.Defects in COL2A1 are the cause of platyspondylic lethal skeletal dysplasia Torrance type (PLSD-T) [MIM:151210]. Platyspondylic lethal skeletal dysplasias (PLSDs) are a heterogeneous group of chondrodysplasias characterized by severe platyspondyly and limb shortening. PLSD-T is characterized by varying platyspondyly, short ribs with anterior cupping, hypoplasia of the lower ilia with broad ischial and pubic bones, and shortening of the tubular bones with splayed and cupped metaphyses. Histology of the growth plate typically shows focal hypercellularity with slightly enlarged chondrocytes in the resting cartilage and relatively well-preserved columnar formation and ossification at the chondro-osseous junction. PLSD-T is generally a perinatally lethal disease, but a few long-term survivors have been reported.Defects in COL2A1 are the cause of multiple epiphyseal dysplasia with myopia and conductive deafness (EDMMD) [MIM:132450]. Multiple epiphyseal dysplasia is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDMMD is an autosomal dominant disorder characterized by epiphyseal dysplasia associated with progressive myopia, retinal thinning, crenated cataracts, conductive deafness.Defects in COL2A1 are the cause of spondyloperipheral dysplasia (SPD) [MIM:271700]. SPD patients manifest short stature, midface hypoplasia, sensorineural hearing loss, spondyloepiphyseal dysplasia, platyspondyly and brachydactyly.Similarity:Belongs to the fibrillar collagen family.Contains 1 fibrillar collagen NC1 domain.Contains 1 VWFC domain.SWISS:P02458Gene ID:1280Database links:Entrez Gene: 1280 HumanEntrez Gene: 12824 MouseEntrez Gene: 25412 RatOmim: 120140 HumanSwissProt: P02458 HumanSwissProt: P28481 MouseSwissProt: P05539 RatUnigene: 408182 HumanUnigene: 2423 MouseUnigene: 10124 RatImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品图片Sample:293T(Human) Cell Lysate at 30 ugA549 (Human) Cell Lysate at 30 ugLOVO (Human) Cell Lysate at 40 ugMCF-7 (Human) Cell Lysate at 30 ugHepG2 (Human) Cell Lysate at 30 ugPrimary: Anti-Collagen II (bs-10589R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 117 kDObserved band size: 117 kDSample:MG63 (Human) Cell Lysate at 30 ugPrimary: Anti-Collagen II (bs-10589R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 117 kDObserved band size: 117 kDParaformaldehyde-fixed, paraffin embedded (mouse ear); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Collagen II) Polyclonal Antibody, Unconjugated (bs-10589R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: Mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;Incubation: Anti-Collagen II Polyclonal Antibody, Unconjugated(bs-10589R) 1:100, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Bax抗体 Bax | RabbitAnti-Bax antibody
基本售价：1098.00元
产品编号bs-0127R英文名称Bax中文名称Bax抗体别 名apoptosis regulator BAX; Apoptosis regulator BAX cytoplasmic isoform beta; Apoptosis regulator BAX membrane isoform alpha; Bax isoform psi; BAX protein cytoplasmic isoform delta; Bax protein cytoplasmic isoform delta. antibody Bax protein cytoplasmic isoform gamma; Bax zeta; Bax-protein; Bcl-2-like protein 4; BCL2 associated X protein; BCL2L4; BAX_HUMAN; Bcl2-L-4. Specific References (68) | bs-0127R has been referenced in 68 publications.[IF=7.519] Goreczny,et al.Hic-5 regulates fibrillar adhesion formation to control tumor extracellular matrix remodeling through interaction with tensin1.(2018) Oncogene. 37:1699-1713. IF(ICC) ; Human. PubMed:29348458 [IF=6.375] Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203. IHC-P + WB ; Mouse. PubMed:29317253 [IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93. WB ; Mouse. PubMed:30791957 [IF=5.62] Qiu, Shi, et al. "A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53." Cancer Letters 356.2 (2015): 910-917. IP ; Human. PubMed:25444901 [IF=5.29] Huang, Chuen-Lin, et al. "Paraquat Induces Cell Death Through Impairing Mitochondrial Membrane Permeability." Molecular Neurobiology (2015): 1-20. Rat. PubMed:25947082 [IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458 WB ; Human. PubMed:26881030 [IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458 WB ; Human. PubMed:26881030 [IF=4.47] Park, Min-Jung, et al. "New role of irisin in hepatocytes: The protective effect of hepatic steatosis in vitro." Cellular Signalling (2015). WB ; Mouse. PubMed:25917316 [IF=4.12] Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543 WB ; Human. PubMed:27824118 [IF=3.909] Zhu B et al. The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9. IHF ; Rat. PubMed:30851331 [IF=3.83] Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653 WB ; Human. PubMed:28974930 [IF=3.73] Rani, Neha, et al. "Regulation of Heat Shock Proteins 27 and 70, p-Akt/p-eNOS and MAPKs by Naringin Dampens Myocardial Injury and Dysfunction In Vivo after Ischemia/Reperfusion." PLOS ONE 8.12 (2013): e82577. WB ; Rat. PubMed:24324809 [IF=3.73] Zhu, Zhongling, et al. "Regulation of Cell Transformation by Rb-Controlled Redox Homeostasis." PLOS ONE 9.7 (2014): e102582. WB ; Mouse. PubMed:25019272 [IF=3.7] Lin, Lai-xiang, et al. "Feasibility of β-Sheet Breaker Peptide-H102 Treatment for Alzheimers Disease Based on β-Amyloid Hypothesis." PLoS one 9.11 (2014): e112052. IHC-P ; Mouse. PubMed:25372040 [IF=3.56] He, Xin, et al. "Canonical Wnt signaling pathway contributes to the proliferation and survival in porcine pancreatic stem cells (PSCs)." Cell and tissue research 362.2 (2015): 379-388. WB ; Pig. PubMed:26085341 [IF=3.457] Chai FN et al. Coptisine from Rhizoma coptidis exerts an anti-cancer effect on hepatocellular carcinoma by up-regulating miR-122.Biomed Pharmacother. 2018 Jul;103:1002-1011. WB ; Mouse. PubMed:29710498 [IF=3.412] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. ameliorated D-galactose-induced memory impairment, oxidative stress, and neuroinflammation in adult rats.J Agric Food Chem. 2019 Mar 20;67(11):3140-3149. WB&IHC-P ; Rat. PubMed:30813721 [IF=3.33] Chen, Jie, et al. "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." BMC cancer 12.1 (2012): 464. WB ; Human. PubMed:23050783 [IF=3.33] Tian, Xiao Qiang, et al. "The protective effect of Hyperbaric oxygen and Ginkgo Biloba extract on Aβ25-35-induced oxidative stress and neuronal apoptosis in rats." Behavioural Brain Research (2012). WB ; Rat. PubMed:23266522 [IF=3.265] Zeng Q et al.Long-term arsenite exposure induces testicular toxicity by redox imbalance, G2/M cell arrest and apoptosis in mice.(2018) Toxicology. (18)30396-2. WB ; Mouse. PubMed:30278210 [IF=3.265] Si J et al. Effects of ionizing radiation and HLY78 on the zebrafish embryonic developmental toxicity.(2018) Toxicology. 2018 Oct 12. WB ; zebrafish . PubMed:30321647 [IF=3.23] Daverey, Amita, and Sandeep K. Agrawal. "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." Neuroscience 333 (2016): 92-103. WB ; Human. PubMed:27423629 [IF=3.208] Cui H et al. Gamma linolenic acid regulates PHD2 mediated hypoxia and mitochondrial apoptosis in DENinduced hepatocellular carcinoma. Drug Des Devel Ther. 2018 Dec 13;12:4241-4252. WB ; Rat. PubMed:30587920 [IF=3.156] Andharia,et al.Electrophysiological properties of anion exchangers in the luminal membrane of guinea pig pancreatic duct cells.(2018) Pflugers Archiv: European Journal of Physiology. 470:897-907. IHC-P + WB ; Guinea Pig. PubMed:29399744 [IF=3.138] Chen J et al. Effect of artemisinin on proliferation and apoptosis-related protein expression in vivo and in vitro.Saudi J Biol Sci. 2018 Nov;25(7):1488-1493. WB&IHC ; Rat. PubMed:30505200 [IF=3.13] Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017). WB ; Human. PubMed:28887916 [IF=3.105] Yang F et al. Copper induces oxidative stress and apoptosis through mitochondria-mediated pathway in chicken hepatocytes.(2019)Toxicol In Vitro. Feb;54:310-316. WB ; Chicken. PubMed:30389602 [IF=3.06] Li, Guiying, et al. "Gremlin Aggravates Hyperglycemia‐Induced Podocyte Injury by a TGFβ/Smad Dependent Signaling Pathway." Journal of Cellular Biochemistry (2013). WB ; Mouse. PubMed:23553804 [IF=2.86] Liu, Yaping, et al. "Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice." International Journal of Molecular Sciences 17.1 (2016): 96. WB ; Mouse. PubMed:26771609 [IF=2.85] Li et al. A plasma mir-125a-5p as a novel biomarker for Kawasaki disease and induces apoptosis in HUVECs. (2017) PLoS.On. 12:e0175407 WB ; Human. PubMed:28467514 [IF=2.76] Liu, Qiang, et al. "Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis." Biomedicine & Pharmacotherapy(2017). WB ; Rat. PubMed:29198746 [IF=2.75] Ye, Bengui, et al. "Anti-tumor activity and relative mechanism of ethanolic extract of Marsdenia tenacissima (Asclepiadaceae) against human hematologic neoplasm in vitro and in vivo." Journal of Ethnopharmacology (2014). WB ; Human. PubMed:24583069 [IF=2.69] Lin, Caiyu, et al. "Lithocarpus Polystachyus Rehd Leaf Aqueous Extract Inhibits Human Breast Cancer Growth In Vitro and In Vivo." Nutrition and Cancer (2014). WB ; Human. PubMed:24660968 [IF=2.656] Liu et al. Effects of insulin-like growth factor binding protein 3 on apoptosis of cutaneous squamous cell carcinoma cells. (2018) Onco.Targets.Ther. 11:6569-6577 WB ; PubMed:30323629 [IF=2.62] Rahman, Md Mostafizur, et al. "Cytotoxic effects of cadmium and zinc co-exposure in PC12 cells and the underlying mechanism." Chemico-Biological Interactions (2017). WB ; Rat. PubMed:28390674 [IF=2.559] Jia C et al. Enhanced antitumor effect of combination of annexin A1 knockdown and bortezomib treatment in multiple myeloma in vitro and in vivo(2018) Biochem Biophys Res Commun.505(3):720-725 WB ; Mouse. PubMed:30292410 [IF=2.491] Şahin S et al. Vitamin D protects against hippocampal apoptosis related with seizures induced by kainic acid and pentylenetetrazol in rats.(2018) Epilepsy Res.149 IHC-P ; Rat . PubMed:30584976 [IF=2.38] She, Yue, et al. "Aluminum Trichloride Disorders Bile Acid Secretion and Induces Hepatocyte Apoptosis in Rats." Cell Biochemistry and Biophysics (2015): 1-9. IHC-P ; Rat. PubMed:25578489 [IF=2.38] Abdel-Hamid, Nagwa I., Mona F. El-Azab, and Yasser M. Moustafa. "Macrolide antibiotics differentially influence human HepG2 cytotoxicity and modulate intrinsic/extrinsic apoptotic pathways in rat hepatocellular carcinoma model." Naunyn-Schmiedeberg's Archives of Pharmacology (2017): 1-17. IHC-P ; Rat. PubMed:28070612 [IF=2.27] He, X., et al. "Rapamycin inhibits acrolein‐induced apoptosis by alleviating ROS‐driven mitochondrial dysfunction in male germ cells." Cell Proliferation (2014). WB ; Mouse. PubMed:24483236 [IF=2.17] Huang, Guo-fu, et al. "The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration." Evidence-Based Complementary and Alternative Medicine 2014 (2014). WB ; Rabbit. PubMed:25763091 [IF=1.894] Chi et al. Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model. (2018) Med.Sci.Monit. 24:3946-3957 WB ; Rat. PubMed:29888735 [IF=1.66] Wu et al. Resveratrol induces apoptosis in SGC-7901 gastric cancer cells. (2018) Oncol.Lett. 16:2949-2956 WB ; Human. PubMed:30127883 [IF=1.652] Wang Y et al. Protective effects of astaxanthin on subarachnoid hemorrhage-induced early brain injury: Reduction of cerebral vasospasm and improvement of neuron survival and mitochondrial function.(2019)Acta Histochem. Jan;121(1):56-63. WB ; Rat. PubMed:30392635 [IF=1.63] Aksu, E. H., et al. "Mitigation of paracetamol‐induced reproductive damage by chrysin in male rats via reducing oxidative stress." Andrologia (2016). IHC-P ; Rat. PubMed:26914515 [IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082. WB ; Rat. PubMed:27665780 [IF=1.51] Sun, Y‐L., et al. "Relationship between apoptosis and proliferation in granulosa and theca cells of cystic follicles in sows." Reproduction in domestic animals 47.4 (2012): 601-608. IHC-P ; Pig. PubMed:22023076 [IF=1.39] Chen et al. Suppression effect of recombinant adenovirus vector containing hIL-24 on Hep-2 laryngeal carcinoma cells. (2014) Oncol.Let. 7:771-777 WB ; Human. PubMed:24527085 [IF=1.39] Wang et al. Human cytomegalovirus inhibits apoptosis by regulating the activating transcription factor 5 signaling pathway in human malignant glioma cells. (2014) Oncol.Let. 8:1051-1057 WB ; Human. PubMed:25120656 [IF=1.39] Chen et al. Suppression effect of recombinant adenovirus vector containing hIL-24 on Hep-2 laryngeal carcinoma cells. (2014) Oncol.Let. 7:771-777 WB ; Human. PubMed:24527085 [IF=1.39] Wang et al. Human cytomegalovirus inhibits apoptosis by regulating the activating transcription factor 5 signaling pathway in human malignant glioma cells. (2014) Oncol.Let. 8:1051-1057 WB ; Human. PubMed:25120656 [IF=1.31] Wang, Chunqiang, Wei Ma, and Yuhong Su. "NF-κB Pathway Contributes to Cadmium-Induced Apoptosis of Porcine Granulosa Cells." Biological trace element research (2013): 1-8. WB ; Pig. PubMed:23575899 [IF=1.243] Li W et al. Gallic acid caused cultured mice TM4 Sertoli cells apoptosis and necrosis. (2018) Asian-australas. J. Anim. Sci. Oct 26. WB ; Mouse. PubMed:30381745 [IF=1.14] Wang, J., et al. "Esculetin, a coumarin derivative, exerts in vitro and in vivo antiproliferative activity against hepatocellular carcinoma by initiating a mitochondrial-dependent apoptosis pathway." Brazilian Journal of Medical and Biological Research (2014): 000-000. WB ; Mouse. PubMed:25517918 [IF=1.01] Yang, H., et al. "Lipopolysaccharide-induced dental pulp cell apoptosis and the expression of Bax and Bcl-2 in vitro." Brazilian Journal of Medical and Biological Research 43.11 (2010): 1027-1033. IF(ICC) ; Human. PubMed:20945038 [IF=.88] Li, Jinglong, et al. "Deficits of learning and memory in Hemojuvelin knockout mice." Journal of Veterinary Medical Science (2015). WB ; Mouse. PubMed:26027705 [IF=.44] Sutrisno, Sutrisno, et al. "The effect of genistein on TGF-β signal, dysregulation of apoptosis, cyclooxygenase-2 pathway, and NF-kB pathway in mice peritoneum of endometriosis model." Middle East Fertility Society Journal (2017). IHC-P ; Mouse. PubMed:10.1016/j.mefs.2017.05.002 [IF=.2] Sukohar, Asep, and Mohammad Kanedi. "Mucoxin (Acetogenin) Induce Expression of Pro-Apoptosis Proteins, Bax and P53, in T47D Breast Cancer Cells." Biomedical and Pharmacology Journal 10.2 (2017): 641-649. IF(ICC) ; Human. PubMed:10.13005/bpj/1151 [IF=0] Li et al. Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis. (2015) J.Exp.Clin.Cancer.Res. 34:137 WB ; Human. PubMed:26538209 [IF=0] Zhang et al. Protective Effects of Lycium barbarum Polysaccharides on Testis Spermatogenic Injury Induced by Bisphenol A in Mice. (2014) Evid.Based.Complement.Alternat.Me. 2013:690808 IHC-P ; Mouse. PubMed:24454506 [IF=0] Ma et al. A potential adjuvant chemotherapeutics, 18β-glycyrrhetinic acid, inhibits renal tubular epithelial cells apoptosis via enhancing BMP-7 epigenetically through targeting HDAC2. (2016) Sci.Re. 6:25396 WB ; Human,Mouse. PubMed:27145860 [IF=0] Han et al. Protective effects of tao-Hong-si-wu decoction on memory impairment and hippocampal damage in animal model of vascular dementia. (2015) Evid.Based.Complement.Alternat.Me. 2015:195835 IHC-P ; Rat. PubMed:25821478 [IF=0] Xiang M et al. Biyuanling suppresses the toluene-2, 4-diisocyanate induced allergic rhinitis in guinea pigs. Oncotarget. 2017 Dec 8;9(16):12620-12629. WB&IHC-P ; Guinea pigs. PubMed:29560095 [IF=0] Gao Y et al. Hepatoprotective effects of limb ischemic post-conditioning in hepatic ischemic rat model and liver cancer patients via PI3K/ERK pathways.(2018)Int J Biol Sci. Nov 3;14(14):2037-2050. IHC ; Rat. PubMed:30585267 [IF=0] Li X et al. High level expression of ISG12 (1) promotes cell apoptosis via mitochondrial-dependent pathway and so as to hinder Newcastle disease virus replication.(2019)Vet Microbiol. Jan;228:147-156. WB&IF ; Chicken. PubMed:30593361 [IF=0] Zhang Y et al. Ginsenoside Rg3 prevents cognitive impairment by improving mitochondrial dysfunction in the rat model of Alzheimer's disease. J Agric Food Chem. 2019 Aug 27. WB&IHC-P ; Rat. PubMed:31422666 [IF=] Zou P et al. Mechanisms of Stress-Induced Spermatogenesis Impairment in Male Rats Following Unpredictable Chronic Mild Stress (uCMS). Int. J. Mol. Sci. 2019, 20, 4470. WB ; Rat. PubMed:doi:10.3390/ijms20184470 [IF=0] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. Ameliorated d-Galactose-Induced Memory Impairment, Oxidative Stress, and Neuroinflammation in Adult Rats. J Agric Food Chem. 2019 Mar 20;67(11):3140-3149. WB ; Rat. PubMed:30813721 研究领域细胞生物 信号转导 细胞凋亡 线粒体 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, 产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量21kDa细胞定位细胞浆 细胞膜 线粒体 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human Bax:84-175/192 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008].Function:Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.Subunit:Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2.Subcellular Location:Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.Isoform Beta: Cytoplasm.Isoform Gamma: Cytoplasm.Isoform Delta: Cytoplasm (Potential).Tissue Specificity:Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.Similarity:Belongs to the Bcl-2 family.SWISS:Q07812Gene ID:581Database links:Entrez Gene: 581 HumanEntrez Gene: 12028 MouseEntrez Gene: 24887 RatOmim: 600040 HumanSwissProt: Q07812 HumanSwissProt: Q07813 MouseSwissProt: Q63690 RatUnigene: 624291 HumanUnigene: 19904 MouseUnigene: 10668 RatImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.可识别分子量为21KDa的Bax蛋白，此抗体与Bax有较高特异性，且与Bc1-2及Bc1-X蛋白无交叉反应，Bax、Bc1-2和Bc1-X蛋白是凋亡调节蛋白家庭成员。与Bc1-2和Bc1-X相反，Bax蛋白的过量表达加速细胞凋亡。Bax在组织中广泛表达。Bax与Bc1-2比值的高低可用于判断恶性肿瘤耐药及复发。此抗体用于肿瘤及细胞凋亡等方面的研究。最新的研究表明：Bax可能具有肿瘤抑制作用。产品图片Sample: Brain(Rat)lysate 30ug;Liver(Mouse) lysates, 30ug;Primary: Anti-Bax (bs-0127R) at 1:200;Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000;ECL excitated the fluorescence;Predicted band size : 21kDObserved band size : 21kDSample:Cerebral cortex (Rat) Lysate at 40 ugPrimary: Anti-Bax (bs-0127R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 21 kDObserved band size: 21 kDSample:Hela-UV(Human) Cell Lysate at 30 ugPrimary: Anti-Bax (bs-0127R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 21 kDObserved band size: 23 kDSample:Hela(Human) Cell Lysate at 30 ugPrimary: Anti-Bax (bs-0127R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 21 kDObserved band size: 23 kDParaformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (bs-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (bs-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (bs-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(bs-0127R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(bs-0127R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Bax Polyclonal Antibody, Unconjugated(bs-0127R) 1:800, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bax) Polyclonal Antibody, Unconjugated (bs-0127R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Overlay histogram showing HL 60 cells stained with bs-0127R (Green line).The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (bs-0127R,1μg/1x10^6 cells) for 30 min at 22℃. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (bs- 0295G-FITC , Brillant blue line) at 1/200 dilution for 30 min at 22℃. Isotype control antibody was rabbit IgG (polyclonal,bs-0295P,Orange line) (1μg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
3-磷酸甘油醛脱氢酶（内参）单克隆抗体 | MouseAnti-GAPDH-Loading Control antibody
基本售价：980.00元
产品编号bsm-33033M英文名称GAPDH-Loading Control中文名称3-磷酸甘油醛脱氢酶（内参）单克隆抗体别 名38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10. 研究领域肿瘤 细胞生物 免疫学 神经生物学 新陈代谢 抗体来源Mouse克隆类型Monoclonal克 隆 号4F8交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Rabbit, Sheep, Hamster, Monkey, 产品应用WB=1:5000-20000 IHC-P=1:100-500 ICC=1:100 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量38kDa细胞定位细胞核 细胞浆 细胞膜 性 状Liquid浓 度1mg/ml免 疫 原Recombinded Human GAPDH : 亚 型IgG纯化方法affinity purified by Protein G储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍Loading ControlGlyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.Function:Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.Subunit:Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.Subcellular Location:Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.Post-translational modifications:S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.ISGylated (Probable).Sulfhydration at Cys-152 increases catalytic activity.Similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.SWISS:P04406Gene ID:2597Database links:Entrez Gene: 374193 ChickenEntrez Gene: 2597 HumanEntrez Gene: 100042025 MouseEntrez Gene: 14433 MouseEntrez Gene: 24383 RatEntrez Gene: 685186 RatEntrez Gene: 317743 ZebrafishOmim: 138400 HumanSwissProt: P00356 ChickenSwissProt: P04406 HumanSwissProt: P16858 MouseSwissProt: P04797 RatSwissProt: Q5XJ10 ZebrafishImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.GAPDH蛋白几乎在所有组织中都高水平表达，广泛用作Western blot蛋白质标准化的内参,是很好的内参抗体。GAPDH 作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的。在实验中，可能存在总蛋白浓度测定不准确；或者蛋白质样品在电泳前上样时产生的样品间的操作误差；这些误差需要通过测定每个样品中实际转到膜上的GAPDH的含量来进行校正，所以一般的western实验都需要进行内参设置。具体校正的方法就是将每个样品测得的目的蛋白含量与本样品的GAPDH含量相除，得到每个样品目的蛋白的相对含量。然后才进行样品与样品之间的比较。甘油醛-3-磷酸脱氢酶（Glyceraldehyde 3 phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶。除了在胞质中作为糖酵解的酶以外，有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程，包括膜融合（membrane fusion）、微管成束（microtubule bundling）、磷酸转移酶（phosphotransferase）激活、核内RNA出核、DNA复制与DNA修复。一些生理因素，诸如低氧（hypoxia）和尿糖（diabetes），可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中，以高水平持续表达。GAPDH（甘油醛-3-磷酸脱氢酶）是参与糖酵解的一种关键酶，由4个30－40kDa的亚基组成.产品图片Sample:Lane1: Skin (Mouse) Lysate at 40 ugLane2: Testis (Mouse) Lysate at 40 ugLane3: Adrenal gland (Mouse) Lysate at 40 ugLane4: Lung (Rat) Lysate at 30 ugPrimary: Anti-GAPDH (bsm-33033M) at 1/1 000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 38 kDSample:A549 Cell Lysate at 25 ug293T Cell Lysate at 40 ugPrimary: Anti-GAPDH(bsm-33033M)at 1/5000 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 38kDObserved band size: 38kDSample:H9C2 Cell (Rat) Lysate at 40 ugU87MG Cell (Human) Lysate at 40 ugHela Cell (Human) Lysate at 40 ugPrimary: Anti- GAPDH (bsm-33033M) at 1/2 000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 35 kDSample:Lane 1: Cerebrum (Rat) Lysate at 40 ugLane 2: Cerebrum (Rat) Lysate at 40 ugLane 3: Cerebrum (Rat) Lysate at 40 ugLane 4: Cerebrum (Rat) Lysate at 40 ugPrimary:Lane 1: Anti-GAPDH (bsm-33033M) at 1/2000 dilutionLane 2: Anti-GAPDH (bsm-33033M) at 1/5000 dilutionLane 3: Anti-GAPDH (bsm-33033M) at 1/10000 dilutionLane 4: Anti-GAPDH (bsm-33033M) at 1/20000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 36 kDSample:Lane 1: Hela (Human) Lysate at 40 ugLane 2: Hela (Human) Lysate at 40 ugLane 3: Hela (Human) Lysate at 40 ugLane 4: Hela (Human) Lysate at 40 ugPrimary:Lane 1: Anti-GAPDH (bsm-33033M) at 1/2000 dilutionLane 2: Anti-GAPDH (bsm-33033M) at 1/5000 dilutionLane 3: Anti-GAPDH (bsm-33033M) at 1/10000 dilutionLane 4: Anti-GAPDH (bsm-33033M) at 1/20000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 36 kDSample:Lane 1: Hela (Human) Cell Lysate at 30 ugLane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ugLane 3: Cerebrum (Mouse) Lysate at 40 ugLane 4: Cerebrum (Rat) Lysate at 40 ugLane 5: Testis (Mouse) Lysate at 40 ugLane 6: Testis (Rat) Lysate at 40 ugLane 7: Kidney (Mouse) Lysate at 40 ugLane 8: HUVEC (Human) Cell Lysate at 30 ugLane 9: A549 (Human) Cell Lysate at 30 ugLane 10: MCF-7 (Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bsm-33033M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kDSample:A431(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugJurkat(Human) Cell Lysate at 30 ugLOVO(Human) Cell Lysate at 30 ugPrimary: Anti- GAPDH (bsm-33033M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 35 kDSample:Hyperpituitarism (Mouse) Lysate at 40 ugPrimary: Anti- GAPDH (bsm-33033M) at 1/5000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 34 kDParaformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (ascites of bsm-33033M-4E8) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (ascites of bsm-33033M-4E8) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Control) Monoclonal Antibody, Unconjugated (ascites of bsm-33033M-4E8) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAPDH-Loading Control) monoclonal Antibody, Unconjugated (bsm-33033M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
3-磷酸甘油醛脱氢酶（内参）抗体 | RabbitAnti-GAPDH (Loading Control) antibody
基本售价：980.00元
产品编号bs-2188R英文名称GAPDH (Loading Control)中文名称3-磷酸甘油醛脱氢酶（内参）抗体别 名38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde 3 phosphate dehydrogenase liver; Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10. Specific References (57) | bs-2188R has been referenced in 57 publications.[IF=0] Zhang et al. Increased stathmin expression strengthens fear conditioning in epileptic rats. (2015) Biomed.Rep. 3:28-32 WB ; Rat. PubMed:25469242 [IF=7.901] Lin S et al. Strategy for hypertrophic scar therapy: Improved delivery of triamcinolone acetonide using mechanically robust tip-concentrated dissolving microneedle array. J Control Release. 2019 May 27;306:69-82. WB ; Rabbit. PubMed:31145948 [IF=7.901] Zhang N et al. Polypeptide-engineered DNA tetrahedrons for targeting treatment of colorectal cancer via apoptosis and autophagy. J Control Release. 2019 Jul 10;309:48-58. WB ; Human. PubMed:31301339 [IF=6.306] Zhou P et al. Histamine-4 receptor antagonist JNJ7777120 inhibits pro-inflammatory microglia and prevents the progression of Parkinson-like pathology and behaviour in a rat model.(2018)Brain Behav. Immun. Nov 05. WB ; Rat. PubMed:30408497 [IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93. WB ; Mouse. PubMed:30791957 [IF=5.79] Yang et al. Rab5-mediated VE-cadherin internalization regulates the barrier function of the lung microvascular endothelium. (2015) Cell.Mol.Life.Sci. 72:4849-66 WB ; Human. PubMed:26112597 [IF=5.17] Zhang et al. Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence. (2018) Oncotarget. 9:2175-2192 WB ; Rat. PubMed:29416763 [IF=5.005] Li Y et al. Exosomes derived from Toxoplasma gondii stimulate an inflammatory response through JNK signaling pathway. Nanomedicine (Lond). 2018 May;13(10):1157-1168. WB ; Human. PubMed:29542367 [IF=4.46] Yan, Lijie, et al. "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." Journal of cardiovascular pharmacology 64.4 (2014): 310-317. WB ; Human. PubMed:24887685 [IF=4.38] Sun, Pengchao, et al. "SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer." International Journal of Nanomedicine 12 (2017): 2657. WB ; Human. PubMed:28435250 [IF=4.258] Steenblock et al. The Cdc42 guanine nucleotide exchange factor FGD6 coordinates cell polarity and endosomal membrane recycling in osteoclasts. (2014) J.Biol.Che. 289:18347-59 WB ; Mouse. PubMed:24821726 [IF=3.974] Liao J et al. Inhibition of Caspase-1-dependent pyroptosis attenuates copper-induced apoptosis in chicken hepatocytes.Ecotoxicol Environ Saf. 2019 Jun 15;174:110-119. WB ; Chicken. PubMed:30822667 [IF=3.895] Shen X et al.Retinoic Acid-Induced Protein 14 (RAI14) Promotes mTOR-Mediated Inflammation Under Inflammatory Stress and Chemical Hypoxia in a U87 Glioblastoma Cell Line.(2018) Cell Mol Neurobiol. WB ; Human. PubMed:30554401 [IF=3.831] Zhang H et al. Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway.Front Pharmacol. 2019 Mar 5;10:175. WB ; Rat. PubMed:30890937 [IF=3.687] Yuan FH et al. microRNA‐30a inhibits the liver cell proliferation and promotes cell apoptosis through the JAK/STAT signaling pathway by targeting SOCS‐1 in rats with sepsis. J Cell Physiol. 2019 Apr 10. WB ; Rat. PubMed:30972748 [IF=3.562] Zhang,et al.Interferon-γ Promotes Neuronal Repair by Transplanted Neural Stem Cells in Ischemic Rats.(2018) Stem Cells and Development. 27:355-366. WB ; Rat. PubMed:29298609 [IF=3.457] Ling L et al. MicroRNA-30e promotes hepatocyte proliferation and inhibits apoptosis in cecal ligation and puncture-induced sepsis through the JAK/STAT signaling pathway by binding to FOSL2.Biomed Pharmacother. 2018 Aug;104:411-419. WB ; Rat. PubMed:29787988 [IF=3.44] Chen B et al. Inhibition of Connexin43 Hemichannels with Gap19 Protect-s Cerebral Ischemia/Reperfusion Injury via the JAK2/STAT3 Pathway in Mice.(2018) Brain Res Bull. WB ; Mouse. PubMed:30593877 [IF=3.412] Yang Q et al. Activation of Nrf2 by phloretin attenuates palmitic acid-induced endothelial cell oxidative stress via AMPK-dependent signaling.(2018) J Agric Food Chem. WB ; Human. PubMed:30525573 [IF=3.205] Liu et al. Surgical incision induces learning impairment in mice partially through inhibition of the brain-derived neurotrophic factor signaling pathway in the hippocampus and amygdala. (2018) Mol.Pain. 14:1744806918805902 WB ; Mouse. PubMed:30232930 [IF=3.144] Liu,et al.Cytoprotective effect and purification of novel antioxidant peptides from hazelnut (C. heterophylla Fisch) protein hydrolysates.(2018) Journal of Functional Foods. 42:203-215. WB ; Human. PubMed:10.1016/j.jff.2017.12.003 [IF=3.04] Ma W et al. A vanillin derivative suppresses the growth of HT29 cells through the Wnt/β-catenin signaling pathway. Eur J Pharmacol. 2019 Apr 15;849:43-49. WB ; Human. PubMed:30707959 [IF=2.78] Chai et al. Hypoxia induces pulmonary arterial fibroblast proliferation, migration, differentiation and vascular remodeling via the PI3K/Akt/p70S6K signaling pathway. (2018) Int.J.Mol.Med. 41:2461-2472 IF ; Rat. PubMed:29436587 [IF=2.669] Qiu,et al.Recombinant human maspin inhibits high glucose-induced oxidative stress and angiogenesis of human retinal microvascular endothelial cells via PI3K/AKT pathway.(2018) Molecular and Cellular Biochemistry. :. WB ; Human. PubMed:29363056 [IF=2.634] Zhang X et al. BMP9 Promotes the Extracellular Matrix of Nucleus Pulposus Cells Via Inhibition of the NotchSignaling Pathway. DNA Cell Biol. 2019 Feb 13. WB ; Human. PubMed:30758228 [IF=2.559] Qian L et al. Upregulated circular RNA circ_0074027 promotes glioblastoma cell growth and invasion by regulating miR-518a-5p/IL17RD signaling pathway. Biochem Biophys Res Commun. 2019 Mar 19;510(4):515-519. WB ; Human. PubMed:30738578 [IF=2.498] Huang H et al. Molecular characterization, expression and cellular localization of CYP17 gene during geese (Anser cygnoides) follicular development. Gene. 2018 Jun 5;658:184-190. WB ; Geese. PubMed:29544766 [IF=2.49] Wang, Hong, et al. "Osthole shows the potential to overcome P-glycoprotein‑mediated multidrug resistance in human myelogenous leukemia K562/ADM cells by inhibiting the PI3K/Akt signaling pathway." Oncology reports 35.6 (2016): 3659-3668. WB ; Human. PubMed:27109742 [IF=2.431] Wang L et al. Serious Selenium Deficiency in the Serum of Patients with Kashin–Beck Disease and the Effect of Nano-Selenium on Their Chondrocytes. Biol Trace Elem Res. 2019 Jun 8. WB ; Human. PubMed:31175635 [IF=2.387] Jiang L et al. Astrocytes induce proliferation of oligodendrocyte progenitor cells via connexin 47-mediated activation of Chi3l1 expression. Eur Rev Med Pharmacol Sci. 2019 Apr;23(7):3012-3020. WB ; Rat. PubMed:31002152 [IF=2.314] Ma H et al. Interferon-γ facilitated adjuvant-induced arthritis at early stage. Scand J Immunol. 2019 Feb 9:e12757. WB ; Mouse. PubMed:30739356 [IF=2.3] Zhang, Xiangliang, et al. "MicroRNA-216a enhances the radiosensitivity of pancreatic cancer cells by inhibiting beclin-1-mediated autophagy." Oncology Reports. WB ; PubMed:26134156 [IF=2.14] Song, Yong, et al. "Increased expression of the pluripotency markers sex-determining region Y-box 2 and Nanog homeobox in ovarian endometriosis." Reproductive Biology and Endocrinology 12.1 (2014): 42. WB ; Human. PubMed:24884521 [IF=2.11] Yan, Lijie, et al. "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." Journal of cardiovascular pharmacology 64.4 (2014): 310-317. WB ; Human. PubMed:24887685 [IF=2.11] Li et al. All-trans retinoic acid regulates the expression of the extracellular matrix protein fibulin-1 in the guinea pig sclera and human scleral fibroblasts. (2010) Mol.Vi. 16:689-97 WB ; Human. PubMed:20405022 [IF=2.09] Tan, Shifan, et al. "miR-409-3p sensitizes colon cancer cells to oxaliplatin by inhibiting Beclin-1-mediated autophagy." International Journal of Molecular Medicine. WB ; PubMed:26935807 [IF=1.922] Cao et al. Dickkopf‑3 upregulation mediates the cardioprotective effects of curcumin on chronic heart failure. (2018) Mol.Med.Rep. 17:7249-7257 WB ; rabbit. PubMed:29568962 [IF=1.92] Huang et al. Intracellular iodinated compounds affect sodium iodide symporter expression through TSH-mediated signaling pathways. (2011) Mol.Med.Rep. 4:77-80 WB ; Porcine. PubMed:21461567 [IF=1.851] Dai X et al. Silencing of MALAT1 inhibits migration and invasion by sponging miR‑1‑3p in prostate cancer cells. Mol Med Rep. 2019 Aug 22. WB ; Human. PubMed:31485645 [IF=1.632] Wang R et al. Molecular cloning and functional characterization of porcine 2',5'-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol. 2019 Mar;209:22-30. WB ; Porcine. PubMed:30885302 [IF=1.563] Wang,et al.Screening of genes involved in epithelial-mesenchymal transition and differential expression of complement-related genes induced by PAX2 in renal tubules.() Nephrology. :. WB ; Rat. PubMed:29280536 [IF=1.55] Jia, Lianqun, et al. "Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats." Molecular Medicine Reports (2016). WB ; Rat. PubMed:27082100 [IF=1.514] Wang Y et al. Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress. Iran J Basic Med Sci. 2018 Dec;21(12):1238-1244. WB ; Rat. PubMed:30627367 [IF=1.48] Hou, Yu Sen, et al. "Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein." Molecular Medicine Reports. WB ; Human. PubMed:25955291 [IF=1.358] Ma WF et al. Vanillic acid alleviates palmitic acid‐induced oxidative stress in human umbilical vein endothelial cells via Adenosine Monophosphate‐Activated Protein Kinase signaling pathway. Journal of Food Biochemistry,2019 e12893. WB ; Human. PubMed:doi:10.1111/jfbc.12893 [IF=1.28] YIN, Zhi-hong, et al. "Expression and tissue distribution analysis of Angiotensin II in sheep (Ovis aries) skins associated with white and black coat colors." Journal of Zhejiang University Science B 1 (2016). WB ; Sheep. PubMed:0 [IF=1.189] Xu Q et al. Expression of macrophage migration inhibitory factor in Aspergillus fumigatus keratitis. Int J Ophthalmol. 2019 May 18;12(5):711-716. WB ; Rat&Human. PubMed:31131227 [IF=.99] Shao, Guangcan, et al. "Construction of the plasmid coding for the expression of the EGFP‑M‑IL‑2 (88Arg, 125Ala) fusion protein and the anti‑tumor effects exerted by the fusion protein in HeLa-60 cells." Oncology Letters. WB ; Human. PubMed:26137137 [IF=0] Kaushal P et al. Ameliorative role of antioxidant supplementation on sodium-arsenite induced adverse effects on the developing rat cerebellum. Journal of Ayurveda and Integrative Medicine.2019 WB ; Rat. PubMed:doi:10.1016/j.jaim.2018.02.138 [IF=0] Wen Y et al. NADPH Oxidase Hyperactivity Contributes to Cardiac Dysfunction and Apoptosis in Rats with Severe Experimental Pancreatitis through ROS-Mediated MAPK Signaling Pathway. Oxid Med Cell Longev. 2019 May 9;2019:4578175. WB ; Rat. PubMed:31210840 [IF=0] Jiang S et al. Inhibitory Effect of Slit2-N on VEGF165-induced proliferation of vascular endothelia via Slit2-N-Robo4-Akt pathway in choroidal neovascularization. Cell Cycle. 2019 Jun;18(11):1241-1253. WB ; Human. PubMed:31081721 [IF=] Li T et al. Withanolides, extracted from Datura metel L. inhibit keratinocyte proliferation and imiquimod- induced psoriasis-like dermatitis via the STAT3/P38/ERK1/2 pathway. Molecules. 2019 Jul 17;24(14). pii: E2596. WB ; Human. PubMed:31319488 [IF=] Luo P et al. IL-37b alleviates inflammation in the temporomandibular joint cartilage via IL-1R8 pathway. Cell Prolif. 2019 Sep 27:e12692. WB ; Human. PubMed:31560411 [IF=3.266] Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019. WB ; Mouse. PubMed:31497217 [IF=2.926] Wang M et al. Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency. Glycoconj J. 2019 Sep 2. WB ; Human. PubMed:31478096 [IF=1.851] Duan X et al. Expression of TIGIT/CD155 and correlations with clinical pathological features in human hepatocellular carcinoma. Mol Med Rep. 2019 Oct;20(4):3773-3781. WB ; Human. PubMed:31485637 [IF=.607] Yang F et al. Effect of Bone Morphogenetic Protein 6 (BMP6) on Chicken Granulose Cells Proliferation and Progesterone Synthesis. Brazilian Journal of Poultry Science.2019 Feb. WB ; Chicken. PubMed:doi:10.1590/1806-9061-2018-0835 产品类型内参抗体 研究领域免疫学 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Rabbit, 产品应用WB=1:2000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量37kDa细胞定位细胞核 细胞浆 细胞膜 性 状Liquid浓 度1mg/ml免 疫 原GAPDH protein of rabbit: 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 38kDa subunits.Function:Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.Subunit:Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.Subcellular Location:Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.Post-translational modifications:S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.ISGylated (Probable).Sulfhydration at Cys-152 increases catalytic activity.Similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.SWISS:P04406Gene ID:100009074Database links:Entrez Gene: 493876 CatEntrez Gene: 374193 ChickenEntrez Gene: 403755 DogEntrez Gene: 2597 HumanEntrez Gene: 100042025 MouseEntrez Gene: 14433 MouseEntrez Gene: 396823 PigEntrez Gene: 100009074 RabbitEntrez Gene: 24383 RatEntrez Gene: 685186 RatEntrez Gene: 380259 Xenopus laevisEntrez Gene: 448356 Xenopus tropicalisEntrez Gene: 317743 ZebrafishOmim: 138400 HumanSwissProt: CatSwissProt: P00356 ChickenSwissProt: Q28259 DogSwissProt: P70685 Guinea pigSwissProt: P04406 HumanSwissProt: P16858 MouseSwissProt: P00355 PigSwissProt: P46406 RabbitSwissProt: P04797 RatSwissProt: P51469 Xenopus laevisSwissProt: Q5XJ10 ZebrafishUnigene: 544577 HumanUnigene: 592355 HumanUnigene: 598320 HumanUnigene: 304088 MouseUnigene: 309092 MouseUnigene: 317779 MouseUnigene: 343110 MouseUnigene: 392463 MouseUnigene: 392480 MouseUnigene: 414470 MouseUnigene: 458138 MouseUnigene: 458416 MouseUnigene: 475698 MouseUnigene: 129558 RatUnigene: 91450 RatUnigene: 995 Xenopus laevisUnigene: 35640 ZebrafishImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.甘油醛-3-磷酸脱氢酶（Glyceraldehyde 3 phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶。除了在胞质中作为糖酵解的酶以外，有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程，包括膜融合（membrane fusion）、微管成束（microtubule bundling）、磷酸转移酶（phosphotransferase）激活、核内RNA出核、DNA复制与DNA修复。一些生理因素，诸如低氧（hypoxia）和尿糖（diabetes），可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中，以高水平持续表达。 GAPDH（甘油醛-3-磷酸脱氢酶）是参与糖酵解的一种关键酶，由4个30－40kDa的亚基组成。产品图片Sample:U251(Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-2188R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDSample:Hela Cell (Human) Lysate at 40 ugMCF-7 Cell (Human) Lysate at 40 ugPrimary: Anti- GAPDH (bs-2188R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDSample:A549(Human) Cell Lysate at 30 ugU2os(Human) Cell Lysate at 30 ugMolt-4(Human) Cell Lysate at 30 ugA431(Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-2188R) at 1/2000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDSample:Heart (Rat) Lysate at 40 ugCerebrum (Rat) Lysate at 40 ugLiver (Mouse) Lysate at 40 ugPrimary: Anti- GAPDH (bs-2188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 35 kDSample:Liver(Rat) Lysate at 40 ugPrimary: Anti-GAPDH (bs-2188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 35 kDSample:Kidney (Mouse) Lysate at 40 ugPrimary: Anti-GAPDH (bs-2188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 35 kDSample:Muscle (Rat) Lysate at 40 ugHeart (Rat) Lysate at 40 ugPrimary: Anti- GAPDH (bs-2188R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDSample:Lane 1: NIH/3T3(Mouse) Cell Lysate at 30 ugLane 2: Cerebrum (Mouse) Lysate at 40 ugLane 3: Cerebrum (Rat) Lysate at 40 ugLane 4: HUVEC (Human) Cell Lysate at 30 ugLane 5: A549 (Human) Cell Lysate at 30 ugLane 6: MCF-7 (Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-2188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDSample:U251(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugMCF-7(Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-2188R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDParaformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated(bs-2188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-GAPDH Polyclonal Antibody, Unconjugated(bs-2188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
SARS-CoV-2 (2019-nCoV) Nucleoprotein ELISA Kit
基本售价：4800.00元
产品编号BSKV0001英文名称 SARS-CoV-2 (2019-nCoV) Nucleoprotein ELISA Kit别 名SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein; SARS-CoV-2 N ELISA Kit Specific References (1) | BSKV0001 has been referenced in 1 publications.111 [IF=4.101] Jeremy R.A. Paull. et al. Virucidal and antiviral activity of astodrimer sodium against SARS-CoV-2 in vitro. Antivir Res. 2021 Jul;191:105089 ELISA ; Human. 222线性范围0.78 - 25ng/ml应用范围S/P/CC检测限0.4 ng/ml适用样品基质cell culture supernates and cell culture exctracts保存条件Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. 为应对 COVID-19疫情的爆发，北京博奥森生物技术有限公司启动了针对新型冠状病毒的科研攻关，成功研发出SARS-CoV-2 (2019-nCoV) Nucleoprotein蛋白检测试剂盒。 该试剂盒根据ELISA双抗体夹心法原理，采用一步法，实现对2019-nCoV抗原蛋白的快速准确检测。试剂盒所采用原料均来自本公司科研团队自主研发，经严苛筛选验证，保证其灵敏度高、特异性好，结果一致且可靠，为疫情的相关疫苗及治疗性药物研究等工作贡献一份力量。产品特色：1 时间短，提高检测效率；2 操作简便，实验误差更小；3 样本需求量小，节约珍贵检测样本；4 灵敏度高及特异性好，结果一致且可靠。产品图片SARS-CoV-2 (2019-nCoV) Nucleoprotein ELISA KitExample of linearity and sensitivity of SARS-CoV-2 (2019-nCoV) Nucleoprotein ELISA Kit
3-磷酸甘油醛脱氢酶（内参）抗体 | RabbitAnti-GAPDH (Loading Control) antibody
基本售价：980.00元
产品编号bs-10900R英文名称GAPDH (Loading Control)中文名称3-磷酸甘油醛脱氢酶（内参）抗体别 名38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10. Specific References (48) | bs-10900R has been referenced in 48 publications.111 [IF=1.39] Wang, Zhi‑Liang, et al. "Irreversible electroporation‑mediated shRNA knockdown of the HPV18 E6 gene suppresses cervical cancer growth in vitro and in vivo." Oncology Letters. WB ; Human. 222PubMed:28781638111 [IF=5.008] Liang et al. Itraconazole exerts its anti-melanoma effect by suppressing Hedgehog, Wnt, and PI3K/mTOR signaling pathways. (2017) Oncotarge. 8:28510-28525 WB ; Human. 222PubMed:28212537111 [IF=5.008] Mu et al. Dickkopf-related protein 2 induces G0/G1 arrest and apoptosis through suppressing Wnt/β-catenin signaling and is frequently methylated in breast cancer. (2017) Oncotarge. 8:39443-39459 WB ; Human. 222PubMed:28467796111 [IF=4.652] Zong et al. The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro. (2016) Cell.Physiol.Bioche. 38:1100-10 WB ; Mouse. 222PubMed:26938714111 [IF=4.652] Lin et al. MiR-21 Regulates TNF-α-Induced CD40 Expression via the SIRT1-NF-κB Pathway in Renal Inner Medullary Collecting Duct Cells. (2017) Cell.Physiol.Bioche. 41:124-136 WB ; Rat. 222PubMed:28214900111 [IF=4.59] Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458 WB ; Human. 222PubMed:26881030111 [IF=2.272] Yang et al. Lrig1 is a positive prognostic marker in hepatocellular carcinoma. (2016) Onco.Targets.The. 9:7071-7079 WB ; Human. 222PubMed:27895499111 [IF=2.137] Yin et al. Expression and Clinical Significance of ILF2 in Gastric Cancer. (2017) Dis.Marker. 2017:4387081 WB ; Human. 222PubMed:28831206111 [IF=1.39] Guo et al. BRAF-activated long non-coding RNA contributes to colorectal cancer migration by inducing epithelial-mesenchymal transition. (2014) Oncol.Let. 8:869-875 WB ; Human. 222PubMed:25013510111 [IF=1.39] Wang et al. BRAF-activated long non-coding RNA contributes to cell proliferation and activates autophagy in papillary thyroid carcinoma. (2014) Oncol.Let. 8:1947-1952 WB ; Human. 222PubMed:25289082111 [IF=1.238] Liu and Xiao Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia. (2017) BMC.Ophthalmo. 17:135 WB ; Human. 222PubMed:28764685111 [IF=1.922] Chi et al. Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. (2018) Mol.Med.Rep. 17:6337-6344 WB ; Human. 222PubMed:29512784111 [IF=6.34] Zhou et al. Brd4 inhibition attenuates unilateral ureteral obstruction-induced fibrosis by blocking TGF-?-mediated Nox4 expression. (2017) Redox.Biol. 11:390-402 WB ; Rat, Human. 222PubMed:28063381111 [IF=1.77] Duan et al. Antidepressant effect of electroacupuncture regulates signal targeting in the brain and increases brain-derived neurotrophic factor levels. (2016) Neural.Regen.Res. 11:1595-1602 WB ; Rat. 222PubMed:27904490111 [IF=4.302] Jia et al. Calycosin alleviates allergic contact dermatitis by repairing epithelial tight junctions via down-regulating HIF-1α. (2018) J.Cell.Mol.Med. 22:4507-4521 WB ; Mouse. 222PubMed:29993193111 [IF=2.784] Yang et al. miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2. (2018) Int.J.Mol.Med. 42:3115-3124 WB ; PubMed:30272255111 [IF=2.766] Wan et al. Dietary protein-induced hepatic IGF-1 secretion mediated by PPARγ activation. (2017) PLoS.One. 12:e0173174 WB ; Pig. 222PubMed:28257428111 [IF=1.492] Song et al. Extracellular diffusion quantified by magnetic resonance imaging during rat C6 glioma cell progression. (2017) Braz.J.Med.Biol.Res. 50:e5407 WB ; Rat. 222PubMed:28678913111 [IF=1.936] Yu B et al. Inhibition of microRNA-143-3p attenuates myocardial hypertrophy by inhibiting inflammatory response. (2018) Nov;42(11):1584-1593. WB ; (SD) Rats. 222PubMed:30203887111 [IF=2.976] Guo H et al. Clinical associations between ASCT2 and p-mTOR in the pathogenesis and prognosis of epithelial ovarian cancer. Oncol Rep. 2018 Dec;40(6):3725-3733. WB ; Human. 222PubMed:30272366111 [IF=3.448] Liu X et al. Lysosomal dysfunction is associated with persistent lung injury in dams caused by pregnancy exposure to carbon black nanoparticles. Life Sci. 2019 Sep 15;233:116741. WB ; Mouse. 222PubMed:31398419111 [IF=2.675] Liu X et al. The lysosomal membrane protein LAMP‐2 is dispensable for PINK1/Parkin‐mediated mitophagy. FEBS Lett. 2019 Nov 6. WB ; Human. 222PubMed:31693752111 [IF=2.35] Yu B et al. Suppression of miR-143-3p contributes to the anti-fibrosis effect of atorvastatin on myocardial tissues via the modulation of Smad2 activity. Exp Mol Pathol. 2019 Nov 21;112:104346. WB ; Rat. 222PubMed:31758917111 [IF=3.598] Chang L et al. Gypenoside A protects ischemia/reperfusion injuries by suppressing miR‐143‐3p level via the activation of AMPK/Foxo1 pathway. Biofactors. 2019 Dec 30. WB ; Rat. 222PubMed:31889343111 [IF=1.851] Wang T et al. The peptide compound urantide regulates collagen metabolism in atherosclerotic rat hearts and inhibits the JAK2/STAT3 pathway. Mol Med Rep. 2020 Mar;21(3):1097-1106. WB ; Rat. 222PubMed:32016456111 [IF=4.784] Liu XQ et al. Sodium tanshinone IIA sulfonate protects against Aβ1–42-induced cellular toxicity by modulating Aβ-degrading enzymes in HT22 cells. Int J Biol Macromol. 2020 Feb 6;151:47-55. WB ; Mouse. 222PubMed:32035959111 [IF=3.448] Wang T et al. Urotensin receptor antagonist urantide improves atherosclerosis-related kidney injury by inhibiting JAK2/STAT3 signaling pathway in rats. Life Sci. 2020 Feb 13;247:117421. WB ; Rat. 222PubMed:32061865111 [IF=.578] Shen D et al. Efficacy evaluation and mechanism study on inhibition of breast cancer cell growth by multimodal targeted fluorescent nanobubbles carrying AMD070 and ICG. Nanotechnology. 2020 Mar 10;31(24):245102. WB ; human. 222PubMed:32155591111 [IF=4.268] Wu H et al. A UPLC-Q-TOF/MS-based plasma metabolomics approach reveals the mechanism of Compound Kushen Injection-based intervention against non-small cell lung cancer in Lewis tumor-bearing mice. Phytomedicine . 2020 Jun 2;76:153259. WB ; Mouse. 222PubMed:32534358111 [IF=3.647] Lin X et al. Sumoylation enhances the activity of the TGF-β/SMAD and HIF-1 signaling pathways in keloids. Life Sci . 2020 Aug 15;255:117859. WB ; Human. 222PubMed:32474020产品类型内参抗体 研究领域肿瘤 细胞生物 免疫学 信号转导 新陈代谢 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, 产品应用WB=1:10000-200000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.理论分子量38kDa细胞定位细胞核 细胞浆 细胞膜 性 状Liquid浓 度1mg/ml免 疫 原Recombinant human GAPDH full length protein 亚 型IgG纯化方法affinity purified by Protein A缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.PubMedPubMed产品介绍Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.Function:Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.Subunit:Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with EIF1AD, USP25, PRKCI and WARS.Subcellular Location:Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.Post-translational modifications:S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.ISGylated (Probable).Sulfhydration at Cys-152 increases catalytic activity.Similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.SWISS:P04406Gene ID:2597Database links:Entrez Gene: 374193 ChickenEntrez Gene: 2597 HumanEntrez Gene: 100042025 MouseEntrez Gene: 14433 MouseEntrez Gene: 24383 RatEntrez Gene: 685186 RatEntrez Gene: 317743 ZebrafishOmim: 138400 HumanSwissProt: P00356 ChickenSwissProt: P04406 HumanSwissProt: P16858 MouseSwissProt: P04797 RatSwissProt: Q5XJ10 ZebrafishGAPDH蛋白几乎在所有组织中都高水平表达，广泛用作Western blot蛋白质标准化的内参,是很好的内参抗体。GAPDH 作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的。在实验中，可能存在总蛋白浓度测定不准确；或者蛋白质样品在电泳前上样时产生的样品间的操作误差；这些误差需要通过测定每个样品中实际转到膜上的GAPDH的含量来进行校正，所以一般的western实验都需要进行内参设置。具体校正的方法就是将每个样品测得的目的蛋白含量与本样品的GAPDH含量相除，得到每个样品目的蛋白的相对含量。然后才进行样品与样品之间的比较。甘油醛-3-磷酸脱氢酶（Glyceraldehyde 3 phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶。除了在胞质中作为糖酵解的酶以外，有证据表明哺乳动物细胞中的GAPDH参与了多种胞内生化过程，包括膜融合（membrane fusion）、微管成束（microtubule bundling）、磷酸转移酶（phosphotransferase）激活、核内RNA出核、DNA复制与DNA修复。一些生理因素，诸如低氧（hypoxia）和尿糖（diabetes），可以增加GAPDH在特定细胞中的表达。GAPDH存在于几乎所有的组织中，以高水平持续表达。GAPDH（甘油醛-3-磷酸脱氢酶）是参与糖酵解的一种关键酶，由4个30－40kDa的亚基组成.产品图片Sample: 293T(human) cell lysate at 30ug;Primary:Lane1: Anti-GAPDH (bs-10900R) at 1/2000 dilutionLane2: Anti-GAPDH (bs-10900R) at 1/10000 dilutionLane3: Anti-GAPDH (bs-10900R) at 1/40000 dilutionLane4: Anti-GAPDH (bs-10900R) at 1/80000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 38kDSample:Lane 1: SiHa (Human) Cell Lysate at 30 ugLane 2: NIH/3T3(Mouse) Cell Lysate at 30 ugLane 3: Large intestine (Mouse) Lysate at 40 ugLane 4: Cerebrum (Rat) Lysate at 40 ugLane 5: Cerebrum (Mouse) Lysate at 40 ugLane 6: Testis (Rat) Lysate at 40 ugLane 7: Testis (Mouse) Lysate at 40 ugLane 8: Kidney (Mouse) Lysate at 40 ugLane 9: HUVEC (Human) Cell Lysate at 30 ugLane 10: A549 (Human) Cell Lysate at 30 ugLane 11: MCF-7 (Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-10900R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 36 kDObserved band size: 36 kDSample:293T (Human) Lysate at 40 ugPrimary:Anti-GAPDH (bs-10900R) at 1/2000~1/20000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 36 kDSample:H9C2(Rat) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-10900R) at 1/2000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 38 kDSample:293T(Human) Cell Lysate at 30 ugPrimary: Anti-GAPDH (bs-10900R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 38 kDSample:A549 Cell (Human) Lysate at 40 ugA431 Cell (Human) Lysate at 40 ugNIH/3T3 Cell (Mouse) Lysate at 40 ugPrimary: Anti-GAPDH (bs-10900R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 38 kDObserved band size: 36 kDParaformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH-Loading Contro) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-10900R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAPDH (Loading Control)) polyclonal Antibody, Unconjugated (bs-10900R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GAPDH (Loading Control)) polyclonal Antibody, Unconjugated (bs-10900R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
活化半胱胺酸蛋白酶蛋白-3抗体 | RabbitAnti-Caspase-3 antibody
基本售价：1098.00元
产品编号bs-0081R英文名称Caspase-3中文名称活化半胱胺酸蛋白酶蛋白-3抗体别 名Caspase-3 subunit p17; cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1. Specific References (117) | bs-0081R has been referenced in 117 publications.[IF=9.91] Li, Ting, et al. "Proliferation of parenchymal microglia is the main source of microgliosis after ischaemic stroke." Brain (2013): awt287. Mouse. PubMed:24154617 [IF=8.355] Chen Z et al. Enzyme-powered Janus nanomotors launched from intratumoral depots to address drug delivery barriers. Chemical Engineering Journal,2019 375, 122109. IHC ; Mouse. PubMed:doi:10.1016/j.cej.2019.122109 [IF=7.6] Teng, I., et al. "Phospholipid-functionalized mesoporous silica nanocarriers for selective photodynamic therapy of cancer." Biomaterials (2013). WB ; Mouse. PubMed:23810081 [IF=7.05] He, Nan, et al. "Tumor pH-responsive Release of Drug-conjugated Micelles from Fiber Fragments for Intratumoral Chemotherapy." ACS Applied Materials & Interfaces (2017). other ; PubMed:28876891 [IF=6.375] Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203. IHC-P + WB ; Mouse. PubMed:29317253 [IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93. WB ; Mouse. PubMed:30791957 [IF=6.01] Chen, Zhoujiang, et al. "Tunable conjugation densities of camptothecin on hyaluronic acid for tumor targeting and reduction-triggered release." Acta Biomaterialia (2016). IHC-P ; Mouse. PubMed:27424081 [IF=5.47] Pan, Bo, et al. "c-Abl Tyrosine Kinase Mediates Neurotoxic Prion Peptide-Induced Neuronal Apoptosis via Regulating Mitochondrial Homeostasis." Molecular Neurobiology (2014): 1-15. Rat. PubMed:24510275 [IF=5.23] Zhao, Yong, et al. "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." Scientific Reports 6 (2016): 37884. WB ; Pig. PubMed:27883089 [IF=5.154] Wu et al. Roles of Pannexin-1 Channels in Inflammatory Response through the TLRs/NF-Kappa B Signaling Pathway Following Experimental Subarachnoid Hemorrhage in Rats. (2017) Front.Mol.Neurosc. 10:175 WB ; Rat. PubMed:28634441 [IF=5.1] Wang, Caixia, et al. "Oral 4-(N)-stearoyl gemcitabine nanoparticles inhibit tumor growth in mouse models." Oncotarget 8.52 (2017): 89876-89886. IHC-P ; Mouse. PubMed:29163795 [IF=5.1] Wang, Caixia, et al. "Oral 4-(N)-stearoyl gemcitabine nanoparticles inhibit tumor growth in mouse models." Oncotarget 8.52 (2017): 89876-89886. IHC-P ; Mouse. PubMed:29163795 [IF=5.047] Chen Z et al. Synergistic antitumor efficacy of hybrid micelles with mitochondrial targeting and stimuli-responsive drug release. Journal of Materials Chemistry B.2019. IHC-P ; Human. PubMed: doi:10.1039/c8tb02843e [IF=5] Li, Bo, et al. "Long noncoding RNA CCAT1 functions as a ceRNA to antagonize the effect of miR-410 on the down-regulation of ITPKB in human HCT-116 and HCT-8 cells." Oncotarget 8.54 (2017): 92855. WB ; Human. PubMed:29190961 [IF=4.94] Kubatka, Peter, et al. "Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma." Journal of Cellular and Molecular Medicine (2017). IHC-P ; Rat. PubMed:28524540 [IF=4.75] Rosenzweig, Derek H., Sing J. Ou, and Thomas M. Quinn. ʺP38 mitogen‐activated protein kinase promotes dedifferentiation of primary articular chondrocytes in monolayer culture.ʺ Journal of Cellular and Molecular Medicine (2013) WB ; Bovine. PubMed:23480786 [IF=4.525] Truffi,et al.Inhibition of Fibroblast Activation Protein Restores a Balanced Extracellular Matrix and Reduces Fibrosis in Crohn's Disease Strictures Ex Vivo.(2018) Inflammatory Bowel Diseases. 24:332-345. IF(IHC-F) ; Human. PubMed:29361086 [IF=4.38] Ma, Xiangyi, et al. "DT390-triTMTP1, a novel fusion protein of diphtheria toxin with tandem repeat TMTP1 peptide, preferentially targets metastatic tumors." Molecular pharmaceutics 10.1 (2013): 115-126. IHC-P ; Human. PubMed:23198829 [IF=4.28] Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017). IHC-P ; Mouse. PubMed:29202267 [IF=4.26] Rosenzweig, Derek H., et al. "Mechanical injury of bovine cartilage explants induces depth-dependent, transient changes in MAP kinase activity associated with apoptosis." Osteoarthritis and Cartilage (2012). WB ; Bovine. PubMed:22935788 [IF=4.254] Gao L et al. Restoration of E-cadherin by PPBICA protects against cisplatin-induced acute kidney injury by attenuating inflammation and programmed cell death. Lab Invest. 2018 Jul;98(7):911-923. WB ; Mouse. PubMed:29581579 [IF=4.22] Sondag, Gregory R., et al. "Osteoactivin Induces Transdifferentiation of C2C12 Myoblasts into Osteoblasts." Journal of Cellular Physiology (2013). Mouse. PubMed:24265122 [IF=4.2] Tian, A., et al. "Nanoscale TiO 2 nanotubes govern the biological behavior of human glioma and osteosarcoma cells." International Journal of Nanomedicine10 (2015): 2377-2389. Human. PubMed:25848261 [IF=4.12] Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543 WB ; Human. PubMed:27824118 [IF=3.974] Liao J et al. Inhibition of Caspase-1-dependent pyroptosis attenuates copper-induced apoptosis in chicken hepatocytes.Ecotoxicol Environ Saf. 2019 Jun 15;174:110-119. WB ; Chicken. PubMed:30822667 [IF=3.95] Wang, Gang, et al. "Inhibition of hydrogen sulfide synthesis provides protection for severe acute pancreatitis rats via apoptosis pathway." Apoptosis (2013): 1-15. IHC-P ; Rat. PubMed:23054084 [IF=3.923] Song LL et al. TGF-β and HSP70 profiles during transformation of yak hair follicles from the anagen to catagen stage. J Cell Physiol. 2019 Feb 5. WB ; Yak. PubMed:30723905 [IF=3.92] Wei, Gang, et al. "β-Asarone inhibits neuronal apoptosis via the CaMKII/CREB/Bcl-2 signaling pathway in an in vitro model and AβPP/PS1 mice." Journal of Alzheimer's Disease 33.3 (2013): 863-880. IF(ICC) ; Mouse. PubMed:23064259 [IF=3.909] Zhu B et al. The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9. IHF ; Rat. PubMed:30851331 [IF=3.85] Wang, Yandi, et al. "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." Toxicology and Applied Pharmacology (2016). IHC-P ; Sheep. PubMed:27815134 [IF=3.84] Kubatka, Peter, et al. "Fruit peel polyphenols demonstrate substantial anti-tumour effects in the model of breast cancer." European Journal of Nutrition(2015): 1-11. IHC-P ; Rat. PubMed:25930965 [IF=3.83] Yang et al. MicroRNA-145 Increases the Apoptosis of Activated Hepatic Stellate Cells Induced by TRAIL through NF-κB Signaling Pathway. (2018) Front.Pharmacol. 8:980 WB ; Mouse. PubMed:29375381 [IF=3.82] Clark, Andrea J., et al. "WO3/Pt nanoparticles are NADPH oxidase biomimetics that mimic effector cells in vitro and in vivo." Nanotechnology27.6 (2015): 065101. PubMed:26683660 [IF=3.79] Luo, Xiaoming, et al. "Antimetastasis and antitumor efficacy promoted by sequential release of vascular disrupting and chemotherapeutic agents from electrospun fibers." International Journal of Pharmaceutics (2014). IHC-P ; Mouse. PubMed:25218185 [IF=3.687] Kubatka P et al. Anticancer Activities of Thymus vulgaris L. in Experimental Breast Carcinoma in Vivo and in Vitro. Int J Mol Sci. 2019 Apr 9;20(7). pii: E1749. IHC-P ; Rat&Mouse. PubMed:30970626 [IF=3.68] Xiao, Ke, et al. "The role of visfatin on the regulation of inflammation and apoptosis in the spleen of LPS-treated rats." Cell and Tissue Research (2014). IHC-P ; Rat. PubMed:25358398 [IF=3.53] Fang C, Zhang J, Qi D, Fan X, Luo J, et al. (2014) Evodiamine Induces G2/M Arrest and Apoptosis via Mitochondrial and Endoplasmic Reticulum Pathways in H446 and H1688 Human Small-Cell Lung Cancer Cells. PLoS ONE 9(12): e115204. WB ; Human. PubMed:25506932 [IF=3.52] Chu et al. Newcastle Disease Virus V Protein Inhibits Cell Apoptosis and Promotes Viral Replication by Targeting CacyBP/SIP. (2018) Front.Cell.Infect.Microbiol. 8:304 WB ; PubMed:30234028 [IF=3.47] Kubatka, Peter, et al. "Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model." European Journal of Nutrition (2016): 1-14. IHC-P ; Rat. PubMed:26907089 [IF=3.448] Xu B et al. Excessive mechanical stress induces chondrocyte apoptosis through TRPV4 in an anteriorcruciate ligament-transected rat osteoarthritis model. Life Sci. 2019 Jul 1;228:158-166. WB ; Rat. PubMed:31055086 [IF=3.412] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. ameliorated D-galactose-induced memory impairment, oxidative stress, and neuroinflammation in adult rats.J Agric Food Chem. 2019 Mar 20;67(11):3140-3149. WB&IHC-P ; Rat. PubMed:30813721 [IF=3.4] Gao, Hui, et al. "Hispidulin induces mitochondrial apoptosis in acute myeloid leukemia cells by targeting extracellular matrix metalloproteinase inducer."American Journal of Translational Research 8.2 (2016): 1115-1132 WB ; Human. PubMed:27158398 [IF=3.23] Wang, Yu, et al. "Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes." PloS one 12.4 (2017): e0173469. WB ; Rat. PubMed:28399139 [IF=3.17] Liu, Yang, et al. "Amelioration of Stroke-Induced Neurological Deficiency by Lyophilized Powder of Catapol and Puerarin." International Journal of Biological Sciences 10.4 (2014): 448-456. WB ; Mouse. PubMed:24719562 [IF=3.156] Andharia,et al.Electrophysiological properties of anion exchangers in the luminal membrane of guinea pig pancreatic duct cells.(2018) Pflugers Archiv: European Journal of Physiology. 470:897-907. IHC-P + WB ; Guinea Pig. PubMed:29399744 [IF=3.138] Chen J et al. Effect of artemisinin on proliferation and apoptosis-related protein expression in vivo and in vitro.Saudi J Biol Sci. 2018 Nov;25(7):1488-1493. WB&IHC ; Rat. PubMed:30505200 [IF=3.13] Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017). WB ; Human. PubMed:28887916 [IF=3.105] Yang F et al. Copper induces oxidative stress and apoptosis through mitochondria-mediated pathway in chicken hepatocytes.(2019)Toxicol In Vitro. Feb;54:310-316. WB ; Chicken. PubMed:30389602 [IF=3.06] Li, Guiying, et al. ʺGremlin Aggravates Hyperglycemia‐Induced Podocyte Injury by a TGFβ/Smad Dependent Signaling Pathway.ʺ Journal of CellularBiochemistry (2013). WB ; Mouse. PubMed:23553804 [IF=3.043] Chen Y et al. Conditional deletion of Bmp2 in cranial neural crest cells recapitulates Pierre Robin sequence in mice.(2018)Cell Tissue Res.Nov 9. IHC ; Mouse. PubMed:30413887 [IF=2.959] Song, D. et al. Ivermectin inhibits the growth of glioma cells by inducing cell cycle arrest and apoptosis in vitro and in vivo. (2018) Journal of Cellular Biochemistry. WB ; Rat. PubMed:10.1002/jcb.27420 [IF=2.903] Wang C et al. Newcastle disease virus V protein inhibits apoptosis in DF-1 cells by downregulating TXNL1. (2018) Vet Res. 49(1):102. WB ; chicken embryos. PubMed:30290847 [IF=2.9] Shan, Ming, and Ting-Jun Fan. "Cytotoxicity of carteolol to human corneal epithelial cells by inducing apoptosis via triggering the Bcl-2 family protein-mediated mitochondrial pro-apoptotic pathway." Toxicology in Vitro (2016). ELISA ; Human. PubMed:27216471 [IF=2.86] Liu, Yaping, et al. "Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice." International Journal of Molecular Sciences 17.1 (2016): 96. WB ; Mouse. PubMed:26771609 [IF=2.85] Xu et al. Resveratrol controlled the fate of porcine pancreatic stem cells through the Wnt/β-catenin signaling pathway mediated by Sirt1. (2017) PLoS.On. 12:e0187159 WB ; Pig. PubMed:29073244 [IF=2.791] Wang Q et al. Cdc20 and molecular chaperone CCT2 and CCT5 are required for the Muscovy duck reovirus p10. 8-induced cell cycle arrest and apoptosis. Vet Microbiol. 2019 Jun 28;235:151-163. WB ; Monkey. PubMed:31282373 [IF=2.75] Ye, Bengui, et al. "Anti-tumor activity and relative mechanism of ethanolic extract of Marsdenia tenacissima (Asclepiadaceae) against human hematologic neoplasm in vitro and in vivo." Journal of Ethnopharmacology (2014). WB ; Human. PubMed:24583069 [IF=2.7] Lv, Runxiao, et al. "Neuroprotective effect of allicin in a rat model of acute spinal cord injury." Life Sciences (2015). WB ; Rat. PubMed:26546416 [IF=2.69] Lin, Caiyu, et al. "Lithocarpus Polystachyus Rehd Leaf Aqueous Extract Inhibits Human Breast Cancer Growth In Vitro and In Vivo." Nutrition and Cancer (2014). WB ; Human. PubMed:24660968 [IF=2.656] Liu et al. Effects of insulin-like growth factor binding protein 3 on apoptosis of cutaneous squamous cell carcinoma cells. (2018) Onco.Targets.Ther. 11:6569-6577 WB ; PubMed:30323629 [IF=2.61] Lei, Mingxing, et al. ʺGsdma3 is a new factor needed for TNF-α-mediated apoptosis signal pathway in mouse skin keratinocytes.ʺ Histochemistry and cell biology 138.3 (2012): 385-396. WB, IHC-P ; Mouse. PubMed: 22585037 [IF=2.561] Peng X et al. Overexpression of modified human TRβ1 suppresses the growth of hepatocarcinoma SK-hep1 cells in vitro and in xenograft models.Mol Cell Biochem. 2018 Dec;449(1-2):207-218. IHC-P&WB ; Mouse&Human. PubMed:29679278 [IF=2.535] Chen J et al. Tension induces intervertebral disc degeneration via endoplasmic reticulum stress-mediated autophagy. Biosci Rep. 2019 Jul 8. pii: BSR20190578. WB&IHC ; Rat. PubMed:31285389 [IF=2.53] Yoshinaga, Ayana, et al. "NEU3 inhibitory effect of naringin suppresses cancer cell growth by attenuation of EGFR signaling through GM3 ganglioside accumulation." European Journal of Pharmacology (2016). WB ; Human. PubMed:27105818 [IF=2.52] Yao, Kun, et al. "Knockdown of RLIP76 expression by RNA interference inhibits proliferation, enhances apoptosis, and increases chemosensitivity to daunorubicin in U937 leukemia cells." Tumor Biology (2014): 1-9. WB ; Human. PubMed:24839008 [IF=2.498] Du J et al. MicroRNA-204-5p regulates 3T3-L1 preadipocyte proliferation, apoptosis and differentiation.Gene. 2018 Aug 20;668:1-7. ICF ; Mouse. PubMed:29775748 [IF=2.491] Şahin S et al. Vitamin D protects against hippocampal apoptosis related with seizures induced by kainic acid and pentylenetetrazol in rats.(2018) Epilepsy Res.149 IHC-P ; Rat . PubMed:30584976 [IF=2.41] Guan, Junhong, et al. "Bone morphogenetic protein-7 (BMP-7) mediates ischemic preconditioning-induced ischemic tolerance via attenuating apoptosis in rat brain." Biochemical and Biophysical Research Communications (2013). WB ; Rat. PubMed:24184479 [IF=2.38] Gao, Hui, et al. "Hispidulin Potentiates the Antitumor Effect of Sunitinib Against Human Renal Cell Carcinoma in Laboratory Models." Cell Biochemistry and Biophysics: 1-8. WB ; Human. PubMed:25260394 [IF=2.38] Gao, Hui, et al. "shRNA-Mediated EMMPRIN Silencing Inhibits Human Leukemic Monocyte Lymphoma U937 Cell Proliferation and Increases Chemosensitivity to Adriamycin." Cell Biochemistry and Biophysics: 1-9. WB ; Human. PubMed:25260396 [IF=2.38] Abdel-Hamid, Nagwa I., Mona F. El-Azab, and Yasser M. Moustafa. "Macrolide antibiotics differentially influence human HepG2 cytotoxicity and modulate intrinsic/extrinsic apoptotic pathways in rat hepatocellular carcinoma model." Naunyn-Schmiedeberg's Archives of Pharmacology (2017): 1-17. IHC-P ; Rat. PubMed:28070612 [IF=2.37] Liu, Jun-Song, et al. "β-elemene enhances the radiosensitivity of gastric cancer cells by inhibiting Pak1 activation." World J Gastroenterol 21.34 (2015): 9945-9956. WB ; Human. PubMed:26379399 [IF=2.33] Yang, Juan, Guiyuan Li, and Keqiang Zhang. "Pro-survival effects by NF-κB, Akt and ERK (1/2) and anti-apoptosis actions by Six1 disrupt apoptotic functions of TRAIL-Dr4/5 pathway in ovarian cancer." Biomedicine & Pharmacotherapy 84 (2016): 1078-1087. WB ; Human. PubMed:27780136 [IF=2.31] Dong, Liwei, et al. "Echinacoside induces apoptotic cancer cell death by inhibiting the nucleotide pool sanitizing enzyme MTH1." OncoTargets and Therapy 8 (2015): 3649. Human. PubMed:26677335 [IF=2.27] Li, M., et al. ʺmiR‐34c works downstream of p53 leading to dairy goat male germline stem‐cell (mGSCs) apoptosis.ʺ Cell Proliferation 46.2 (2013): 223-231. WB ; Goat. PubMed:23510477 [IF=2.15] Tang, Xi-Lan, et al. "The Cardioprotective Effect of Protocatechuic Acid on Myocardial Ischemia/Reperfusion Injury." Journal of Pharmacological Sciences 125.2 (2014): 176-183. WB ; Rat. PubMed:24942213 [IF=2.13] Cetintas, Vildan Bozok, et al. "Effects of flavopiridol on critical regulation pathways of CD133high/CD44high lung cancer stem cells." Medicine 95.43 (2016): e5150. IF(ICC) ; Mouse. PubMed:27787370 [IF=2.1] Zhang, Beiru, et al. "The Toxicity Mechanisms of Action of Aβ25–35 in Isolated Rat Cardiac Myocytes." Molecules 19.8 (2014): 12242-12257. WB ; Rat. PubMed:25123188 [IF=2.1] Hu, Hanhua, et al. "Drug-induced apoptosis of Echinococcus granulosus protoscoleces." Parasitology research 109.2 (2011): 453-459. IHC-P ; Others. PubMed:21365454 [IF=2.088] Wu LY, et al. Biochanin A Reduces Inflammatory Injury and Neuronal Apoptosis following Subarachnoid Hemorrhage via Suppression of the TLRs/TIRAP/MyD88/NF-B Pathway. Behav Neurol. 2018 Jun 3;2018:1960106. WB ; Rat. PubMed:29971136 [IF=2.03] Aksu, Ilkay, et al. "Anxiety correlates to decreased blood and prefrontal cortex IGF-1 levels in streptozotocin induced diabetes." Neuroscience Letters (2012). Rat. PubMed:23123774 [IF=1.949] Li Q et al. The effects of photobiomodulation therapy on mouse pre-osteoblast cell line MC3T3-E1 proliferation and apoptosis via miR-503/Wnt3a pathway.(2018) Lasers Med Sci. Sep 15 WB ; mouse. PubMed:30218348 [IF=1.894] An N et al. Involvement of Activation of Mitogen-Activated Protein Kinase (MAPK)/Extracellular Signal-Regulated Kinase (ERK) Signaling Pathway in Proliferation of Urethral Plate Fibroblasts in Finasteride-Induced Rat Hypospadias.(2018) Med Sci Monit. WB ; Rat . PubMed:30538214 [IF=1.871] Huang XW et al. Scutellarein suppresses Aβ‑induced memory impairment via inhibition of the NF‑κB pathway in vivo and in vitro. Oncol Lett. 2019 Jun;17(6):5581-5589. WB ; Rat. PubMed:31186780 [IF=1.85] Li, Mingzhao, et al. ʺExpression of miR‐34c in response to overexpression of Boule and Stra8 in dairy goat male germ line stem cells (mGSCs).ʺ Cell Biochemistry and Function (2013). Goat. PubMed:23508548 [IF=1.83] KASSAYOVÁ, MONIKA, et al. "Anticancer and Immunomodulatory Effects of Lactobacillus plantarum LS/07, Inulin and Melatonin in NMU-induced Rat Model of Breast Cancer." Anticancer Research 36.6 (2016): 2719-2728. IHC-P ; Rat. PubMed:27272781 [IF=1.77] Sun et al. Protective effects of ginsenoside Rg1 against hydrogen peroxide-induced injury in human neuroblastoma cells. (2016) Neural.Regen.Re. 11:1159-64 IF(ICC) ; Human. PubMed:27630703 [IF=1.77] Sun et al. Protective effects of ginsenoside Rg1 against hydrogen peroxide-induced injury in human neuroblastoma cells. (2016) Neural.Regen.Re. 11:1159-64 IF(ICC) ; Human. PubMed:27630703 [IF=1.72] Zhao, Jun, et al. "The cytotoxic and pro-apoptotic effects of phenylephrine on corneal stromal cells via a mitochondrion-dependent pathway both in vitro and in vivo." Experimental and Toxicologic Pathology (2016). ELISA ; Human. PubMed:27344612 [IF=1.701] Kosutova P et al. Reduction of Lung Inflammation, Oxidative Stress and Apoptosis by the PDE4 Inhibitor Roflumilast in Experimental Model of Acute Lung Injury. Physiol Res. 2018 Dec 31;67(Supplementum 4):S645-S654. IHC ; Rabbit. PubMed:30607971 [IF=1.69] Song et al. Anti-autophagic and anti-apoptotic effects of memantine in a SH-SY5Y cell model of Alzheimer's disease via mammalian target of rapamycin-dependent and -independent pathways. (2015) Mol.Med.Re. 12:7615-22 WB ; Human. PubMed:26459718 [IF=1.64] Kosutova, P., et al. "Intravenous dexamethasone attenuated inflammation and influenced apoptosis of lung cells in an experimental model of acute lung injury." Physiological research 65.Supplementum 5 (2016): S663. IHC-P ; Rabbit. PubMed:28006948 [IF=1.616] Wang YY et al. GABA regulates the proliferation and apoptosis of MAC-T cells through the LPS-induced TLR4 signaling pathway.Res Vet Sci. 2018 Jun;118:395-402. WB ; Bovine. PubMed:29684816 [IF=1.56] Qu, Zhongyuan, et al. "Chelidonine induces mitotic slippage and apoptotic-like death in SGC-7901 human gastric carcinoma cells." Molecular medicine reports 13.2 (2016): 1336-1344. WB ; Human. PubMed:26677104 [IF=1.55] Jia, Jianping, et al. "A preliminary study of the effect of ECRG4 overexpression on the proliferation and apoptosis of human laryngeal cancer cells and the underlying mechanisms." Molecular Medicine Reports. Human. PubMed:26165988 [IF=1.55] Song, Guijun, et al. "Anti-autophagic and anti-apoptotic effects of memantine in a SH-SY5Y cell model of Alzheimers disease via mammalian target of rapamycin-dependent and-independent pathways." Molecular Medicine Reports. WB ; Human. PubMed:not posted yet [IF=1.55] Ding, Qing, et al. "8‑bromo‑7‑methoxychrysin induces apoptosis by regulating Akt/FOXO3a pathway in cisplatin‑sensitive and resistant ovarian cancer cells." Molecular medicine reports 12.4 (2015): 5100-5108. WB ; Human. PubMed:26151347 [IF=1.55] Han, Ning, et al. "Agmatine protects Müller cells from high‑concentration glucose‑induced cell damage via N‑methyl‑D‑aspartic acid receptor inhibition." Molecular medicine reports 12.1 (2015): 1098-1106. WB ; Rat. PubMed:25816073 [IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082. WB ; Rat. PubMed:27665780 [IF=1.51] Sun, Y‐L., et al. "Relationship between apoptosis and proliferation in granulosa and theca cells of cystic follicles in sows." Reproduction in domestic animals 47.4 (2012): 601-608. IHC-P ; Pig. PubMed:22023076 [IF=1.48] Liu, Yong‑Chao, et al. "RNAi-mediated gene silencing of vascular endothelial growth factor C suppresses growth and induces apoptosis in mouse breast cancer in vitro and in vivo." Oncology Letters. (2016) WB ; Mouse. PubMed:27666771 [IF=1.41] Song et al. Effects of HSYA on the proliferation and apoptosis of MSCs exposed to hypoxic and serum deprivation conditions. (2018) Exp.Ther.Med. 15:5251-5260 WB ; Rat. PubMed:29904409 [IF=1.243] Li W et al. Gallic acid caused cultured mice TM4 Sertoli cells apoptosis and necrosis. (2018) Asian-australas. J. Anim. Sci. Oct 26. WB ; Mouse. PubMed:30381745 [IF=1.14] Wang, J., et al. "Esculetin, a coumarin derivative, exerts in vitro and in vivo antiproliferative activity against hepatocellular carcinoma by initiating a mitochondrial-dependent apoptosis pathway." Brazilian Journal of Medical and Biological Research (2014): 000-000. WB ; Mouse. PubMed:25517918 [IF=1.06] Zhang, Xiaolin, and Hao Yu. "Matrine inhibits diethylnitrosamine-induced HCC proliferation in rats through inducing apoptosis via p53, Bax-dependent caspase-3 activation pathway and down-regulating MLCK overexpression (Supplement 2016)." Iranian Journal of Pharmaceutical Research (2016). WB ; Rat. PubMed:27642320 [IF=.87] Ochigbo, Grace Onyeche, et al. "Polyphenol-rich fraction of Parquetina nigrescens mitigates dichlorvos-induced neurotoxicity and apoptosis." Journal of Ayurveda and Integrative Medicine (2017). Rat. PubMed:28256303 [IF=0] Li et al. Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis. (2015) J.Exp.Clin.Cancer.Res. 34:137 WB ; Human. PubMed:26538209 [IF=0] Wang, Yu, et al. "Tanshinone II A Relieves Adriamycin-induced Myocardial Injury in Rat Model." International Journal of Chemistry 8.1 (2016): 40. WB ; Rat. PubMed:10.5539/ijc.v8n1p40 [IF=0] Petty, Howard R. "NANOPARTICLE THERAPY IN CANCER." U.S. Patent No. 20,150,335,744. 26 Nov. 2015. IF(ICC) ; PubMed:1111 [IF=0] Xia et al. Reversion of p-glycoprotein-mediated multidrug resistance in human leukemic cell line by diallyl trisulfide. (2012) Evid.Based.Complement.Alternat.Me. 2012:719805 WB ; Human. PubMed:22919419 [IF=0] Tang et al. The cardioprotective effects of citric Acid and L-malic Acid on myocardial ischemia/reperfusion injury. (2013) Evid.Based.Complement.Alternat.Me. 2013:820695 WB ; Rat. PubMed:23737849 [IF=0] Zhou et al. Suppression of E. multilocularis hydatid cysts after ionizing radiation exposure. (2013) PLoS.Negl.Trop.Di. 7:e2518 WB ; Mouse. PubMed:24205427 [IF=0] Zhong et al. MicroRNA-200a inhibits epithelial-mesenchymal transition in human hepatocellular carcinoma cell line. (2015) Int.J.Clin.Exp.Patho. 8:9922-31 WB ; Human. PubMed:26617701 [IF=0] Pang and Fan Cytotoxic effect and possible mechanisms of Tetracaine on human corneal epithelial cells in vitro. (2016) Int.J.Ophthalmo. 9:497-504 ELISA ; Human. PubMed:27162719 [IF=0] Li X et al. High level expression of ISG12 (1) promotes cell apoptosis via mitochondrial-dependent pathway and so as to hinder Newcastle disease virus replication.(2019)Vet Microbiol. Jan;228:147-156. WB ; Chicken. PubMed:30593361 [IF=.181] Wang S et al. Insulin-like growth factor-1 inhibits the expression of autophagic ID1 in cerebrovascular endothelial cells. Int J Clin Exp Med 2019;12(8):10486-10493. WB ; Human. PubMed:ISSN:1940-5901/IJCEM0091146 [IF=0] Chen S et al. Anthocyanins from Lycium ruthenicum Murr. Ameliorated d-Galactose-Induced Memory Impairment, Oxidative Stress, and Neuroinflammation in Adult Rats. J Agric Food Chem. 2019 Mar 20;67(11):3140-3149. WB ; Rat. PubMed:30813721 研究领域肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 Alzheimer's 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, 产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg/Test ICC=1:100 IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量28kDa细胞定位细胞浆 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human caspase-3 p17 subunit:1-100/277 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.Reacts with Caspase-3 subunit p17 and precursor.Function:Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.Subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce.Subcellular Location:Cytoplasm.Tissue Specificity:Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.Post-translational modifications:Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.Similarity:Belongs to the peptidase C14A family.SWISS:P42574Gene ID:836Database links:Entrez Gene: 836 HumanEntrez Gene: 12367 MouseEntrez Gene: 397244 PigEntrez Gene: 100008840 RabbitEntrez Gene: 25402 RatOmim: 600636 HumanSwissProt: P42574 HumanSwissProt: P70677 MouseSwissProt: Q95ND5 PigSwissProt: Q8MJC3 RabbitSwissProt: P55213 RatUnigene: 141125 HumanUnigene: 34405 MouseUnigene: 10562 RatImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.Caspase3广泛分布于各种不同类型的细胞中，是Caspase家族中最重要的凋亡执行者之一，激活的Caspase-3能使许多与细胞结构、细胞周期及DNA修复等相关蛋白或激酶失活，从而使细胞凋亡.产品图片Sample:Lane 1: Spleen (Mouse) Lysate at 40 ugLane 2: Lung (Mouse) Lysate at 40 ugLane 3: Lymph node (Mouse) Lysate at 40 ugLane 4: Cerebrum (Mouse) Lysate at 40 ugLane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ugLane 6: Spleen (Rat) Lysate at 40 ugLane 7: Lung (Rat) Lysate at 40 ugLane 8: Lymph node (Rat) Lysate at 40 ugLane 9: Cerebrum (Rat) Lysate at 40 ugLane 10: Hela (Human) Cell Lysate at 30 ugPrimary: Anti-Caspase-3 (bs-0081R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 35 kDObserved band size: 37 kDSample:Lane 1: Raji (Human) Cell Lysate at 30 ugLane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ugLane 3: Lung (Mouse) Lysate at 40 ugLane 4: Lung (Rat) Lysate at 40 ugPrimary: Anti-Caspase-3 (bs-0081R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 35 kDObserved band size: 37 kDSample:Kidney (Mouse) Lysate at 40 ugPrimary: Anti-Caspase-3 (bs-0081R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 28 kDObserved band size: 17 kDSample:Liver (Mouse) Lysate at 40 ugPrimary: Anti-Caspase-3 (bs-0081R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 28 kDObserved band size: 28 kDSample: Raji Cell lysate;Primary: Anti-Caspase-3 (bs-0081R) at 1:300;Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;Predicted band size: 17/28 kDObserved band size: 26 kDSample:Liver(Sheep)Lysate at 40 ugPrimary: Anti-Caspase-3(bs-0081R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 28kDObserved band size: 30kDSample:Lung(Mouse) Lysate at 40 ugHela(Human) Cell Lysate at 30 ugNIH/3T3(Mouse) Cell Lysate at 30 ugPrimary: Anti-Caspase-3 (bs-0081R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 35/29/19/17 kDObserved band size: 38 kDProtein:Lung(Mouse) lysate at 30ug;Primary: Anti-caspase-3 (bs-0081R) at 1:300 dilution;Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 5000;Predicted band size : 17/28 kDObserved band size : 28 kDTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Caspase-3 Polyclonal Antibody, Unconjugated(bs-0081R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: rabbit pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-Caspase-3 Polyclonal Antibody, Unconjugated(bs-0081R) 1:300, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase-3) polyclonal Antibody, Unconjugated (bs-0081R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.The figure annotation:The blue histogram is unstained cells. The Orange histogram is cells stained with Rabbit IgG/FITC (bs-0295P-FITC).The green histogram is cells stained with Rabbit Anti-Caspase-3/FITC Conjugated antibody (bs-0081R-FITC).ControlsPositive control: HepG 2 cellsIsotype control: Cell lines treated with Rabbit IgG/FITC (bs-0295P-FITC) instead of the primary antibody to confirm that primary antibody binding is specific. 2μg in 1 00μL1 X PBS containing 0.5% BSA.The figure annotation:The blue histogram is unstained cells.The Orange histogram is cells stained with Rabbit IgG/FITC (bs-0295P-FITC).The green histogram is cells stained with Rabbit Anti-Caspase-3/FITC Conjugated antibody (bs-0081R-FITC).ControlsPositive control: A549 cellsIsotype control: Cell lines treated with Rabbit IgG/FITC(bs-0295P-FITC) instead of the primary antibody to confirm that primary antibody binding is specific. 3μg in 1 00 μL 1 X PBS containing 0.5% BSA.Blank control:Mouse spleen.Primary Antibody (green line): Rabbit Anti-Caspase-3 antibody (bs-0081R)Dilution: 2μg /10^6 cells;Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody : Goat anti-rabbit IgG-AF488Dilution: 1μg /test.ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
核心结合因子α1/成骨特异性转录因子/Cbfα1抗体 | RabbitAnti-RUNX2 antibody
基本售价：1098.00元
产品编号bs-1134R英文名称RUNX2中文名称核心结合因子α1/成骨特异性转录因子/Cbfα1抗体别 名RUNX2_HUMAN; Runt-related Transcription Factor 2; CBF alpha 1; CBF-alpha-1; PEBP2-alpha A; CBFA1; CCD; CCD1; Cleidocranial dysplasia 1; Core binding factor; Core binding factor runt domain alpha subunit 1; Core binding factor subunit alpha 1; MGC120023; Oncogene AML 3; OSF 2; OSF2; OSF-2; Osteoblast specific transcription factor 2; OTTHUMP00000016533; PEA2 alpha A; PEA2aA; PEBP2 alpha A; PEBP2A1; PEBP2A2; PEBP2aA1; Polyomavirus enhancer binding protein 2 alpha A subunit; Runt domain; Runt related transcription factor 2; SL3 3 enhancer factor 1 alpha A subunit; SL3/AKV core binding factor alpha A subunit; AML3; CLCD. Specific References (10) | bs-1134R has been referenced in 10 publications.[IF=4.095] Gao K et al. Regulation and function of runt-related transcription factors (RUNX1 and RUNX2) in goat granulosa cells.J Steroid Biochem Mol Biol. 2018 Jul;181:98-108. IHC-P ; Goat. PubMed:29626608 [IF=3.68] Zhang, Ping, et al. "Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bones." Cell and Tissue Research 350.3 (2012): 425-437. WB ; Rat. PubMed:22237862 [IF=3.414] Lu H et al. Comparative evaluation of book‐type acellular bone scaffold and fibrocartilage scaffold for bone‐tendon healing. J Orthop Res. 2019 Apr 12. ICF ; Rabbit. PubMed:30977542 [IF=2.784] Chen X et al. Modulation of proliferation and differentiation of gingiva‑derived mesenchymal stem cells by concentrated growth factors: Potential implications in tissue engineering for dental regeneration and repair. Send to Int J Mol Med. 2019 Apr 24. WB ; Human. PubMed:31017269 [IF=2.127] Xu Y et al. Silver nanoparticles promote osteogenic differentiation of human periodontal ligamentfibroblasts by regulating the RhoA-TAZ axis. Cell Biol Int. 2019 May 22. WB ; Human. PubMed:31115946 [IF=1.892] Li CL et al. Preparation and In Vivo Expression of CS-PEI/pCGRP Complex for Promoting Fracture Healing. International Journal of Polymer Science.2019 9432194. WB ; Rat. PubMed:doi:10.1155/2019/9432194 [IF=1.872] Li Z et al. Angiogenesis and bone regeneration by allogeneic mesenchymal stem cell intravenous transplantation in rabbit model of avascular necrotic femoral head. J Surg Res. 2013 Jul;183(1):193-203. IHC-P ; Rabbit. PubMed:23290592 [IF=1.56] Li, Pengcui, et al. "Blockade of hypoxia-induced CXCR4 with AMD3100 inhibits production of OA-associated catabolic mediators IL-1β and MMP-13." Molecular Medicine Reports. WB ; Human. PubMed:27356492 [IF=1.26] Sun et al. The role of inhibition by phosphocitrate and its analogue in chondrocyte differentiation and subchondral bone advance in Hartley guinea pigs. (2018) Exp.Ther.Med. 15:3320-3328 IHC-P ; Guinea Pig. PubMed:29545850 [IF=1.2] Hu, Fei, et al. "High expression of periostin is dramatically associated with metastatic potential and poor prognosis of patients with osteosarcoma." World Journal of Surgical Oncology 12.1 (2014): 287. IHC-P ; Human. PubMed:25224568 研究领域干细胞 转录调节因子 表观遗传学 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, 产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分 子 量57(hu)/67(mo,ratkDa细胞定位细胞核 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human RUNX2:202-300/521 亚 型IgG纯化方法affinity purified by Protein A储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed 产品介绍This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2008].Function:Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. [SUBUNIT] Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors (By similarity). Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 (By similarity). The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with MYST3 and MYST4.Subunit:Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with G22P1 (Ku70) and XRCC5 (Ku80). Interacts with MYST3 and MYST4.Subcellular Location:Nucleus.Tissue Specificity:Specifically expressed in osteoblasts.Post-translational modifications:Phosphorylated; probably by MAP kinases (MAPK). Isoform 3 is phosphorylated on Ser340.DISEASE:Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies.Similarity:Contains 1 Runt domain.SWISS:Q13950Gene ID:860Database links:Entrez Gene: 860 HumanEntrez Gene: 12393 MouseEntrez Gene: 100155806 PigEntrez Gene: 367218 RatOmim: 600211 HumanSwissProt: Q13950 HumanSwissProt: Q9XSB7 HorseSwissProt: Q08775 MouseSwissProt: Q9Z2J9 RatUnigene: 535845 HumanUnigene: 391013 MouseUnigene: 391017 MouseUnigene: 214214 RatUnigene: 83672 RatImportant Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.RUNX2又称：Cbfα1(Core-binding factor, alpha 3 subunit) 是新发现的一类调控间充质干细胞向成骨方向分化的特异性转录因子,参与骨形成,骨骼生长和发育的一类重要细胞,它起源于多能间充质干细胞,是间充质干细胞在体内的各种调控因素的调节下发育而成的。产品图片Sample:Cerebrum (mouse) Lysate at 40 ugCerebrum (Rat) Lysate at 40 ugHeart (mouse) Lysate at 40 ugHeart (Rat) Lysate at 40 ugPrimary: Anti- RUNX2 (bs-1134R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 57/67 kDObserved band size: 57 kDSample:HL-60(human)Cell Lysate at 40 ugTestis (mouse)l Lysate at 40 ugPrimary: Anti- RUNX2 (bs-1134R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 57/67 kDObserved band size: 57 kDParaformaldehyde-fixed, paraffin embedded (Mouse thyroid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RUNX2) Polyclonal Antibody, Unconjugated (bs-1134R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control:HL-60.Primary Antibody (green line): Rabbit Anti-RUNX2 antibody (bs-1134R)Dilution: 1μg /10^6 cells;Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody : Goat anti-rabbit IgG-AF488Dilution: 1μg /test.ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
转化生长因子β1/TGF β1/TGF-β1抗体 | RabbitAnti-TGF beta 1 antibody
基本售价：1098.00元
产品编号bs-0086R英文名称TGF beta 1中文名称转化生长因子β1/TGF β1/TGF-β1抗体别 名CED; DPD1; TGF beta 1; TGF beta; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor, beta 1. Specific References (84) | bs-0086R has been referenced in 84 publications.111 [IF=1.96] Kou, Wei, et al. ʺTransforming growth factor-β1 promotes Treg commitment in nasal polyposis after intranasal steroid treatment.ʺ Inflammation Research (2013): 1-7. ELISA ; Human. 222111 [IF=4.52] Abdelmagid, Samir M., et al. "Mutation in Osteoactivin Reduces Bone Formation in Vivo and Osteoblast Differentiation in Vitro." The American Journal of Pathology (2014). WB ; Rat. 222111 [IF=1.06] Erbas, O., et al. "Nephro-protective effect of granulocyte colony-stimulating factor in streptozotocin induced diabetic rats." Biotechnic & Histochemistry 0 (2014): 1-9. IHC-P ; Rat. 222111 [IF=.94] Tang, Jin, et al. "Increased adenosine levels contribute to ischemic kidney fibrosis in the unilateral ureteral obstruction model." Experimental and Therapeutic Medicine. IHC-P ; Mouse. 222111 [IF=3.23] Xue X, Chen Q, Zhao G, Zhao J-Y, Duan Z, Zheng P-S (2015) The Overexpression of TGF-β and CCN2 in Intrauterine Adhesions Involves the NF-κB Signaling Pathway. PLoS ONE 10(12): e0146159. IHC-P ; Human. 222111 [IF=7.39] Ganguly, Rituparna, et al. "Anti-atherogenic Effect of Trivalent Chromium-loaded CPMV Nanoparticles in Human Aortic Smooth Muscle Cells under Hyperglycemic Conditions in vitro." Nanoscale (2016). WB ; Human. 222111 [IF=1.88] Venter, Neil Grant, et al. "Use of platelet-rich plasma in deep second-and third-degree burns." Burns (2016). other ; 111 [IF=1.64] Eren, Kenan, et al. "The Suppression of Wound Healing Response with Sirolimus and Sunitinib Following Experimental Trabeculectomy in a Rabbit Model." Current Eye Research (2016): 1-10. IHC-P ; Rabbit. 222111 [IF=1.89] Hu, Jianguo, Biao Zeng, and Xingwei Jiang. "The expression of marker for endometrial stem cell and fibrosis was increased in intrauterine adhesious."International journal of clinical and experimental pathology 8.2 (2015): 1525. IHC-P ; Mouse. 222111 [IF=1.27] Sun, Zhenqiang, et al. "Immunological effect induced by mesenchymal stem cells in a rat liver transplantation model." Experimental and therapeutic medicine 10.2 (2015): 401-406. IHC-P ; Rat. 222111 [IF=1.4] Zhu, Yi, et al. "High Molecular Weight Hyaluronic Acid Inhibits Fibrosis of Endometrium." Medical Science Monitor 22 (2016): 3438-3445. IHC-P ; Mouse. 222111 [IF=5.29] Shang, Peijin, et al. "Acetyl-11-Keto-β-Boswellic Acid Attenuates Prooxidant and Profibrotic Mechanisms Involving Transforming Growth Factor-β1, and Improves Vascular Remodeling in Spontaneously Hypertensive Rats." Scientific Reports 6 (2016): 39809. IHC-P ; Rat. 222111 [IF=1.56] Du, Jinghua, et al. "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." Molecular medicine reports 13.3 (2016): 2159-2166. WB ; Mouse. 222111 [IF=2.55] Shen, Haitao, et al. "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." International Immunopharmacology 46 (2017): 16-22. IHC-P ; Mouse. 222111 [IF=2.13] Mao, Rui, et al. "Efficacy of radiotherapy for the treatment of cystic echinococcosis in naturally infected sheep." Infectious Diseases of Poverty 6.1 (2017): 88. IHC-P ; Sheep. 222111 [IF=2.45] Xiu, Zhi-Ming, et al. "1-Acetyl-5-phenyl-1H-pyrrol-3-ylacetate: an aldose reductase inhibitor for the treatment of diabetic nephropathy." Bioorganic & Medicinal Chemistry Letters (2017). WB ; Rat. 222PubMed:28802633 111 [IF=1.51] Erbas et al. Oxytocin alleviates cisplatin-induced renal damage in rats. (2014) Iran.J.Basic.Med.Sci. 17:747-52 IHC ; Rat. 222PubMed:25729542 111 [IF=1.922] Chi et al. Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. (2018) Mol.Med.Rep. 17:6337-6344 IHC ; Human. 222PubMed:29512784 111 [IF=1.92] Wang et al. Extracellular matrix promotes proliferation, migration and adhesion of airway smooth muscle cells in a rat model of chronic obstructive pulmonary disease via upregulation of the PI3K/AKT signaling pathway. (2018) Mol.Med.Rep. 18:3143-3152 WB ; Rat. 222PubMed:30066869 111 [IF=2.884] Li HT et al. CpG-ODNs and Budesonide Act Synergistically to Improve Allergic Responses in Combined Allergic Rhinitis and Asthma Syndrome Induced by Chronic Exposure to Ovalbumin by Modulating the TSLP-DC-OX40L Axis.Inflammation. 2018 Aug;41(4):1304-1320. IHC ; Mouse. 222PubMed:29654433 111 [IF=5.5] Zhang et al. Cardiac Contractility Modulation Attenuate Myocardial Fibrosis by Inhibiting TGF-β1/Smad3 Signaling Pathway in a Rabbit Model of Chronic Heart Failure. (2016) Cell.Physiol.Biochem. 39:294-306 WB ; Rabbit. 222PubMed:27344462 111 [IF=7.901] Lin S et al. Strategy for hypertrophic scar therapy: Improved delivery of triamcinolone acetonide using mechanically robust tip-concentrated dissolving microneedle array. J Control Release. 2019 May 27;306:69-82. WB ; Rabbit. 222PubMed:31145948 111 [IF=3.367] Liu M et al. The effects of CRISPR-Cas9 knockout of the TGF-β1 gene on antler cartilage cells in vitro. Cell Mol Biol Lett. 2019 Jun 22;24:44. WB ; Deer. 222PubMed:31285745 111 [IF=0] Afdal A et al. The Expression of Transforming Growth Factor Beta-1 and Interleukin-6 on Human Prostate: Prostate Hyperplasia and Prostate Cancer. Open Access Maced J Med Sci. 2019 Jun 30;7(12):1905-1910. IHC-P ; Human. 222PubMed:31406527 111 [IF=3.571] Zheng HX et al. Cyanidin-3-glucoside from Black Rice Ameliorates Diabetic Nephropathy via Reducing Blood Glucose, Suppressing Oxidative Stress and Inflammation, and Regulating Transforming Growth Factor β1/Smad Expression. J Agric Food Chem. 2020 Apr 15;6 IHC-P ; Rat. 222PubMed:32192334 111 [IF=3.701] S Q et al. Cyanidin-3-glucoside from black rice prevents renal dysfunction and renal fibrosis in streptozotocin-diabetic rats. Journal of Functional Foods,(2020)72, 104062. IHC-P ; Rat. 222111 [IF=4.679] Le G et al. Ochratoxin A induces glomerular injury through activating the ERK/NF-κB signaling pathway. Food Chem Toxicol. 2020 Sep;143:111516. WB&ICF ; Human. 222PubMed:32615238 111 [IF=1.813] Li Y et al. Study on the Anticancer Effect of an Astragaloside- and Chlorogenic Acid-Containing Herbal Medicine (RLT-03) in Breast Cancer. Evid Based Complement Alternat Med. 2020 Jun 10;2020:1515081. IHC-P ; Human. 222PubMed:32595723 111 [IF=4.679] Le G et al. Ochratoxin A Induces Glomerular Injury Through Activating the ERK/NF-κB Signaling Pathway. Food Chem Toxicol. 2020 Jun 29;111516. WB,IF ; human. 222PubMed:32615238 111 [IF=4.679] Guannan Leet al. Ochratoxin A induces glomerular injury through activating the ERK/NF-κB signaling pathway. Food Chem Toxicol . 2020 Sep;143:111516. WB ; Human. 222PubMed:32615238 研究领域肿瘤 心血管 信号转导 干细胞 生长因子和激素 转录调节因子 细胞因子 细胞外基质 抗体来源Rabbit克隆类型Polyclonal交叉反应Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, )产品应用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /Test IF=1:100-500 （石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.理论分子量12.8/44kDa细胞定位分泌型蛋白 性 状Liquid浓 度1mg/ml免 疫 原KLH conjugated synthetic peptide derived from human TGF-Beta 1: 351-390/390 亚 型IgG纯化方法affinity purified by Protein A缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.PubMedPubMed 产品介绍This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016]Function:Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.Subunit:Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the'small latent TGF-beta1 complex'. Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3 (By similarity). May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition (By similarity). Interacts with CD109, DPT and ASPN.Subcellular Location:Secreted, extracellular space, extracellular matrix.Tissue Specificity:Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.Post-translational modifications:Glycosylated.The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.DISEASE:Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.Similarity:Belongs to the TGF-beta family.SWISS:P01137Gene ID:7040Database links:Entrez Gene: 7040 HumanEntrez Gene: 21803 MouseEntrez Gene: 59086 RatOmim: 190180 HumanSwissProt: P01137 HumanSwissProt: P04202 MouseSwissProt: P17246 RatUnigene: 645227 Human生长因子和激素（ Growth Factor and Hormones） TGF是一种多效生长因子，对上皮细胞增值有潜在抑制作用，可抑制肿瘤生长用于许多恶性肿瘤如:胃癌、肺癌、膀胱癌、肾癌、前列腺癌、结肠癌等多种恶性肿瘤的研究。 TGFβ超级家族由为数众多的生长的分化因子组成，包括转移生长因子β1，2和3(TGF β1，TGF β2，TGF β3)；胎盘生长因子(PTGF-β)；生长/分化因子(GDFs)；缪氏抑制物(MIS)；骨形态形成蛋白(BMPs)；交织细胞元神经生长因子(GDNF)；抑制素和活化素(α, β-A,和β-C)，Lefty和Nodal。 TGF超级家族成员参与胚胎发育和成体组织体内平衡。TGF-β1抗体与TGF-β2和TGF-β3没有交叉反应。与豚鼠有大部分交叉。产品图片Sample:Liver (Mouse) Lysate at 40 ugLiver (Rat) Lysate at 40 ugPrimary: Anti- TGF beta 1 (bs-0086R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 12.8/44 kDObserved band size: 55 kDSample:Ovary (Mouse) Lysate at 40 ugPrimary: Anti-TGF beta 1 (bs-0086R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 12.8/44 kDObserved band size: 59 kDSample:Embryo (Mouse) Lysate at 40 ugCerebrum (Rat) Lysate at 40 ugPrimary: Anti-TGF beta 1 (bs-0086R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 12.8/44 kDObserved band size: 59 kDSample:TGF beta1 recombinant protein (human) Lysate at 100ngPrimary: Anti- TGF beta1 (bs-0086R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 12.8/44 kDObserved band size: 12.8 kDSample:Lane 1: Stomach (Mouse) Lysate at 40 ugLane 2: Liver (Mouse) Lysate at 40 ugLane 3: Placenta (Mouse) Lysate at 40 ugLane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ugLane 5: Liver (Rat) Lysate at 40 ugPrimary: Anti-TGF beta 1 (bs-0086R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50/25/12.5 kDObserved band size: 50 kDParaformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TGF beta 1) Polyclonal Antibody, Unconjugated (bs-0086R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Blank control:MG63.Primary Antibody (green line): Rabbit Anti-TGF beta 1 antibody (bs-0086R)Dilution: 1μg /10^6 cells;Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 1μg /test.ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
赛国生物为您提供69个 Bioss/博奥森 ，如在选择 Bioss/博奥森产品 有疑问可以联系在线客服。
