鲑鱼精DNA 10mg/ml产品简介别名单链DNA储存条件-20℃,有效期1年规格1ml单位支Solarbio生产的鲑鱼精DNA溶液(10mg/mL)是经过酚氯仿抽提,超声和热变性处理的短片段的单链DNA溶液,可直接用于Southern、Northern等核酸杂交中。 本产品鲑鱼精DNA的浓度为10mg/mL,使用时按实验具体要求操作,稀释至所需工作浓度即可。 鲑鱼精DNA注意事项: 1. 如果每次的使用量很小,可以适当分装后再使用,避免反复冻融。 2. 为了您的安全和健康,请穿实验服并戴手套操作。
伊文斯蓝染色液(0.5%)产品简介CAS314-13-6英文名称Evan's Blue Stain Solution, 0.5%别名;伊文思兰;偶氮蓝;依文氏兰;依文思蓝;依文斯兰;埃文斯蓝;6,6'-[[3,3'-二甲基(1,1'-二苯基)-4,4'-二基]双(偶氮基)]双(4-氨基-5-羟基-1,3-萘二磺酸)四钠盐分子式C34H24N6Na4O14S4分子量960.81储存条件室温,避光保存,有效期1年规格100mL 500mL单位瓶伊文斯兰(Evans Blue)又称偶氮蓝。伊文斯兰与台盼蓝都是细胞活性染料,常用于检测细胞膜的完整性和细胞是否存活。活细胞因有外排功能而无法被伊文斯兰染成蓝色,而死细胞会被染成淡蓝色。因此可以通过此方法在显微镜下区分死细胞与活细胞,但无法区分死亡与坏死。备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.
赤霉素(GA3)产品简介英文名称Gibberellic acidCAS77-06-5级别BR别名赤霉酸;赤霉素A;2,4a,7-三羟基-1-甲基-8-亚甲基赤霉-3-烯-1,10-二羧酸-1,4a-内酯;九二零纯度90.0%分子式C19H22O6分子量346.37外观(性状)白色粉末储存条件RT有效期2年溶解性50mg/ml EtOH规格5g单位瓶赤霉素是植物生长激素,能促进茎、叶的生长,提早抽苔开花,促进种子、块茎、块根发芽,刺激果实生长,增加结果率或形成无籽果实等.结马铃薯、蕃茄、稻、麦、棉花、大豆、豌豆、烟草、果树等均有增产作用。但施用过多的赤霉素,植物会出现黄而细长的枝条,即失绿、徒长现象,反而影响产量。赤霉素还可用于从大麦制造麦芽。它对昆虫的发育也有促进作用。能溶于醇、酮、酯类等有机溶剂和pH值6.3的磷酸缓冲溶液,难溶于醚、氯仿、苯和水。与钾、钠离子形成盐并溶于水。赤霉素固体比较稳定,在干燥、密闭条件下贮存时间较久不会失效;水溶液不稳定,低温时短期贮存不致失效,温度较高时分解较快;酸性或弱酸性条件较稳定,碱性条件不稳定。 备注:产品信息可能会有优化升级。请以实际标签信息为准。 备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.
维生素C产品简介CAS50-81-7中文名称维生素C英文名称L-Ascorbic Acid别名L(+)-Ascorbic acid;Vitamin C;L-抗坏血酸纯度HPLC≥98%分子式C6H8O6 分子量176.12外观(性状)White to off-white Solid储存条件Powder : 2-8℃, 2 years; In solvent(母液): -20℃, 1 month; -80℃, 6 months溶解性Soluble in Water ≥20mg/mLMDLMFCD00064328ECEINECS 200-066-2InChIKeyCIWBSHSKHKDKBQ-JLAZNSOCSA-NInChIInChI=1S/C6H8O6/c7-1-2(8)5-3(9)4(10)6(11)12-5/h2,5,7-10H,1H2/t2-,5+/m0/s1PubChem CID54670067SMILESOC([C@@]1([H])[C@H](CO)O)=C(O)C(O1)=O描述L-Ascorbic Acid是一种有效的还原剂和抗氧化剂。(L-Ascorbic Acid is a potent reducing agent and antioxidant.)生物活性L-Ascorbic acid exhibits anti-cancer effects through the generation of reactive oxygen species (ROS) and selective damage to cancer cells[1].In VitroThe anti-cancer effects of L-Ascorbic acid are determined by sodium-dependent vitamin C transporter 2 (SVCT-2), a transporter of L-ascorbic acid. L-Ascorbic acid (0.1 μM-2 mM) exhibits anti-cancer effects according to SVCT-2 expression and L-ascorbic acid uptake. Human colorectal cancer cell lines displays differential responses to L-ascorbic acid, primarily depending on the expression level of SVCT-2[1].In VivoL-Ascorbic acid/Tolbutamide produces hypoglycaemic activity in a dose dependant manner in normal (60 mg/kg) and diabetic (40 mg/kg) condition. In the presence of L-ascorbic acid, Tolbuatmide (20 mg/kg) produces early onset of action and maintained for longer period compared to Tolbutamide matching control[2].数据来源文献[1]. Sungrae Cho, et al. Hormetic dose response to L-ascorbic acid as an anti-cancer drug in colorectal cancer cell lines according to SVCT-2 expression. Sci Rep. 2018 Jul 27;8(1):11372.[2]. Satyanarayana Sreemantula, et al. Influence of antioxidant (L- ascorbic acid) on tolbutamide induced hypoglycaemia/antihyperglycaemia in normal and diabetic rats. BMC Endocr Disord. 2005 Mar 3;5(1):2.规格20mg 10mM*1mL (in Water) 100mg单位瓶一种重要的营养素和氧化还原剂,在骨盐代谢及骨形成中具有重要作用。在体外,它能增加钙盐的沉积,促进矿化结节的形成,并通过间充质细胞间接调节破骨细胞的分化。维生素C能直接抑制核因子κB受体活化子配体(RANKL)诱导的破骨细胞形成 。 使用本产品的应用案例(仅供参考) In VitroCell(Osteogenic/成骨诱导;50 mg/l ascorbic acid) The osteogenic medium was the complete culture medium supplemented with 10 nM dexamethasone(Solarbio), 10 mM β-glycerophosphate (Solarbio), and 50 mg/l ascorbic acid (Solarbio). 数据来源文献:Jia L, Zhang Y, Ji Y, Li X, Xing Y, Wen Y, Huang H, Xu X. Comparative analysis of lncRNA and mRNA expression profiles between periodontal ligament stem cells and gingival mesenchymal stem cells. Gene. 2019 May 30;699:155-164. doi: 10.1016/j.gene.2019.03.015. Epub 2019 Mar 12. PMID: 30876821. Cell(Osteogenic/成骨诱导;50 mg/l ascorbic acid) Osteogenic induced medium contained complete culture medium supplemented with 100 nM dexamethasone (Solarbio, Beijing, China), 10 mM β-glycerophosphate (Solarbio) and 50 mg/l ascorbic acid (Solarbio). The medium was replaced every other day. Alizarin Red–positive mineralized matrix was used to evaluate osteogenic capacity by Alizarin red (AR) staining. 数据来源文献:Zhao Y, Liu H, Xi X, Chen S, Liu D. TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT. Exp Cell Res. 2020 Dec 1;397(1):112336. doi: 10.1016/j.yexcr.2020.112336. Epub 2020 Oct 19. PMID: 33091421. In Vivo Rat(雄性新生SD Rat;100 mg vitamin C/kg bw/d;灌胃;30d) Forty-five male neonatal SD rats were randomly divided into five groups (n=9 each): corn oil control group, 500 mg DEHP/kg of body weight per day (bw/d) group, 500 mg DEHP/kg bw/d+200 mg vitamin E/kg bw/d group, 500 mg DEHP/kg bw/d+100 mg vitamin C/kg bw/d group, and 500 mg DEHP/kg bw/d+200 mg vitamin E/kg bw/d+100 mg vitamin C/kg bw/d group. DEHP and vitamin E (Solarbio, China) were dissolved in corn oil, and vitamin C (Solarbio,China) was dissolved in distilled water. From postnatal day (PND) 1 to 30, the male rats were administered corn oil, DEHP, vitamin C, and/or vitamin E by gavage each day according to their group. On PND 36, six rats of each groups were sacrificed and their testes were immediately isolated. The rest of rats were fed with routine feed until PND 90. 来源文献:Tang X, Wu S, Shen L, Wei Y, Cao X, Wang Y, Long C, Zhou Y, Li D, Huang F, Liu B, Wei G. Di-(2-ethylhexyl) phthalate (DEHP)-induced testicular toxicity through Nrf2-mediated Notch1 signaling pathway in Sprague-Dawley rats. Environ Toxicol. 2018 Jul;33(7):720-728. doi: 10.1002/tox.22559. Epub 2018 Apr 16. PMID: 29663635. 备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods
PerCP/Cyanine5.5 Anti-Human CD3 Antibody[OKT-3]Uniprot : P07766 货号: E-AB-F1001J 应用: FCM Isotype: Mouse IgG2a, κ 宿主: Mouse Antigen Details Alternate NamesT-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3ESwissprotP07766 References1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.3. Lanier L, et al. 1986. J. Immunol. 137:2501.Product Details FormLiquidClone No.OKT-3 (See Other Available Formats) HostMouseIsotypeMouse IgG2a, κIsotype ControlPerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802J] ReactivityHumanApplicationFCM (See the Application Description) Storage BufferPhosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.Recommended UseEach lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.ShippingBiological ice pack at 4℃Stability & StorageKeep as concentrated solution.Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.Centrifuge before opening to ensure complete recovery of vial contents.This product is guaranteed up to one year from purchase.ConjugationPerCP/Cyanine5.5PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).如何通过光谱图选择合适的检测通道? Background CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
猪血清(无菌过滤)货号:S9060 保存:-20°C 有效期:5 年 产品说明: 本产品采用猪血液为原料,无菌采集、批量混合,经 0.1μm 微孔滤芯无菌过滤分装而成。 本品未经过灭活,溶液状态下为黄色澄清液体,无溶血或异物,长时间静置会有少量蛋白析出,使用前请震荡混匀。 产品应用(仅供参考): 本品适合用于普通细胞培养,也可用于配制试剂用。 使用时需要注意以下几点: 1. 血清一般储存于-20℃,使用时为避免反复冻融,建议无菌环境下分装成小包装,储存于-20℃。 2. 血清融化时最好先置于 2-8℃冰箱中使之溶解,然后在室温下使之全溶,溶解过程中必须规则地摇晃均匀。 3. 融化后的血清在 4℃不宜长时间存放,应尽快使用 注意事项: 1. 产品信息仅供参考,如有疑问请致电 400-968-6088 咨询。 2. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。 3. 为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
转铁蛋白产品简介英文名称 Transferrin CAS 11096-37-0 纯度 min.98% 外观(性状) 白色至浅黄色固体 储存条件 2-8℃ 有效期 2年 溶解性 10 mg/mL in water 规格 10mg 单位 支 产品说明: 转铁蛋白又名运铁蛋白 transferrin,TRF,siderophilin)是血浆中主要的含铁蛋白质,负责运载由消化管吸收的铁和由红细胞降解释放的铁。以 TRF-Fe3+的复合物形式进入骨髓中,供成熟红细胞的生成。 转铁蛋白是一种能传递铁离子的蛋白质。用于生化研究。 别名:铁传递蛋白 铁蛋白 人转铁蛋白 纯度:98% 溶解性:10 mg/mL in water 注意事项: 1. 我司生产的生化试剂如无特殊标注,基本为非无菌包装,若用于细胞实验,请提前做好预处理。 2. 一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 3. 产品信息仅供参考,如有疑问请致电 400-968-6088 咨询。 4. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。 5. 为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
总抗氧化能力(T-AOC)检测试剂盒(FRAP法)产品简介储存条件2-8℃有效期6个月中文名称总抗氧化能力(T-AOC)检测试剂盒(FRAP法)英文名称Total Antioxidant Capacity(T-AOC) Assay Kit(FRAP Method)检测方法可见分光光度法可见分光光度法 Spectrophotometer单位盒规格50T/48S测定意义:测定对象中各种抗氧化物质和抗氧化酶等构成总抗氧化水平。在生物学、医学和药学研究中常常检测血浆、血清、唾液、尿液等各种体液,细胞或组织等裂解液、植物或中草药抽提液及各种抗氧化物(antioxidant)溶液的总抗氧化能力。 测定原理:在酸性环境下,物质还原Fe3+-三吡啶三吖嗪(Fe3+-TPTZ)产生蓝色的Fe2+-TPTZ的能力反映了其总抗氧化能力。 自备实验用品:可见分光光度计、恒温水浴锅、低温离心机、1mL玻璃比色皿和蒸馏水。 相关论文:A C2H2 zinc-finger protein OsZFP213 interacts with OsMAPK3 to enhance salt tolerance in rice - PubMed (nih.gov)
中性树胶产品简介英文名称Neutral balsamCAS96949-21-2级别FMP grade别名中性树脂外观(性状)无色至淡黄色液体有效期2年储存条件RT规格100ml单位瓶显微技术上使用的优良封藏剂,与加拿大树胶性质相似,用途相同,效果较好。中性树胶与加拿大树胶相同,是一种天然树脂,经提炼与中和后溶解在透明剂中成60%溶液,折光率与玻璃近似,干燥后凝结成透明无色的固体,没有收缩变黄、纹裂的弊病,没有像加拿大树胶有年久变黄切片氧化退色等缺点。切片标本有苏木精、曙红、番红、固绿、洋红染色的,保藏数年颜色无变化。许多整装标本如吸血虫、涤虫等寄生虫,用中性树胶封固,均能取得优良效果。目前中性树胶广泛使用在生物学,医学作为生物切片封藏剂,以及光学琉璃仪器的粘固剂。使用说明:封片方法很简单,在载玻片中央滴2-3滴树胶液(注意要滴在一起否则容易有气泡),然后把盖玻片慢慢放上去,轻轻挤压,树胶液会向外侧扩散至整个镜片,通风晾几个小时后用棉球粘上透明剂或者松节油把镜片边缘多余的树胶擦洗干净。然后再放在通风处晾干,封片可常温保存。备注:产品信息可能会有优化升级。请以实际标签信息为准。 备注:以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these methods.相关产品: P1010 0.01M PBS 干粉,PH7.2-7.4 C1010 0.01M 柠檬酸钠缓冲液 pH6.0 X1020 胰蛋白酶消化液 0.1% T1080 20×TBS DA1010 DAB 显色试剂盒 PR1100 BCIP/NBT 显色试剂盒 P2100 10×多聚赖氨酸 S2100 抗荧光衰减封片剂
Human C1QTNF3(C1q and Tumor Necrosis Factor Related Protein 3) ELISA Kit文献(2) 产品货号:E-EL-H5523c 中文名:人C1q肿瘤坏死因子相关蛋白3(C1QTNF3)酶联免疫吸附测定试剂盒别名:tumor necrosis factor related protein 3, C1q and TNF related 3, Collagenous repeat-containing sequence 26 kDa protein, Secretory protein CORS26 用途 该试剂盒用于体外定量检测Human 血清,血浆或其他生物体液中C1QTNF3浓度检测原理 本试剂盒采用双抗体夹心ELISA法。用抗人C1QTNF3抗体包被于酶标板上,实验时样品或标准品中的人C1QTNF3会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗人C1QTNF3抗体和辣根过氧化物酶标记的亲和素。抗人C1QTNF3抗体与结合在包被抗体上的人C1QTNF3结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,C1QTNF3浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中C1QTNF3的浓度。反应类型 夹心法规格 96T反应时间 3.5h反应性 Human检测方法 Colormetric检测范围 0.63—40 ng/mL灵敏度 0.38 ng/mL样本体积 100μL样本类型 血清,血浆或其他生物体液特异性 可检测样本中的人C1QTNF3,且与其它相关蛋白无明显交叉反应。重复性 板内,板间变异系数均<10%。典型数据 由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。(ng/mL) O.D Average Corrected 402.3862.432.4082.334201.5631.5731.5681.494100.9070.8810.8940.8250.4770.5110.4940.422.50.2810.2670.2740.21.250.1820.160.1710.0970.630.1170.1310.1240.0500.0730.0750.074--精密度板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(ng/mL) 2.126.1713.891.966.0914.78Standarddeviation 0.110.310.510.130.270.81C V (%) 5.195.023.676.634.435.48回收率 分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 92-10698EDTA plasma (n=8) 87-9893Cell culture media (n=8) 91-10597线性 分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)88-10496-10790-101Average (%)95102951:4Range (%)90-10482-9382-95Average (%)9687881:8Range (%)86-10185-9884-99Average (%)9390901:16Range (%)94-10987-10184-95Average (%)999290试剂盒组成及保存 未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。中文名称 英文名称 规格 保存条件 ELISA酶标板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 条-20℃,可存放6个月冻干标准品Reference Standard2 支浓缩生物素化抗体(100×)Concentrated Biotinylated Detection Ab1支 120 μL浓缩HRP酶结合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光),可存放6个月标准品&样品稀释液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6个月生物素化抗体稀释液Biotinylated Detection Ab Diluent1瓶 14 mL酶结合物稀释液HRP Conjugate Diluent1瓶 14 mL浓缩洗涤液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反应终止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 张产品说明书Product Description1 份质检报告Certificate of Analysis1 份说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。试验所需自备物品 1. 酶标仪(450nm波长滤光片)2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒温箱,双蒸水或去离子水4. 吸水纸操作步骤1. 对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟2. 弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟3. 弃掉板内液体,洗板3次4. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次5. 每孔加入90μL底物溶液,37℃孵育15分钟左右6. 每孔加入50μL终止液7. 立即在450nm波长下读数,处理数据
产品货号:E-EL-M1084c中文名:小鼠肿瘤坏死因子相关凋亡诱导配体(TRAIL/TNFSF10)酶联免疫吸附测定试剂盒别称:APO2L, Apo2-L, TL2, CD253用途该试剂盒用于体外定量检测Mouse 血清,血浆或其他生物体液中TRAIL/TNFSF10浓度检测原理本试剂盒采用双抗体夹心ELISA法。用抗小鼠TRAIL/TNFSF10抗体包被于酶标板上,实验时样品或标准品中的小鼠TRAIL/TNFSF10会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗小鼠TRAIL/TNFSF10抗体和辣根过氧化物酶标记的亲和素。抗小鼠TRAIL/TNFSF10抗体与结合在包被抗体上的小鼠TRAIL/TNFSF10结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,TRAIL/TNFSF10浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中TRAIL/TNFSF10的浓度。反应类型夹心法规格96T反应时间3.5h反应性Mouse检测方法Colormetric检测范围15.63—1000 pg/mL灵敏度9.38 pg/mL样本体积100μL样本类型血清,血浆或其他生物体液特异性可检测样本中的小鼠TRAIL/TNFSF10,且与其它相关蛋白无明显交叉反应。重复性板内,板间变异系数均<10%。典型数据由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。(pg/mL)O.DAverageCorrected10002.4692.5172.4932.4255001.5871.5971.5921.5242500.970.9410.9560.8881250.4370.4650.4510.38362.50.240.2380.2390.17131.250.1720.1680.170.10215.630.110.130.120.05200.0660.0700.068--精密度板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。Intra-assay PrecisionInter-assay PrecisionSample123123n202020202020Mean(pg/mL)50.22124.92346.8749.84136.70361.81Standarddeviation2.695.8011.342.825.7813.28C V (%)5.364.643.275.664.233.67回收率分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。Sample TypeRange (%)Average Recovery (%)Serum (n=8)91-10597EDTA plasma (n=8)93-10799Cell culture media (n=8)92-10599线性分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)1:2Range (%)87-10185-9996-110Average (%)92921011:4Range (%)91-10186-10087-100Average (%)9692921:8Range (%)86-9982-9388-101Average (%)9388931:16Range (%)86-10085-9786-101Average (%)939093试剂盒组成及保存未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。中文名称英文名称规格保存条件ELISA酶标板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 条-20℃,可存放6个月冻干标准品Reference Standard2 支浓缩生物素化抗体(100×)Concentrated Biotinylated Detection Ab1支 120 μL浓缩HRP酶结合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光),可存放6个月标准品&样品稀释液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6个月生物素化抗体稀释液Biotinylated Detection Ab Diluent1瓶 14 mL酶结合物稀释液HRP Conjugate Diluent1瓶 14 mL浓缩洗涤液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反应终止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 张产品说明书Product Description1 份质检报告Certificate of Analysis1 份说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。试验所需自备物品1. 酶标仪(450nm波长滤光片)2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒温箱,双蒸水或去离子水4. 吸水纸操作步骤1. 对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟2. 弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟3. 弃掉板内液体,洗板3次4. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次5. 每孔加入90μL底物溶液,37℃孵育15分钟左右6. 每孔加入50μL终止液7. 立即在450nm波长下读数,处理数据客户发表文献Pseudolaric acid B ameliorates synovial inflammation and vessel formation by stabilizing PPARγ to inhibit NF-κB signalling pathwayIF: 5.31Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE(2021)DOI: 10.1111/jcmm.16670PMID: 34117708样本类型: Raw 264.7 cells物种: Mouse产品货号:E-EL-M1084c中文名:小鼠肿瘤坏死因子相关凋亡诱导配体(TRAIL/TNFSF10)酶联免疫吸附测定试剂盒别称:APO2L, Apo2-L, TL2, CD253
Rat IL-11(Interleukin 11) ELISA Kit产品货号:E-EL-R0562c 中文名:大鼠白介素11(IL-11)酶联免疫吸附测定试剂盒别称:IL11, AGIF, IL-11, interleukin 11用途 该试剂盒用于体外定量检测Rat 血清,血浆或其他生物体液中IL-11浓度检测原理 本试剂盒采用双抗体夹心ELISA法。用抗大鼠IL-11抗体包被于酶标板上,实验时样品或标准品中的大鼠IL-11会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗大鼠IL-11抗体和辣根过氧化物酶标记的亲和素。抗大鼠IL-11抗体与结合在包被抗体上的大鼠IL-11结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IL-11浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-11的浓度。反应类型 夹心法规格 96T反应时间 3.5h反应性 Rat检测方法 Colormetric检测范围 15.63—1000 pg/mL灵敏度 9.38 pg/mL样本体积 100μL样本类型 血清,血浆或其他生物体液特异性 可检测样本中的大鼠IL-11,且与其它相关蛋白无明显交叉反应。重复性 板内,板间变异系数均<10%。典型数据 由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。(pg/mL) O.D Average Corrected 10002.4832.5052.4942.4195001.6431.6991.6711.5962500.990.9660.9780.9031250.450.4760.4630.38862.50.2740.2520.2630.18831.250.1820.170.1760.10115.630.1180.1360.1270.05200.0730.0770.075--精密度板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 47.98127.48499.3948.54135.62511.36Standarddeviation 3.056.9720.282.997.5119.12C V (%) 6.365.474.066.165.543.74回收率 分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 94-109101EDTA plasma (n=8) 96-112102Cell culture media (n=8) 90-10195线性 分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)93-10795-10996-110Average (%)981021031:4Range (%)91-10979-9285-97Average (%)9985901:8Range (%)89-10280-9387-100Average (%)9686921:16Range (%)93-10679-9387-99Average (%)1008694试剂盒组成及保存 未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。中文名称 英文名称 规格 保存条件 ELISA酶标板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 条-20℃,可存放6个月冻干标准品Reference Standard2 支浓缩生物素化抗体(100×)Concentrated Biotinylated Detection Ab1支 120 μL浓缩HRP酶结合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光),可存放6个月标准品&样品稀释液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6个月生物素化抗体稀释液Biotinylated Detection Ab Diluent1瓶 14 mL酶结合物稀释液HRP Conjugate Diluent1瓶 14 mL浓缩洗涤液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反应终止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 张产品说明书Product Description1 份质检报告Certificate of Analysis1 份说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。试验所需自备物品 1. 酶标仪(450nm波长滤光片)2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒温箱,双蒸水或去离子水4. 吸水纸操作步骤1. 对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟2. 弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟3. 弃掉板内液体,洗板3次4. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次5. 每孔加入90μL底物溶液,37℃孵育15分钟左右6. 每孔加入50μL终止液7. 立即在450nm波长下读数,处理数据
Mouse IL-11(Interleukin 11) ELISA Kit文献(1) 产品货号:E-EL-M0725c 中文名:小鼠白介素11(IL-11)酶联免疫吸附测定试剂盒别称:IL11, AGIF, IL-11, interleukin 11用途 该试剂盒用于体外定量检测Mouse 血清,血浆或其他生物体液中IL-11浓度检测原理 本试剂盒采用双抗体夹心ELISA法。用抗小鼠IL-11抗体包被于酶标板上,实验时样品或标准品中的小鼠IL-11会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗小鼠IL-11抗体和辣根过氧化物酶标记的亲和素。抗小鼠IL-11抗体与结合在包被抗体上的小鼠IL-11结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IL-11浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-11的浓度。反应类型 夹心法规格 96T反应时间 3.5h反应性 Mouse检测方法 Colormetric检测范围 15.63—1000 pg/mL灵敏度 9.38 pg/mL样本体积 100μL样本类型 血清,血浆或其他生物体液特异性 可检测样本中的小鼠IL-11,且与其它相关蛋白无明显交叉反应。重复性 板内,板间变异系数均<10%。典型数据 由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等),标准曲线的OD值会有所差异。以下数据和曲线仅供参考,实验者需根据自己的实验建立标准曲线。(pg/mL) O.D Average Corrected 10002.4462.4522.4492.3685001.6591.7091.6841.6032500.9370.9260.9320.8511250.4770.4890.4830.40262.50.2540.240.2470.16631.250.1950.1690.1820.10115.630.1260.140.1330.05200.0730.0890.081--精密度板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 46.21133.72482.9743.29121.11460.50Standarddeviation 3.187.8517.192.945.8422.84C V (%) 6.885.873.566.794.824.96回收率 分别往5个不同样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 86-9991EDTA plasma (n=8) 96-106101Cell culture media (n=8) 96-108101线性 分别往5个样本中添加已知浓度的目标蛋白,做回收实验,得出回收率范围及平均回收率。将5个样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)85-9684-9587-99Average (%)9190941:4Range (%)91-10585-9887-97Average (%)9691921:8Range (%)92-10383-9683-97Average (%)9888901:16Range (%)91-10587-10086-99Average (%)979291试剂盒组成及保存 未拆封的试剂盒可在2-8℃保存一周;如果一周以后才使用试剂盒,请拆开试剂盒按照下表中的条件分别保存各组分。中文名称 英文名称 规格 保存条件 ELISA酶标板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 条-20℃,可存放6个月冻干标准品Reference Standard2 支浓缩生物素化抗体(100×)Concentrated Biotinylated Detection Ab1支 120 μL浓缩HRP酶结合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光),可存放6个月标准品&样品稀释液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6个月生物素化抗体稀释液Biotinylated Detection Ab Diluent1瓶 14 mL酶结合物稀释液HRP Conjugate Diluent1瓶 14 mL浓缩洗涤液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反应终止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 张产品说明书Product Description1 份质检报告Certificate of Analysis1 份说明:所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些,请在使用时量取而非直接倒出。试验所需自备物品 1. 酶标仪(450nm波长滤光片)2. 高精度移液器,EP管及一次性吸头:0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒温箱,双蒸水或去离子水4. 吸水纸操作步骤1. 对应板孔中加入100μL标准品工作液或样本,37℃孵育90分钟2. 弃掉板内液体后,立即加入100μL生物素化抗体工作液,37℃孵育60分钟3. 弃掉板内液体,洗板3次4. 每孔加入100μL HRP酶结合物工作液,37℃孵育30分钟,弃掉板内液体,洗板5次5. 每孔加入90μL底物溶液,37℃孵育15分钟左右6. 每孔加入50μL终止液7. 立即在450nm波长下读数,处理数据客户发表文献Baicalein alleviates fibrosis and inflammation in systemic sclerosis by regulating B-cell abnormalitiesIF: 2.838Journal: BMC Complementary Medicine and Therapies(2023)DOI: 10.1186/s12906-023-03885-1样本类型: Serum物种: Mouse
Recombinant human IL-6 protein, C-His产品编号bs-41177P英文名称Recombinant human IL-6 protein, C-His中文名称重组人白介素6蛋白别 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6. ;Recombinant human IL-6 protein, C-His Specific References (1) | bs-41177P has been referenced in 1 publications.[IF=4.966] 222Xiaolong Zhang. et al. Baicalein inhibits non‐small‐cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment. Cancer Sci. 2020 Oct; 111(10): 3802–3812 other ; PubMed:32691974理论分子量15kDa检测分子量21 kDa性 状Lyophilized or Liquid浓 度>0.5 mg/ml物 种Human序 列30-212/212纯 度>95% as determined by SDS-PAGE纯化方法AC内毒素Not analyzed表达系统E.coli活性Not tested标签C-His缓 冲 液20 mM Tris-HCl (pH=8.0) with 150 mM NaCl保存条件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacterium Streptococcus pneumoniae.SWISS:P05231Gene ID:3569产品图片
Recombinant mouse IL-6 protein, C-His产品编号bs-10861P英文名称Recombinant mouse IL-6 protein, C-His中文名称重组小鼠白介素IL-6蛋白别 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6; Recombinant mouse IL-6 protein, C-His 理论分子量23kDa检测分子量26 kDa性 状Lyophilized or Liquid浓 度>0.5 mg/ml物 种Mouse序 列25-211/211纯 度>95% as determined by SDS-PAGE纯化方法AC内毒素Not analyzed表达系统E.coli活性Not tested标签C-His缓 冲 液20 mM Tris-HCl with 20% Glycerol保存条件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011].SWISS:P08505Gene ID:16193产品图片
Recombinant human IL-22 protein产品编号bs-43572P英文名称Recombinant human IL-22 protein中文名称重组人白介素22蛋白性 状Lyophilized or Liquid浓 度1mg/ml保存条件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍产品图片
Recombinant human IL-10 protein (Active, HEK293) 产品编号ck0006英文名称Recombinant human IL-10 protein (Active, HEK293)中文名称重组人白介素10 (活性的, Tag free)别 名Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. ;Recombinant human IL-10 protein (Active, HEK293) 人白介素10 重组蛋白; 人白细胞介素10 ; 重组人白介素10; 重组人IL-10;理论分子量17kDa检测分子量21 kDa性 状Lyophilized powder浓 度>0.5 mg/ml物 种Human序 列19-178/178纯 度>95% as determined by SDS-PAGE纯化方法AC内毒素<0.5 EU/μg as determined by LAL.表达系统HEK293 cell活性Yes标签Tag free缓 冲 液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存条件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品介绍Interleukin 10(IL-10) is a member of the IL-10 family of α-helical cytokines. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of a number of proinflammatory cytokines such as IFNgamma, IL-2, IL-3, TNF and GM-CSF. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.This recombinant human IL-10 is produced by human cells.(Tag free)Biological activity:The activity was measured by its ability to stimulate the proliferation of MC/9-2 cells (mouse master cell line).Reconstitution:Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.SWISS:P18893Gene ID:3586产品图片
产品编号V0401英文名称human IL-6中文名称人白介素6单克隆抗体别 名Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; Interleukin-6; Interleukin6; IL6_HUMAN. 研究领域肿瘤 细胞生物 免疫学 神经生物学 生长因子和激素 抗体来源Mouse克隆类型Monoclonal克 隆 号6F5K交叉反应(predicted: Human, )产品应用ELISA=1:5000-10000 not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.理论分子量23kDa细胞定位分泌型蛋白 性 状Liquid浓 度≥1mg/ml免 疫 原Recombinant human IL-6 (C-6x His-Tag) 亚 型IgG1纯化方法affinity purified by Protein G缓 冲 液0.01M PBS(pH7.4) with 0.1% Proclin300.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.PubMedPubMed 产品介绍Interleukin 6 (IL-6), promptly and transiently produced in response to infections and tissue injuries, contributes to host defense through the stimulation of acute phase responses, hematopoiesis, and immune reactions.Function:Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.Subcellular Location:Secreted.Post-translational modifications:N- and O-glycosylated.DISEASE:Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.Similarity:Belongs to the IL-6 superfamily.SWISS:P05231Gene ID:3569Database links:Entrez Gene: 3569 HumanEntrez Gene: 16193 MouseEntrez Gene: 24498 RatOmim: 147620 HumanSwissProt: P05231 HumanSwissProt: P08505 MouseSwissProt: P20607 RatUnigene: 654458 HumanUnigene: 1019 MouseUnigene: 9873 Rat