G49479 ，Guanidine thiocyanate，异硫氰酸胍

625.00元

产品特性pH值5 - 7特色行业Agriculture灰分<=0.1 %级别for molecular biology痕量阳离子Fe: <=1 ppm熔点118 - 121 °C(lit.)含量>=99.5%杂质<=0.3% water外源活性位点DNase, RNase, none detected储存温度常温应用离液剂和强变性剂；溶解细胞。

G40587 ,Guanidine hydrochloride ,盐酸胍

952.00元

产品特性可溶性H2O: soluble6 M, clear (100 Hazen)熔点180-185°C(lit.)含量>=99.5%应用强离液剂，可用于蛋白质的变性和随后的复性。 这种强变性剂可以溶解不溶性或变性的蛋白质，例如包涵体。 这可以用作将蛋白质或酶重折叠成其活性形式的第一步。 尿素和二硫苏糖醇（DTT）也可能是必需的。

G40587 , Guanidine hydrochloride ,盐酸胍

176.00元

产品特性可溶性H2O: soluble6 M, clear (100 Hazen)熔点180-185°C(lit.)含量>=99.5%应用强离液剂，可用于蛋白质的变性和随后的复性。 这种强变性剂可以溶解不溶性或变性的蛋白质，例如包涵体。 这可以用作将蛋白质或酶重折叠成其活性形式的第一步。 尿素和二硫苏糖醇（DTT）也可能是必需的。

P15883-1L,TWEEN 80 | 吐温80

495.00元

产品特性HLB15转变温度cloud point 65°C摩尔质量average mol wt 1310摩尔质量micellar avg mol wt 79,000CMC0.012 mM(20-25C)形态viscous liquid组成Oleic acid, >=58.0% (balance primarily linoleic, palmitic, and stearic acids)描述non-ionic聚集数60应用非离子型洗涤剂，用于从哺乳动物细胞系的细胞核中选择性提取和分离蛋白质。

P15883-500ML, TWEEN 80 | 吐温80

263.00元

产品特性HLB15转变温度cloud point 65°C摩尔质量average mol wt 1310摩尔质量micellar avg mol wt 79,000CMC0.012 mM(20-25C)形态viscous liquid组成Oleic acid, >=58.0% (balance primarily linoleic, palmitic, and stearic acids)描述non-ionic聚集数60应用非离子型洗涤剂，用于从哺乳动物细胞系的细胞核中选择性提取和分离蛋白质。

P15883 , TWEEN 80 | 吐温80

173.00元

产品特性HLB15转变温度cloud point 65°C摩尔质量average mol wt 1310摩尔质量micellar avg mol wt 79,000CMC0.012 mM(20-25C)形态viscous liquid组成Oleic acid, >=58.0% (balance primarily linoleic, palmitic, and stearic acids)描述non-ionic聚集数60应用非离子型洗涤剂，用于从哺乳动物细胞系的细胞核中选择性提取和分离蛋白质。

genefist，GF1812，10X SDS Tris-Gly 电泳缓冲液

115.00元

genefist，GF0100，琼脂糖

200.00元

genefist，GF0100，琼脂糖，100g

genefist，GF1811，5X SDS-PAGE蛋白上样缓冲液

250.00元

产品简介：SDS-PAGE 上样缓冲液(SDS-PAGE Loading Buffer, 5X)，是一种经过改良的以溴酚蓝为染料，5 倍浓缩的蛋白上样缓冲液，用于 SDS-PAGE 时蛋白样品的上样操作。不含传统的 β-巯基乙醇或 DTT，Genefist 上样缓冲液采用 TCEP 作为还原剂，稳定性更好、还原效果更优，并且无难闻气味！保存条件：-20℃保存，至少一年有效。使用说明：1、在室温或不超过 37℃的水浴中溶解蛋白上样缓冲液。水浴溶解后立即室温存放，尽量避免长时间置于水浴中。使用完毕后置于-20℃保存。2、按照每 4ul 蛋白样品加入 1ul 上样缓冲液的比例，混合蛋白样品和上样缓冲液。3、100℃或沸水浴加热 5-10 分钟，以充分变性蛋白。4、冷却到室温后，上样到 SDS-PAGE 胶加样孔内，开始电泳。注意事项： 由于含有变性剂，本品不适用于非变性胶的电泳 SDS-PAGE 蛋白上样缓冲液(5X)必须完全溶解后再使用。 如果起始时细胞或组织的用量较大，基因组 DNA 含量较高，“混合液”可适当延长加热时间。 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗。

genefist，GF1600-5，通用型抗体稀释液

789.00元

产品简介通用型抗体稀释液是一种即用型的，适合于稀释所有免疫抗体测定（Immunofluorescence, IHC, ELISA, WB）实验中的抗体。该试剂可用于一抗或二抗的稀释和配制，在 4℃保存和使用不少于六个月。稀释液中加入了多种抗体结合反应增强剂及稳定剂，能够增加抗体的溶解度和稳定性，增强免疫反应，降低非特异的背景颜色，提高实验的特异性和灵敏度，可反复使用，节约宝贵的抗体。试剂中不含有动物源的蛋白，适合于所有类型抗体稀释，包括 HRP，AP 标记的抗体。不含磷酸盐、叠氮钠或硫柳汞类防腐剂，安全性更高，。产品特色 即用型稀释液，无需配制 稀释后的抗体可以使用长达 6 个月 适用所有实验的抗体稀释（IF、IHC、ELISA、WB） 含抗体结合反应增强剂、稳定剂，效果更佳 不含 BSA、脱脂奶粉等动物源蛋白 不含磷酸盐、叠氮钠或硫柳汞类防腐剂运输和保存条件常温运输，4℃存储，有效期两年。操作步骤 ： 根据抗体使用说明书，直接用本品对一抗或二抗进行适当倍数的稀释。 保存在抗体稀释液中的抗体可回收于 4℃保存，可反复使用多次，长达 6 个月以上，直至不能达到理想结果后丢弃。注意事项1、使用后请旋紧瓶盖，防止溶液挥发或与空气的物质发生化学反应，并放回 4℃保存。2、在进行抗体免疫反应前可以使用 Genefist 的高效 Western 封闭液（货号GF1815）进行封闭，能进一步提高条带的清晰度，减少显色后的高背景和杂带的产生 。3、本品不含叠氮钠或硫柳汞类防腐剂，但还需小心操作避免皮肤直接接触。4、本试剂只能用于科研实验，请勿用于临床诊断和其它用途。

genefist，GF1600-1，通用型抗体稀释液

189.00元

产品简介通用型抗体稀释液是一种即用型的，适合于稀释所有免疫抗体测定（Immunofluorescence, IHC, ELISA, WB）实验中的抗体。该试剂可用于一抗或二抗的稀释和配制，在 4℃保存和使用不少于六个月。稀释液中加入了多种抗体结合反应增强剂及稳定剂，能够增加抗体的溶解度和稳定性，增强免疫反应，降低非特异的背景颜色，提高实验的特异性和灵敏度，可反复使用，节约宝贵的抗体。试剂中不含有动物源的蛋白，适合于所有类型抗体稀释，包括 HRP，AP 标记的抗体。不含磷酸盐、叠氮钠或硫柳汞类防腐剂，安全性更高，。产品特色 即用型稀释液，无需配制 稀释后的抗体可以使用长达 6 个月 适用所有实验的抗体稀释（IF、IHC、ELISA、WB） 含抗体结合反应增强剂、稳定剂，效果更佳 不含 BSA、脱脂奶粉等动物源蛋白 不含磷酸盐、叠氮钠或硫柳汞类防腐剂运输和保存条件常温运输，4℃存储，有效期两年。操作步骤 ： 根据抗体使用说明书，直接用本品对一抗或二抗进行适当倍数的稀释。 保存在抗体稀释液中的抗体可回收于 4℃保存，可反复使用多次，长达 6 个月以上，直至不能达到理想结果后丢弃。注意事项1、使用后请旋紧瓶盖，防止溶液挥发或与空气的物质发生化学反应，并放回 4℃保存。2、在进行抗体免疫反应前可以使用 Genefist 的高效 Western 封闭液（货号GF1815）进行封闭，能进一步提高条带的清晰度，减少显色后的高背景和杂带的产生 。3、本品不含叠氮钠或硫柳汞类防腐剂，但还需小心操作避免皮肤直接接触。4、本试剂只能用于科研实验，请勿用于临床诊断和其它用途。

genefist，GF1817，5X半干转快速转膜液

318.00元

半干转膜系统快速转膜液（5X）Cat #. GF1816 / Size. 500 ml产品简介半干转膜系统快速转膜液 Western blot Rapid Transfer Buffer for semi-dry（5X）是 Genefist推出的一种 5 倍浓度的高效转膜液，能高效快速地将蛋白转移到印迹膜（PVDF 或硝酸纤维素膜）上。本转膜液使用过程中，无需再使用甲醇或乙醇等任何醇类试剂，且能在 5-15 分钟内完成转膜过程，快速、高效、安全！产品特色： 高效安全：不使用甲醇或乙醇等任何醇类试剂，减少有毒试剂使用； ˗ 快速稳定：能在 5-15 分钟完成转膜过程，产热量小，减少蛋白损失； 兼容性好：兼容 Laemmli 胶，预制胶，FastPAGE，Bis-Tris 胶等多种凝胶。运输和保存条件：常温运输，室温存储，有效期一年。操作步骤 ：1、1X 快速转膜液的配制（100ml）：取 20ml 快速转膜液（5×）+ 80ml 去离子水；2、做好转印三明治结构，然后转移至电泳槽中，设定恒压 20V，一般时间 5-15 分钟完成蛋白转膜。备注： PVDF 膜使用前要用无水甲醇润湿 30 秒以上。 转印电流建议设定恒压 20V，1mm 厚度的凝胶，一般 5 分钟可以将 150kD 以下蛋白完全转印；大于 150kD 蛋白 10-15 分钟即可完全转印。 凝胶的浓度影响转印的效率，对于胶浓度 7.5%，5-10 分钟即可完成转印；对于胶浓度 10%,，5-15 分钟即可完成转印；对于胶浓度大于 10%，建议延长至 8-20 分钟。注意事项1、使用本试剂前请仔细阅读说明书。2、本产品只能用于科研实验，请勿用于临床诊断及其它用途。

genefist，GF6619-10，三色预染蛋白marker 10-245KD (protein marker)

2650.00元

Product Information GeneFist ™series3-color High Range Protein Marker(9-245 kDa)GF6619Size:250 µl × 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6619 GeneFist™ 3-color High Range ProteinMarker is a ready to use three-color protein standardwith 12 pre-stained proteins covering a wide range ofmolecular weights from 10 to 245 kDa in Tris-Glycinebuffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins arecovalently coupled with different chromophores foreasy identification of bands, with two referenceproteins carrying enhanced intensity correspondingto a green at 25 kDa and red at 75 kDa, respectively,as separated on SDS-PAGE (Tris-Glycine buffer).The GF6619 GeneFist™ 3-color High Range ProteinMarker is designed for monitoring protein separationduring SDS-polyacrylamide gel electrophoresis,verification of Western transfer efficiency onmembranes (PVDF, nylon, or nitrocellulose) and forapproximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6619 GeneFist™ 3-color High Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue2452352352Blue1801701703Blue1401301304Blue10093935Red7570726Blue6053537Blue4541428Blue3530309Green25222310Blue20181811Blue15141412Blue109103-color High Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6619 GeneFist™ 3-color High Range Protein Marker resolves 12 majorbands in SDS-PAGE (Tris-Glycine, MOPS, and MESbuffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist 3-color Regular Range ProteinMarker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only

genefist，GF6619-01，三色预染蛋白marker 10-245KD (protein marker)

300.00元

Product Information GeneFist ™series3-color High Range Protein Marker(9-245 kDa)GF6619Size:250 µl × 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6619 GeneFist™ 3-color High Range ProteinMarker is a ready to use three-color protein standardwith 12 pre-stained proteins covering a wide range ofmolecular weights from 10 to 245 kDa in Tris-Glycinebuffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins arecovalently coupled with different chromophores foreasy identification of bands, with two referenceproteins carrying enhanced intensity correspondingto a green at 25 kDa and red at 75 kDa, respectively,as separated on SDS-PAGE (Tris-Glycine buffer).The GF6619 GeneFist™ 3-color High Range ProteinMarker is designed for monitoring protein separationduring SDS-polyacrylamide gel electrophoresis,verification of Western transfer efficiency onmembranes (PVDF, nylon, or nitrocellulose) and forapproximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6619 GeneFist™ 3-color High Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue2452352352Blue1801701703Blue1401301304Blue10093935Red7570726Blue6053537Blue4541428Blue3530309Green25222310Blue20181811Blue15141412Blue109103-color High Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6619 GeneFist™ 3-color High Range Protein Marker resolves 12 majorbands in SDS-PAGE (Tris-Glycine, MOPS, and MESbuffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist 3-color Regular Range ProteinMarker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only

genefist，GF6616-10，三色预染蛋白marker 10-180KD (protein marker)

2350.00元

Product Information GeneFist ™ series3-color Regular Range Protein Marker(9-180 kDa)GF6616Size:250 µl x 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6616 GeneFist™ 3-color Regular RangeProtein Marker is a ready to use three-color proteinstandard with 10 pre-stained proteins covering a widerange of molecular weights from 10 to 180 kDa (9 to170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDain Bis-Tris (MES) buffer). Proteins are covalentlycoupled with a blue chromophore except for tworeference bands (one green and one red band at 25kDa and 75 kDa respectively) when separated on SDSPAGE (Tris-Glycine buffer).The GF6616 GeneFist™ 3-color Regular RangeProtein Marker is designed for monitoring proteinseparation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiencyon membranes, (PVDF, nylon, or nitrocellulose) andfor approximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6616 GeneFist™ 3-color Regular Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue1801701702Blue1401301303Blue10093934Red7570725Blue6053536Blue4541427Blue3530308Green2522239Blue15141410Blue109103-color Regular Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6616 GeneFist™3-color Regular Range Protein Marker resolves 10major bands in SDS-PAGE (Tris-Glycine, MOPS, andMES buffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist Enhanced 3-color Regular RangeProtein Marker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only

genefist，GF6616-01，三色预染蛋白marker 10-180KD (protein marker)

265.00元

Product Information GeneFist ™ series3-color Regular Range Protein Marker(9-180 kDa)GF6616Size:250 µl x 15 µl per well for 100 applicationsStorage4°C for 3 months-20°C for 24 monthsLoading Volume• 3 μl or 5 μl per loading for clear visualizationduring electrophoresis on a 15-well or 10-wellmini-gel.• 1.5~2.5 μl per well for general Western blottingtransfer.• Apply more for a thicker (> 1.5 mm) or larger gel.DescriptionThe GF6616 GeneFist™ 3-color Regular RangeProtein Marker is a ready to use three-color proteinstandard with 10 pre-stained proteins covering a widerange of molecular weights from 10 to 180 kDa (9 to170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDain Bis-Tris (MES) buffer). Proteins are covalentlycoupled with a blue chromophore except for tworeference bands (one green and one red band at 25kDa and 75 kDa respectively) when separated on SDSPAGE (Tris-Glycine buffer).The GF6616 GeneFist™ 3-color Regular RangeProtein Marker is designed for monitoring proteinseparation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiencyon membranes, (PVDF, nylon, or nitrocellulose) andfor approximating the size of proteins.The marker is supplied in gel loading buffer and isready to use. Do NOT heat, dilute, or add reducingagents before loading.ContentsApproximately 0.1~0.4 mg/ml of each protein in thebuffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)Glycerol).Guide for Molecular Weight EstimationMigration patterns and approximate MWs (kDa) ofGF6616 GeneFist™ 3-color Regular Range ProteinMarker in different electrophoresis conditions arelisted below:BandColorTRISGLYCINEBIS-TRIS(MOPS)BIS-TRIS(MES)1Blue1801701702Blue1401301303Blue10093934Red7570725Blue6053536Blue4541427Blue3530308Green2522239Blue15141410Blue109103-color Regular Range Protein MarkerNote. The apparent molecular weight (kDa) of each protein has beendetermined by calibration against an unstained protein standard;supplemental data should be considered for more accurateadjustments in different electrophoresis conditions.Quality ControlUnder suggested conditions, GF6616 GeneFist™3-color Regular Range Protein Marker resolves 10major bands in SDS-PAGE (Tris-Glycine, MOPS, andMES buffer) and after Western blotting transfer to anitrocellulose membrane.Other informationGENEFIST Technology, Inc. claims all warranties withrespect to this document, expressed or implied,including but not limited to those of merchantabilityor fitness for a particular purpose. In no event shallGENEFIST Technology, Inc. be liable, whether incontract, tort, warranty, or under any statute or anyother basis for special, incidental, indirect, punitive,multiple or consequential damages in connection withor arising from this document, including but notlimited to the use thereof.Caution: Not intended for human or animal diagnostic or therapeuticuses.Related ProductsGF6150 GeneFist All Blue Regular Range ProteinMarker, 250 µl × 2GF6160 GeneFist All Blue Regular Range PlusProtein Marker, 250 µl × 2GF6170 GeneFist All Blue Broad Range ProteinMarker, 250 µl × 2GF6240 GeneFist Pink Blue Protein Marker, 250 µl × 2GF6616 GeneFist Enhanced 3-color Regular RangeProtein Marker, 250 µl × 2GF6619 GeneFist 3-color High Range ProteinMarker, 250 µl × 2GF6261 GeneFist Enhanced 3-color High RangeProtein Marker, 250 µl × 2GF6270 GeneFist 3-color Broad Range ProteinMarker, 250 µl × 2GF6280 GeneFist 3-color Extra Range ProteinMarker, 250 µl × 2GF6500 GeneFist 3-color Pre-stained Protein Ladder,Regular Range, 250 µl × 2GF6510 GeneFist 3-color Pre-stained ProteinLadder, High Range, 250 µl × 2GF6520 GeneFist 3-color Pre-stained ProteinLadder, Broad Range, 250 µl × 2GF2100 GeneFist Protein Fluorescent Staining Dye(Red, 1,000X), 1 mlGF5110 GeneFist 50 bp DNA Ladder, 500 µlGF5220 GeneFist 100 bp DNA Ladder II, 500 µlGF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,500 µlGF5510 GeneFist Super Range DNA Ladder, (50 bp-25 kb), 500 µlGF3010 GeneFist Blue Light LED epi-illuminator,AC 100-240V, 50/60HzGF1100 GeneFist Western Marker I, 250 µlGeneFist Western Marker IFor research use only

genefist，GF1800-08，8% PAGE预混胶试剂盒

261.00元

产品信息 PAGE预混胶试剂盒 CAT.# GF1800 分离胶A (200ml) 分离胶B (200ml) 浓缩胶A (75ml) 浓缩胶B (75ml) 产品描述 Ø 此试剂盒用于制备PAGE凝胶，快速、便捷；Ø 所制备的凝胶可用于变性和非变形蛋白质电泳分析；Ø 有6%、8%、10%、12%、15%五个不同浓度可供选择；Ø 预混配方，无需计算所需溶液量，无需稀释，只需两种液体等比例混合，简化传统制胶流程，缩短制胶时间；Ø 可耐受最高260V分离电压，将传统的凝胶电泳实验从1-2小时缩短至30分钟，且条带清晰、锐利；Ø 高效的蛋白转移能力，保证Western blot 数据的高度可靠性；Ø 每盒最多可制胶100块，超高性价比；Ø 不用冷藏或冷冻存贮，室温下保质期1年。使用说明： 1、 将分离胶I和分离胶II等比例混合（参照附表1），然后将所需的TEMED和10% APS加到混合溶液中（参照附表1），并混合均匀，灌入制胶板内。2、 在制胶板加入适量的醇或水压平分离胶，待分离胶凝固后，倒去上层的水或醇。3、 将浓缩胶I和浓缩胶II等比例混合（参照附表1），然后将所需的TEMED和10% APS加到混合溶液中（参照附表1），并混合均匀。4、 将配置好的浓缩胶预混液加入制作好分离胶的胶板内，缓慢插入干净的梳齿。 5、 待浓缩胶凝固后，拔去梳齿即可用于电泳。备注： 1、 此试剂盒常温存放即可；2、 TEMED和10%APS已包含在试剂盒中，请尽量使用新鲜配制的APS溶液； 3、 在制备分离胶和浓缩胶溶液时，注意避免气泡的产生；4、 制备好的凝胶可以用一张用去离子水浸湿的纸巾盖住，并将其储存在一个密封的塑料袋中，4 ℃可保存四个星期；5、 每个试剂盒可制备的凝胶数量：玻璃板大（9*7cm） 0.75 mm 玻璃板 1.0 mm 玻璃板 1.5 mm 玻璃板 制胶数 100块胶 65块胶 45块胶 关于凝胶速度说明： Ø 实验证明，凝胶速度与温度有显著的正相关性。同等条件下，温度越高，凝胶速度越快。比如25℃时凝胶显著快于15℃。同理，在37℃时，凝胶会进一步加快。如果要加速凝胶，可考虑提高凝胶温度（把胶放进37°培养箱）。Ø APS容易分解失效，APS的效力直接影响凝胶快慢，请尽量使用新配制的APS溶液；另外，如果制作的板子漏胶，也会影响分离胶的凝胶。Ø APS和TEMED在整个凝胶过程起催化加速作用，但添加过多，胶的柔性会降低。“附表1”APS和TEMED的添加量，正常情况会使凝胶在半小时内完成，并确保胶的柔韧性。关于电泳电压的说明： Ø 本试剂盒最适电压为160-200V，可承受的最高分离电压为260V，建议客户根据自己的实验条件和实验目选择电泳电压。附表1：制备胶所需体积列表 0.75mm 玻璃板 (n=制备胶个数) 1.0mm 玻璃板 (n=制备胶个数) 1.5mm 玻璃板 (n=制备胶个数) 浓缩胶 分离胶 浓缩胶 分离胶 浓缩胶 分离胶 分离胶I / 2 ml x n / 3 ml x n / 4 ml x n 分离胶II / 2 ml x n / 3 ml x n / 4 ml x n 浓缩胶I 1 ml x n / 1 ml x n / 1.5 ml x n / 浓缩胶II 1 ml x n / 1 ml x n / 1.5ml x n / TEMED 2 µl x n 2 µl x n 2 µl x n 3 µl x n 3 µl x n 4 µl x n 10% APS 20 µl x n 40 µl x n 20 µl x n 60 µl x n 30 µl x n 80 µl x n

genefist，GF1800-06，6% PAGE预混胶试剂盒

279.00元

产品描述 Ø 此试剂盒用于制备PAGE凝胶，快速、便捷；Ø 所制备的凝胶可用于变性和非变形蛋白质电泳分析；Ø 有6%、8%、10%、12%、15%五个不同浓度可供选择；Ø 预混配方，无需计算所需溶液量，无需稀释，只需两种液体等比例混合，简化传统制胶流程，缩短制胶时间；Ø 可耐受最高260V分离电压，将传统的凝胶电泳实验从1-2小时缩短至30分钟，且条带清晰、锐利；Ø 高效的蛋白转移能力，保证Western blot 数据的高度可靠性；Ø 每盒最多可制胶100块，超高性价比；Ø 不用冷藏或冷冻存贮，室温下保质期1年。使用说明： 1、 将分离胶I和分离胶II等比例混合（参照附表1），然后将所需的TEMED和10% APS加到混合溶液中（参照附表1），并混合均匀，灌入制胶板内。2、 在制胶板加入适量的醇或水压平分离胶，待分离胶凝固后，倒去上层的水或醇。3、 将浓缩胶I和浓缩胶II等比例混合（参照附表1），然后将所需的TEMED和10% APS加到混合溶液中（参照附表1），并混合均匀。4、 将配置好的浓缩胶预混液加入制作好分离胶的胶板内，缓慢插入干净的梳齿。 5、 待浓缩胶凝固后，拔去梳齿即可用于电泳。备注： 1、 此试剂盒常温存放即可；2、 TEMED和10%APS已包含在试剂盒中，请尽量使用新鲜配制的APS溶液； 3、 在制备分离胶和浓缩胶溶液时，注意避免气泡的产生；4、 制备好的凝胶可以用一张用去离子水浸湿的纸巾盖住，并将其储存在一个密封的塑料袋中，4 ℃可保存四个星期；5、 每个试剂盒可制备的凝胶数量：玻璃板大（9*7cm） 0.75 mm 玻璃板 1.0 mm 玻璃板 1.5 mm 玻璃板 制胶数 100块胶 65块胶 45块胶 关于凝胶速度说明： Ø 实验证明，凝胶速度与温度有显著的正相关性。同等条件下，温度越高，凝胶速度越快。比如25℃时凝胶显著快于15℃。同理，在37℃时，凝胶会进一步加快。如果要加速凝胶，可考虑提高凝胶温度（把胶放进37°培养箱）。Ø APS容易分解失效，APS的效力直接影响凝胶快慢，请尽量使用新配制的APS溶液；另外，如果制作的板子漏胶，也会影响分离胶的凝胶。Ø APS和TEMED在整个凝胶过程起催化加速作用，但添加过多，胶的柔性会降低。“附表1”APS和TEMED的添加量，正常情况会使凝胶在半小时内完成，并确保胶的柔韧性。关于电泳电压的说明： Ø 本试剂盒最适电压为160-200V，可承受的最高分离电压为260V，建议客户根据自己的实验条件和实验目选择电泳电压。附表1：制备胶所需体积列表 0.75mm 玻璃板 (n=制备胶个数) 1.0mm 玻璃板 (n=制备胶个数) 1.5mm 玻璃板 (n=制备胶个数) 浓缩胶 分离胶 浓缩胶 分离胶 浓缩胶 分离胶 分离胶I / 2 ml x n / 3 ml x n / 4 ml x n 分离胶II / 2 ml x n / 3 ml x n / 4 ml x n 浓缩胶I 1 ml x n / 1 ml x n / 1.5 ml x n / 浓缩胶II 1 ml x n / 1 ml x n / 1.5ml x n / TEMED 2 µl x n 2 µl x n 2 µl x n 3 µl x n 3 µl x n 4 µl x n 10% APS 20 µl x n 40 µl x n 20 µl x n 60 µl x n 30 µl x n 80 µl x n

ExCell 胎牛血清（标准级）500ml 适用于增殖旺盛的常规细胞系的培养。

3200.00元

本血源来自依科赛公司精心挑选的国内优质血源地，经过与国际知名品牌同样的3级100 nm过滤，符合《中华人民共和国药典》标准，具有极佳的促细胞生长效果，满足部分科研和工业生产的需要。适用于增殖旺盛的常规细胞系的培养 产品特点：精选国内血源国际一流GMP生产线产品符合《中华人民共和国药典》标准内毒素含量≤10EU/ml; 总蛋白含量≤5.0%(w/v)适用于增殖旺盛的常规细胞系的培养

ExCell(SERUM), FSS500, Fetal Bovine Serum ( Standard) 500ml

3000.00元